Childrens Brittle Bone Foundation

Four weeks after labeling myelin lipids with an intraneural injection of 3H-acetate, sciatic nerves were crushed, and the distribution of radiolabeled myelin lipids was followed by autoradiography from 1 d to 10 weeks later. Just prior to crush, silver grains were localized to the myelin sheath. Three days after crush, axons were degenerating and myelin sheaths were breaking down; silver grains appeared over lipid droplets within Schwann cells, fibroblasts, and macrophages. One week after crush the basal-lamina-delimited Schwann-cell tubes (Büngner bands) contained myelin debris, and some tubes already contained regenerating axons. Schwann cells were often displaced to the periphery of the tubes by phagocytes containing heavily labeled myelin debris; extratubal macrophages within the endoneurium contained labeled lipid droplets but no myelin debris. Two weeks after nerve crush silver grains were associated with newly formed myelin around regenerating axons. Many extratubal endoneurial macrophages now contained labeled myelin debris and lipid droplets. By 3 weeks myelination of regenerating axons was advanced, and the myelin sheaths were well labeled. Extratubal macrophages had become the major labeled structure within the nerve because they contained large amounts of labeled myelin debris and lipid droplets. From 4 to 10 weeks after nerve crush the new myelin sheaths continued to thicken and to be well labeled. Debris-laden extratubal macrophages remained the major site of labeled material within the endoneurium. Our results confirm that there is reutilization of myelin cholesterol by Schwann cells to form new myelin, and indicate that some lipid catabolism takes place in Schwann cells and endoneurial fibroblasts prior to infiltration of the nerve by macrophages. However, most of the myelin debris is phagocytized by macrophages within 1-2 weeks following nerve injury. These debris-laden macrophages persist within the nerve for many weeks, indicating that much of the salvaged cholesterol is not reutilized for myelin regeneration.

The idiopathic inflammatory myopathies (IIM) are acquired muscle diseases characterized by muscle weakness and inflammation on muscle biopsy. Clinicoserologic classifications do not take muscle histology into account to distinguish the subsets of IIM. Our objective was to determine the pathologic features of each serologic subset of IIM and to correlate muscle biopsy results with the clinicoserologic classification defined by Troyanov et al, and with the final diagnoses. We retrospectively studied a cohort of 178 patients with clinicopathologic features suggestive of IIM with the exclusion of inclusion body myositis. At the end of follow-up, 156 of 178 cases were still categorized as IIM: pure dermatomyositis, n = 44; pure polymyositis, n = 14; overlap myositis, n = 68; necrotizing autoimmune myopathy, n = 8; cancer-associated myositis, n = 18; and unclassified IIM, n = 4. The diagnosis of IIM was ruled out in the 22 remaining cases. Pathologic dermatomyositis was the most frequent histologic picture in all serologic subsets of IIM, with the exception of patients with anti-Ku or anti-SRP autoantibodies, suggesting that it supports the histologic diagnosis of pure dermatomyositis, but also myositis of connective tissue diseases and cancer-associated myositis. Unspecified myositis was the second most frequent histologic pattern. It frequently correlated with overlap myositis, especially with anti-Ku or anti-PM-Scl autoantibodies. Pathologic polymyositis was rare and more frequently correlated with myositis mimickers than true polymyositis. The current study shows that clinicoserologic and pathologic data are complementary and must be taken into account when classifying patients with IIM patients. We propose guidelines for diagnosis according to both clinicoserologic and pathologic classifications, to be used in clinical practice.

