Far Eastern Federal University

autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.

Abstract. The global methane (CH4) budget is becoming an increasingly important component for managing realistic pathways to mitigate climate change. This relevance, due to a shorter atmospheric lifetime and a stronger warming potential than carbon dioxide, is challenged by the still unexplained changes of atmospheric CH4 over the past decade. Emissions and concentrations of CH4 are continuing to increase, making CH4 the second most important human-induced greenhouse gas after carbon dioxide. Two major difficulties in reducing uncertainties come from the large variety of diffusive CH4 sources that overlap geographically, and from the destruction of CH4 by the very short-lived hydroxyl radical (OH). To address these difficulties, we have established a consortium of multi-disciplinary scientists under the umbrella of the Global Carbon Project to synthesize and stimulate research on the methane cycle, and producing regular (∼ biennial) updates of the global methane budget. This consortium includes atmospheric physicists and chemists, biogeochemists of surface and marine emissions, and socio-economists who study anthropogenic emissions. Following Kirschke et al. (2013), we propose here the first version of a living review paper that integrates results of top-down studies (exploiting atmospheric observations within an atmospheric inverse-modelling framework) and bottom-up models, inventories and data-driven approaches (including process-based models for estimating land surface emissions and atmospheric chemistry, and inventories for anthropogenic emissions, data-driven extrapolations). For the 2003–2012 decade, global methane emissions are estimated by top-down inversions at 558 Tg CH4 yr−1, range 540–568. About 60 % of global emissions are anthropogenic (range 50–65 %). Since 2010, the bottom-up global emission inventories have been closer to methane emissions in the most carbon-intensive Representative Concentrations Pathway (RCP8.5) and higher than all other RCP scenarios. Bottom-up approaches suggest larger global emissions (736 Tg CH4 yr−1, range 596–884) mostly because of larger natural emissions from individual sources such as inland waters, natural wetlands and geological sources. Considering the atmospheric constraints on the top-down budget, it is likely that some of the individual emissions reported by the bottom-up approaches are overestimated, leading to too large global emissions. Latitudinal data from top-down emissions indicate a predominance of tropical emissions (∼ 64 % of the global budget, < 30° N) as compared to mid (∼ 32 %, 30–60° N) and high northern latitudes (∼ 4 %, 60–90° N). Top-down inversions consistently infer lower emissions in China (∼ 58 Tg CH4 yr−1, range 51–72, −14 %) and higher emissions in Africa (86 Tg CH4 yr−1, range 73–108, +19 %) than bottom-up values used as prior estimates. Overall, uncertainties for anthropogenic emissions appear smaller than those from natural sources, and the uncertainties on source categories appear larger for top-down inversions than for bottom-up inventories and models. The most important source of uncertainty on the methane budget is attributable to emissions from wetland and other inland waters. We show that the wetland extent could contribute 30–40 % on the estimated range for wetland emissions. Other priorities for improving the methane budget include the following: (i) the development of process-based models for inland-water emissions, (ii) the intensification of methane observations at local scale (flux measurements) to constrain bottom-up land surface models, and at regional scale (surface networks and satellites) to constrain top-down inversions, (iii) improvements in the estimation of atmospheric loss by OH, and (iv) improvements of the transport models integrated in top-down inversions. The data presented here can be downloaded from the Carbon Dioxide Information Analysis Center (http://doi.org/10.3334/CDIAC/GLOBAL_METHANE_BUDGET_2016_V1.1) and the Global Carbon Project.

Skyrmions are topologically protected entities in magnetic materials which have the potential to be used in spintronics for information storage and processing. However, Skyrmions in ferromagnets have some intrinsic difficulties which must be overcome to use them for spintronic applications, such as the inability to move straight along current. We show that Skyrmions can also be stabilized and manipulated in antiferromagnetic materials. An antiferromagnetic Skyrmion is a compound topological object with a similar but of opposite sign spin texture on each sublattice, which, e.g., results in a complete cancellation of the Magnus force. We find that the composite nature of antiferromagnetic Skyrmions gives rise to different dynamical behavior due to both an applied current and temperature effects.