The exotic disease white pine blister rust (caused by Cronartium ribicola) damages and kills whitebark pine (Pinus albicaulis), even in the extreme environments of alpine treeline communities. We surveyed P. albicaulis trees and tree islands for blister rust in 2 distinct alpine treeline communities in Montana, USA, and examined meso- and microtopographic factors potentially related to the climatic requirements for blister rust infection. For each of 60 sampling plots, we created high-resolution digital elevation models, derived microtopography variables, and compared these and distance to water feature variables with blister rust occurrence and intensity (number of cankers per infected tree) for every sampled P. albicaulis tree. Infection rates were 19% (of 328 sampled trees) and 24% (of 585 sampled trees) at the 2 sites. Tree island P. albicaulis had higher infection percentages than solitary trees. Using Bayesian analysis and a zero-inflated Poisson regression model, we determined that solar radiation and moisture-related variables correlated with both presence and number of blister rust cankers on P. albicaulis. Site factors that influence moisture, such as local topography, hydrology, and climate, differed between the 2 treeline study areas, which may account for the model variability.

OBJECTIVE: In patients with chronic radicular pain, we aimed to evaluate subgroup differences in 1-yr response to transforaminal epidural steroid injection. DESIGN: In this longitudinal cohort study of 100 subjects, 170 transforaminal epidural steroid injections were performed for 1 yr. The sample was stratified by type of disc herniation (protrusion n = 57, extrusion n = 27), by location of disc herniation (central/subarticular n = 60, foraminal n = 24), by grade of nerve root compression (low-grade compression n = 61, high-grade subarticular nerve compression n = 14, high-grade foraminal nerve compression n = 25), and by positive Slump test (n = 67). Treatment response was evaluated by visual analogue scale leg pain and self-reported disability (Oswestry Disability Index). Logistic regression was used to analyze the predictive value of baseline characteristics including the stratified subgroups. RESULTS: High-grade subarticular nerve compression predicted the 1-yr improvement in both visual analogue scale leg pain (P = 0.046) and Oswestry Disability Index (P = 0.027). Low age (P < 0.001), short duration of leg pain (P = 0.015), and central/subarticular disc herniation (P = 0.017) predicted improvement in Oswestry Disability Index. CONCLUSIONS: In patients treated with one or several transforaminal epidural steroid injections due to chronic lumbar radicular pain, clinical findings failed to predict the 1-yr treatment response. Low age, short duration of leg pain, central/subarticular disc herniation, and high-grade subarticular nerve compression predicted a favorable 1-yr response to transforaminal epidural steroid injection.

The identity of putative oxytocin receptors visualized in autoradiographic studies of primate brain is unclear because the ligand used is much less selective in primate than rodent brains. This study tests the feasibility of utilizing a new monoclonal antibody (MoAb) developed against human uterine OTRs to visualize OTRs in primate brain. A block containing ventral hypothalamus of cynomolgus macaque brain, paraformaldehyde, glutaraldehyde-fixed and paraffin-embedded, and positive control tissue (human endometrium) were sectioned at 8 microm and studied with immunohistochemistry. OTRs were located in fibers in septal nucleus and in both cell bodies and fibers of preoptic area. These results indicate that OTRs in primate brain may be visualized with this MoAb, and are located in primate brain sites linked to the regulation of social behavior.

Ecological systems are facing major diversity losses in this century owing to Anthropogenic effects. Habitat loss, overexploitation of resources, invasion and pollution are rapidly jeopardizing the survival of whole communities. It has been recently suggested that a potential approach to flatten the curve of species extinction and prevent catastrophic shifts would involve the engineering of one selected species within one of these communities. Such possibility has started to become part of potential intervention scenarios to preserve biodiversity. Despite its potential, very little is known about the actual dynamic responses of complex ecological networks to the introduction of a synthetic strains derived from a resident species. In this paper, we address this problem by modelling the response of a community to the addition of a synthetic strain derived from a member of a stable ecosystem. We show that the community interaction matrix largely limits the spread of the engineered strain, thus suggesting that species diversity acts as an ecological firewall. The implications for future scenarios of ecosystem engineering are outlined. This article is part of the theme issue 'Ecological complexity and the biosphere: the next 30 years'.

PURPOSE: To analyze, histomorphologically, the influence of the geometry of nanostructured hydroxyapatite and alginate (HAn/Alg) composites in the initial phase of the bone repair. METHODS: Fifteen rats were distributed to three groups: MiHA - bone defect filled with HAn/Alg microspheres; GrHA - bone defect filled with HAn/Alg granules; and DV - empty bone defect; evaluated after 15 days postoperatively. The experimental surgical model was the critical bone defect, ≅8.5 mm, in rat calvaria. After euthanasia the specimens were embedded in paraffin and stained with hematoxylin and eosin, picrosirius and Masson-Goldner's trichrome. RESULTS: The histomorphologic analysis showed, in the MiHA, deposition of osteoid matrix within some microspheres and circumjacent to the others, near the bone edges. In GrHA, the deposition of this matrix was scarce inside and adjacent to the granules. In these two groups, chronic granulomatous inflammation was noted, more evident in GrHA. In the DV, it was observed bone neoformation restricted to the bone edges and formation of connective tissue with reduced thickness in relation to the bone edges, throughout the defect. CONCLUSION: The geometry of the biomaterials was determinant in the tissue response, since the microspheres showed more favorable to the bone regeneration in relation to the granules.

The purpose of the present paper consists in proposing and discussing a double probabilistic representation for a porous media equation in the whole space perturbed by a multiplicative colored noise. For almost all random realizations $ω$, one associates a stochastic differential equation in law with random coefficients, driven by an independent Brownian motion. The key ingredient is a uniqueness lemma for a linear SPDE of Fokker-Planck type with measurable bounded (possibly degenerated) random coefficients.

Sullivan, Daniel M.Div.; Langmoen, Iver M.D.; Adams, Christopher B. T. M.D.; Sainte-Rose, Christian M.D.; Apuzzo, Michael L. J. M.D. Author Information

Some time lag, however small between time of death or the discovery of the body (and pending medicolegal autopsy), would appear to be indispensible and justified in each case for reasons of investigation. This time interval is reduced to a required minimum, depending upon the competency of crime investigative machinery and the concern of well-intentioned individuals. In a developing country like India, this can cause serious problems. The time lag interval between the discovery of the body and the autopsy is often very long in India, and may be attributed to reasons which are outside the legitimate needs of investigation, ranging from outright incompetence to cool callousness, willful omissions, or machinations based on unfair motivations and political compulsion. Long distances, proper communication, and availability of transportation all play their parts in the need for proper indentification and autopsy examination. In addition to delays in performing the autopsies, there are numerous instances of unnatural, sudden, and suspicious deaths, where postmortem examination is never done. These reasons range from those that are seemingly innocent to those which are grossly unfair or dishonest. The medicolegal postmortem index can vary between 20 and 80% in different areas of India.

My book Cosmopolitan Islanders derives from the Inaugural Lecture I delivered in 2008 as Regius Professor of Modern History in Cambridge. The brief of such a lecture is a tricky one – you have to say something about yourself, something about your field, and something about the discipline of History, and you have to appeal both to colleagues and to the wider world. In addition, in both Oxford and Cambridge, the only two universities with Regius Chairs of History, there have been many famous Inaugurals, by historians as varied as J. B. Bury, Hugh Trevor-Roper and, most famous of all, Lord Acton. In some universities, where professors are all-powerful, the Inaugural has served as a means of laying down the law about how the subject should be taught and researched. But in recent times this has been rare, and it has never really been true in Oxford or Cambridge; the one time that an Oxford Regius, the seventeenth-century specialist Sir Charles Firth, tried this, he ran into a huge amount of trouble and was more or less ostracised in the Faculty for the rest of his career.