We highlight the progress, current status, and open challenges of QCD-driven physics, in theory and in experiment. We discuss how the strong interaction is intimately connected to a broad sweep of physical problems, in settings ranging from astrophysics and cosmology to strongly coupled, complex systems in particle and condensed-matter physics, as well as to searches for physics beyond the Standard Model. We also discuss how success in describing the strong interaction impacts other fields, and, in turn, how such subjects can impact studies of the strong interaction. In the course of the work we offer a perspective on the many research streams which flow into and out of QCD, as well as a vision for future developments.

The present study focused on the elucidation of the putative anticancer potential of quercetin. The anticancer activity of quercetin at 10, 20, 40, 80 and 120 µM was assessed in vitro by MMT assay in 9 tumor cell lines (colon carcinoma CT‑26 cells, prostate adenocarcinoma LNCaP cells, human prostate PC3 cells, pheocromocytoma PC12 cells, estrogen receptor‑positive breast cancer MCF‑7 cells, acute lymphoblastic leukemia MOLT‑4 T‑cells, human myeloma U266B1 cells, human lymphoid Raji cells and ovarian cancer CHO cells). Quercetin was found to induce the apoptosis of all the tested cancer cell lines at the utilized concentrations. Moreover, quercetin significantly induced the apoptosis of the CT‑26, LNCaP, MOLT‑4 and Raji cell lines, as compared to control group (P<0.001), as demonstrated by Annexin V/PI staining. In in vivo experiments, mice bearing MCF‑7 and CT‑26 tumors exhibited a significant reduction in tumor volume in the quercetin‑treated group as compared to the control group (P<0.001). Taken together, quercetin, a naturally occurring compound, exhibits anticancer properties both in vivo and in vitro.

The presented review summarizes nearly two decades of studies on femtosecond laser-induced breakdown spectrometry (fs-LIBS).

This article represents an overview on microbial production of vitamin B2 (riboflavin) with detailed characterization of the riboflavin-producing microorganisms and its biosynthesis pathways known up to 2020. Riboflavin is a crucial micronutrient that is a precursor to coenzymes flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) required for biochemical reactions in all living cells. Among for decades, one of the most applications of riboflavin that it has been used worldwide as animal and human nutritional supplement. The latest researches on riboflavin production via fermentation process is required for the development of new and improved microbial strains using biotechnology and metabolic engineering techniques to increase the yield of vitamin B2. In this paper, we describe well-known industrial microbial producers Ashbya gossypii, Bacillus subtilis, and Candida spp., and summary of its biosynthetic pathway optimization through genetic and metabolic engineering, combined with random chemical mutagenesis and rational medium components to increase riboflavin production.

Geopolymer concretes (geocretes) are considered as eco-friendly materials for various building applications. Geocrete has high early strength, less consumption of natural resources, cost-effectiveness, capacity to form different structural configurations and to remain intact for extended periods without repair works. Meanwhile, geocretes have still exhibited an unstable behavior over time compared to traditional cementitious composites. To overcome this disadvantage, hundreds of studies have focused on the improvement of the microstructure of geocretes with a wide range of improved durability characteristics. Therefore, the review paper has an objective to make available an inclusive review on the production of supplemental-cementing-materials (SCMs), their economic returns, environmental and durability impacts, the conceptual model for geopolymerization, durability affecting factors, and function and long-term durability properties of geocrete. It is concluded that geocrete demonstrated a better resistance against aggressive environment compared to normal concrete, due to its less porous structures. Moreover, it is found that the strength of alkali-activated concrete was found to improve in chloride environment, unlike OPC based concrete. It is also concluded that, the presence of GGBS and MK in alkali-activated materials reduces its alkali-silica reactivity while it is recommended to use high calcium FA in geocrete production for durable concrete and geopolymerization. The higher the molarity of NaOH solution for a given quantity of Na2SiO3 the faster the geopolymerization process and the higher the compressive strength of concrete corrosion current than the OPC concrete. Further long-term durability studies are required to provide test methods and validation techniques since most studies focus on the 28-day curing regime.

Industrial application of metallic materials is hindered by several shortcomings, such as proneness to corrosion, erosion under abrasive loads, damage due to poor cold resistance, or weak resistance to thermal shock stresses, etc. In this study, using the aluminum-magnesium alloy as an example of widely spread metallic materials, we show that a combination of functional nanoengineering and nanosecond laser texturing with the appropriate treatment regimes can be successfully used to transform a metal into a superhydrophobic material with exceptional mechanical and chemical properties. It is demonstrated that laser chemical processing of the surface may be simultaneously used to impart multimodal roughness and to modify the composition and physicochemical properties of a thick surface layer of the substrate itself. Such integration of topographical and physicochemical modification leads to specific surface nanostructures such as nanocavities filled with hydrophobic agent and hard oxynitride nanoinclusions. The combination of superhydrophobic state, nano- and micro features of the hierarchical surface, and the appropriate composition of the surface textured layer allowed us to provide the surface with the outstanding level of resistance of superhydrophobic coatings to external chemical and mechanical impacts. In particular, experimental data presented in this study indicate high resistance of the fabricated coatings to pitting corrosion, superheated water vapor, sand abrasive wear, and rapid temperature cycling from liquid nitrogen to room temperatures, without notable degradation of superhydrophobic performance.

Extracellular matrix (ECM) is an extraordinarily complex and unique meshwork composed of structural proteins and glycosaminoglycans. The ECM provides essential physical scaffolding for the cellular constituents, as well as contributes to crucial biochemical signaling. Importantly, ECM is an indispensable part of all biological barriers and substantially modulates the interchange of the nanotechnology products through these barriers. The interactions of the ECM with nanoparticles (NPs) depend on the morphological characteristics of intercellular matrix and on the physical characteristics of the NPs and may be either deleterious or beneficial. Importantly, an altered expression of ECM molecules ultimately affects all biological processes including inflammation. This review critically discusses the specific behavior of NPs that are within the ECM domain, and passing through the biological barriers. Furthermore, regenerative and toxicological aspects of nanomaterials are debated in terms of the immune cells-NPs interactions.

Magnetoresistive (MR) sensors have been identified as promising candidates for the development of high-performance magnetometers due to their high sensitivity, low cost, low power consumption, and small size. The rapid advance of MR sensor technology has opened up a variety of MR sensor applications. These applications are in different areas that require MR sensors with different properties. Future MR sensor development in each of these areas requires an overview and a strategic guide. An MR sensor roadmap (non-recording applications) was therefore developed and made public by the Technical Committee of the IEEE Magnetics Society with the aim to provide an research and development (R&D) guide for MR sensors intended to be used by industry, government, and academia. The roadmap was developed over a three-year period and coordinated by an international effort of 22 taskforce members from ten countries and 17 organizations, including universities, research institutes, and sensor companies. In this paper, the current status of MR sensors for non-recording applications was identified by analyzing the patent and publication statistics. As a result, timescales for MR sensor development were established and critical milestones for sensor parameters were extracted in order to gain insight into potential MR sensor applications (non-recording). Five application areas were identified, and five MR sensor roadmaps were established. These include biomedical applications, flexible electronics, position sensing and human–computer interactions, non-destructive evaluation and monitoring, and navigation and transportation. Each roadmap was analyzed using a logistic growth model, and new opportunities were predicted based on the extrapolated curve, forecast milestones, and professional judgment of the taskforce members. This paper provides a framework for MR sensor technology (non-recording applications) to be used for public and private R&D planning, in order to provide guidance into likely MR sensor applications, products, and services expected in the next 15 years and beyond.

Photoplethysmography (PPG) is a noninvasive optical method accepted in the clinical use for measurements of arterial oxygen saturation. It is widely believed that the light intensity after interaction with the biological tissue in vivo is modulated at the heartbeat frequency mainly due to pulsatile variations of the light absorption caused by arterial blood-volume pulsations. Here we report experimental observations, which are not consistent with this model and demonstrate the importance of elastic deformations of the capillary bed in the formation of the PPG waveform. These results provide new insight on light interaction with live tissue. To explain the observations we propose a new model of PPG in which pulse oscillations of the arterial transmural pressure deform the connective-tissue components of the dermis resulting in periodical changes of both the light scattering and absorption. These local changes of the light-interaction parameters are detected as variations of the light intensity returned to a photosensitive camera. Therefore, arterial pulsations can be indirectly monitored even by using the light, which slightly penetrates into the biological tissue.