In normal stomach, MUC5AC and MUC6 apomucins are associated with Lewis types 1 and 2, respectively, and this association is lost during gastric carcinogenesis. The expression of gastric (MUC5AC, MUC6) and intestinal (MUC2, MUC4) apomucins and Lewis antigens during gastric development, using single and double labeling immunohistochemistry on fetal tissues (15–41 weeks), was analyzed and related to the tumor expression patterns. Apomucin expression in other fetal tissues was also analyzed. In gastric samples, MUC2 is detected in 14 of 19 showing no correlation with fetal age, and MUC4 is not detected. MUC5AC and MUC6 are always highly detected and are coexpressed and associated with both types of Lewis antigens. These patterns change progressively with the development of the adult gastric morphology. MUC2 is detected in the small intestine, colon, and pancreas; MUC4 is expressed in the colon; MUC5AC is detected in the small intestine; and MUC6 is found in the duodenum and pancreas. The patterns of apomucin expression and association with Lewis antigens during development are complex, but there is a trend toward the establishment of the adult pattern, with the exception of MUC4, which is not detected. These patterns found in fetal stomach indicate that alterations reported in gastric tumors do not fully recapitulate a developmental phenotype.

Sinclair Lewis's critical reputation could not easily be lower than it is at present. In the discussion that follows I want to suggest that a radical re-evaluation of this reputation, and of Lewis's achievement as a novelist, is necessary; not just because he ought to be read and studied seriously in his own right, but also because in his best fiction he addresses very important issues of narrative technique and of historical and structural analysis. And I believe that without a proper understanding of Lewis's engagement with his material in these areas, most notably in Main Street (1920) and Babbitt (1922), the two texts I want to concentrate on here, any account of the re-shaping of American fiction in the years after the First World War must be seriously incomplete. Lewis has proved to be an extremely easy writer to dismiss from any literary or intellectual canon, and not without good reason. His fictions are direct, accessible, and for the most part actively simple. Like Wells and Bennett in England he is a provincial writer of materialist romances, apparently left behind by Modernism; like Upton Sinclair he is a clumsy and over-productive fictionaliser of obvious social problems; in the 1920s he is a man of middle age writing stories about middle-aged characters for a middle-aged readership in a literary and intellectual climate obsessed and characterized by youth; he is a “wildly inconsistent writer, who in his weakest work unquestionably justifies Schorer's description of him as “one of the worst writers in modern American Literature.” And probably more than any other twentieth-century writer of comparable stature, Lewis has been a victim of literary history-writing. With only the very weak platform of spectacular popular success to support him, he stands as the clearest (and unfortunately also the most vociferous) representative of the American fiction that Hemingway and Fitzgerald and their apologists were careful to be seen to be superseding in the 1920s and 1930s.

From the Department of Physical Medicine and Rehabilitation, Stanford University, Redwood City, California (KFB); and Department of Neurology, Boston University School of Medicine, Boston, Massachusetts (MDP). All correspondence and requests for reprints should be addressed to: Kevin F. Barrette, MD, Department of Physical Medicine and Rehabilitation, Stanford University, 450 Broadway St, M/C 6342, Redwood City, CA 94063. Financial disclosure statements have been obtained, and no conflicts of interest have been reported by the authors or by any individuals in control of the content of this article.

ABSTRACT During January 1911 two separate expeditions came ashore within ten days of each other in Antarctica. Both were hoping to achieve the South Pole. Robert Falcon Scott's decision to establish his British Antarctic Expedition's winter quarters on Ross Island close to routes explored on his first Antarctic expedition was signalled in advance. Scott had received notification of the Norwegian Roald Amundsen's intention to head for Antarctica but did not know where he planned to land. At 00.05 am on 4 February 1911 the British expedition's vessel Terra Nova unexpectedly came across Amundsen's Fram moored to the ice edge in the Bay of Whales. 14 hours later Terra Nova departed, taking news of Amundsen's location and plans, to deliver to Scott. For those on Terra Nova who kept diaries, the event filled days surrounding the encounter spurred the recording of thoughts, emotions and conversations as well as descriptions: eye witness accounts, allowing us to gain an appreciation of the situation as it was. This material from the British side, together with letters, and diary entries from earlier in the voyage, reveals a more complex account than do expedition narratives published subsequently by several of the participants. Terra Nova 's passage along the edge of the Ross Ice Shelf in February 1911 had, in fact, come close to being cancelled. Terra Nova 's commander as instructed by Scott was heading for Balloon Bight on 3 February, not the Bay of Whales. Even the ship's departure after a short stay, with everyone still on board, became a matter for intense debate. In addition, the generally very positive attitude of the British towards the Norwegians as expressed in diaries hardened subsequently, at least for some. This paper's focus is on the responses of those on Terra Nova to the encounter. Those of the Norwegians are being explored by other researchers.