Disasters are crisis circumstances that put human life in jeopardy. During disasters, public communication infrastructure is particularly damaged, obstructing Search And Rescue (SAR) efforts, and it takes significant time and effort to re-establish functioning communication infrastructure. SAR is a critical component of mitigating human and environmental risks in disasters and harsh environments. As a result, there is an urgent need to construct communication networks swiftly to help SAR efforts exchange emergency data. UAV technology has the potential to provide key solutions to mitigate such disaster situations. UAVs can be used to provide an adaptable and reliable emergency communication backbone and to resolve major issues in disasters for SAR operations. In this paper, we evaluate the network performance of UAV-assisted intelligent edge computing to expedite SAR missions and functionality, as this technology can be deployed within a short time and can help to rescue most people during a disaster. We have considered network parameters such as delay, throughput, and traffic sent and received, as well as path loss for the proposed network. It is also demonstrated that with the proposed parameter optimization, network performance improves significantly, eventually leading to far more efficient SAR missions in disasters and harsh environments.

ABSTRACT During the last decades, the effects of global warming have become apparent also in Europe, causing relevant impacts in many sectors. Under projected future global warming, such a tendency can be expected to persist until the end of this century and beyond. Identifying which climate‐related impacts are likely to increase, and by how much, is an important element of any effective strategy for managing future climate risks. This study investigates whether energy demand for cooling and heating buildings can be expected to increase or decrease under climate change. Two indicators of weather‐related energy consumption for heating and cooling buildings are considered: heating degree‐days (HDD) and cooling degree‐days (CDD). The evolution of these indicators has been analysed based on 11 high‐resolution bias‐adjusted EURO‐CORDEX simulations for two emission representative concentration pathways (RCP4.5 and RCP8.5). Both indicators have been validated over the period 1981–2010 using an independent data set that contains more than 4000 station data, showing very high correlation over most of Europe. Trends of HDD and CDD from 1981 to 2100, together with their uncertainties, are analysed. For both RCPs, all simulations project a significant decrease for HDD, especially over Scandinavia and European Russia, and an increase of CDD which peaks over the Mediterranean region and the Balkans. Overall, degree‐day trends do not show remarkable differences if population weighting is applied. If a constant population scenario is considered, the decrease in HDD will outbalance the increase in CDD in the 21st century over most of Europe. Thus the related energy demand (expressed as Energy Degree‐days, EDD) is expected to decrease. If, however, population projections over the 21st century are included in the calculations, it is shown that despite the persisting warming, EDD will increase over northern Europe, the Baltic countries, Great Britain, Ireland, Benelux, the Alps, Spain, and Cyprus, resulting in an overall increase in EDD over Europe.

Abstract Interaction of light pulses of various durations and intensities with nanoscale photonic structures plays an important role in many applications of nanophotonics for high‐density data storage, ultra‐fast data processing, surface coloring and sensing. A design of optically tunable and reconfigurable structures made from different materials is based on many important physical effects and advances in material science, and it employs the resonant character of light interaction with nanostructures and strong field confinement at the nanoscale. Here we review the recent progress in physics of tunable and reconfigurable nanophotonic structures of different types. We start from low laser intensities that produce weak reversible changes in nanostructures, and then move to the discussion of non‐reversible changes in photonic structures. We focus on three platforms based on metallic, dielectric and hybrid resonant photonic structures such as nanoantennas, nanoparticle oligomers and nanostructured metasurfaces. Main challenges and key advantages of each of the approaches focusing on applications in advanced photonic technologies are also discussed.