This chapter studies the installation of church organs in Italian Observant churches, and investigates how this to some extent was legitimized or not within Observant discourses of religious discipline and poverty. All this also took place in light of the realization that the organ was able to contribute to the outward reputation of the communities in question, and could stir up devout emotions and facilitate religious conversion.

Existence and uniqueness of solutions to the stochastic porous media equation $dX-\Dψ(X) dt=XdW$ in $\rr^d$ are studied. Here, $W$ is a Wiener process, $ψ$ is a maximal monotone graph in $\rr\times\rr$ such that $ψ(r)\le C|r|^m$, $\ff r\in\rr$, $W$ is a coloured Wiener process. In this general case the dimension is restricted to $d\ge 3$, the main reason being the absence of a convenient multiplier result in the space $\calh=\{φ\in\mathcal{S}'(\rr^d);\ |ξ|(\calfφ)(ξ)\in L^2(\rr^d)\}$, for $d\le2$. When $ψ$ is Lipschitz, the well-posedness, however, holds for all dimensions on the classical Sobolev space $H^{-1}(\rr^d)$. If $ψ(r)r\geρ|r|^{m+1}$ and $m=\frac{d-2}{d+2}$, we prove the finite time extinction with strictly positive probability.

Vegetative desiccation tolerance (VDT) has evolved independently across vascular plants, but its genetic basis remains poorly understood. Although VDT is associated with expansion of the ELIP gene family, the contribution of other lineage-specific expansions is unclear. We assembled genomes for Xerophyta elegans and Xerophyta humilis, identifying expanded gene families largely involved in chlorophyll metabolism and abscisic acid mediated stress responses. Using a dense dehydration rehydration transcriptome series in X. elegans seedlings, we reconstructed the regulatory network underlying VDT. Transcription factors from the ABF, ZAT and HSFC families were associated with early responses to desiccation. Key regulators of the seed maturation programme, including NAC transcription factors (ATAF1 and ANAC032), DOG genes and the trihelix factor ASIL1, were also implicated. These findings indicate that VDT arises through integration of abiotic stress signalling with rewiring of the seed maturation network, enabling desiccation tolerance in vegetative tissues.

SNDROME DE BURNOUT NA ENFERMAGEM changes due to stress levels due to work overload, night shifts, low wages, poor working conditions, difficulties in interpersonal relationships in the work environment, physical and emotional exhaustion , which ends up leading the professional to a loss of motivation and impairing the performance of tasks, and not treated in a timely manner, has high levels of suicidal behavior.Through a bibliographical and systematic review of the literature, we observed that its etiology was clarified and that the treatment should be carried out in a multidisciplinary way.This is a literature review study with a narrative approach.

Essa pesquisa é fruto do trabalho de conclusão de curso e tem como objetivo analisar a presença das mulheres em uma coleção de livros didáticos de Matemática, visto que, no cenário escolar e particularmente nas salas de aula, essas mulheres não têm visibilidade. Nesse contexto, pretende-se contribuir com a prática do educador matemático ao propor-lhes refletir sobre a importância de abordar as participações de mulheres na Matemática na sala de aula, bem como para o meio científico uma vez que existem ainda lacunas presentes no tratamento dessa temática. Esta pesquisa segue uma abordagem qualitativa e terá como técnica de coleta de dados a análise documental. A revisão de literatura busca discutir a importância da história das mulheres na Matemática se fazer presente nos livros didáticos. Além disso, apresenta como a história dessas mulheres pode contribuir no processo de ensino-aprendizagem. Os resultados obtidos através da análise de dados mostram como os livros didáticos de Matemática ignoram as contribuições de mulheres no desenvolvimento da Matemática. Palavras-chave: História da Matemática. Contribuições femininas. Livro didático.