This paper discusses the modes of occurrence of critical elements and their origins in the studied coal and associated rock samples (partings and roof strata) from the Datanhao coal mine, Daqingshan Coalfield, Inner Mongolia, northern China. The studied coals are enriched in trace elements including Zr, Hf, Th, Be, F, Zn, Ga, Nb, Mo, Cd, In, Sn, Ta, Hg, W, Pb, and rare earth elements and Y (REY). Zirconium, Nb, Ta, and Hf are largely associated with anatase in the studied coal and non-coal samples. The main carriers of REY, Th, and U in both coal and non-coal samples are Ca-bearing REE-phosphate minerals, most likely monazite, although U is partly associated with organic matter. Fluorine in the Datanhao coals is mainly associated with kaolinite. Most partings identified as volcanic ash-derived tonsteins from the Datanhao Mine exhibit negative Eu anomalies that, along with the common presence of equant-shaped euhedral volcanic quartz (beta-quartz), indicate a felsic volcanic source located in the Xing'an-Mongolia Orogenic Belt. Other probable sources of clastic material into the basin include the pre-Cambrian granites from the adjacent uplands, which may be responsible for the light rare earth element enrichment of the studied deposits, and associated metamorphic rocks from the adjacent uplands. Owing to acidic hydrothermal fluids, which are most likely related to the Yanshanian (Jurassic to Early Cretaceous) intrusive activity and/or low-temperature basinal waters circulating within the coal-bearing unit during the epigenetic stage, most of the coal benches from the Datanhao mine are also relatively enriched in middle rare earth elements. On the other hand, some coal and non-coal bench samples from the middle Datanhao section are enriched in heavy REE. This, along with the presence of abundant syngenetic or early diagenetic carbonate minerals, indicates deposition in an episodic alkaline environment, the origin of which is mostly probably attributed to evaporation and/or saline water inputs resembling a playa lake environment. As the concentrations of Zr, Hf, Nb, and Ta are all close to or higher than the respective industry cut-off grades or standards for these critical elements, the Datanhao coal seam has potential economic significance for these as an important by-product of the coal mining.

Halide-perovskite microlasers have demonstrated fascinating performance owing to their low-threshold lasing at room temperature and low-cost fabrication. However, being synthesized chemically, controllable fabrication of such microlasers remains challenging, and it requires template-assisted growth or complicated nanolithography. Here, we suggest and implement an approach for the fabrication of microlasers by direct laser ablation of a thin film on glass with donut-shaped femtosecond laser beams. The fabricated microlasers represent MAPbBrxIy microdisks with 760 nm thickness and diameters ranging from 2 to 9 μm that are controlled by a topological charge of the vortex beam. As a result, this method allows one to fabricate single-mode perovskite microlasers operating at room temperature in a broad spectral range (550–800 nm) with Q-factors up to 5500. High-speed fabrication and reproducibility of microdisk parameters, as well as a precise control of their location on a surface, make it possible to fabricate centimeter-sized arrays of such microlasers. Our finding is important for direct writing of fully integrated coherent light sources for advanced photonic and optoelectronic circuitry.

In the last few decades, the demand for cement production increased and caused a massive ecological issue by emitting 8% of the global CO2, as the making of 1 ton of ordinary Portland cement (OPC) emits almost a single ton of CO2. Significant air pollution and damage to human health are associated with the construction and cement industries. Consequently, environmentalists and governments have ordered to strongly control emission rates by using other ecofriendly supplemental cementing materials. Rice husk is a cultivated by-product material, obtained from the rice plant in enormous quantities. With no beneficial use, it is an organic waste material that causes dumping issues. Rice husk has a high silica content that makes it appropriate for use in OPC; burning it generates a high pozzolanic reactive rice husk ash (RHA) for renewable cement-based recyclable material. Using cost-effective and commonly obtainable RHA as mineral fillers in concrete brings plentiful advantages to the technical characteristics of concrete and to ensure a clean environment. With RHA, concrete composites that are robust, highly resistant to aggressive environments, sustainable and economically feasible can be produced. However, the production of sustainable and greener concrete composites also has become a key concern in the construction industries internationally. This article reviews the source, clean production, pozzolanic activity and chemical composition of RHA. This literature review also provides critical reviews on the properties, hardening conditions and behaviors of RHA-based concrete composites, in addition to summarizing the research recent findings, to ultimately produce complete insights into the possible applications of RHA as raw building materials for producing greener concrete composites—all towards industrializing ecofriendly buildings.

This review presents a detailed analysis of published research data focused on the pharmacological activity exerted by biologically active compounds isolated from sea cucumbers belonging to the class of Holothuroidea, phylum Echinodermata. The review contains descriptions of the structure, physico-chemical properties and pharmacological effects of these active substances. Particular attention is given to compounds with anticoagulant, antithrombotic, antioxidant, anticancer, anti-infectious, immune-stimulating and anti-ACE (angiotensin converting enzyme) activities as well as to the substances exerting a regulating influence on lipid and carbohydrate metabolism. All these compounds may be considered as prototypes for development of new pharmaceutical substances and medicines.

Recent investigations documented that plants can uptake and process externally applied double-stranded RNAs (dsRNAs), hairpin RNAs (hpRNAs), and small interfering RNAs (siRNAs) designed to silence important genes of plant pathogenic viruses, fungi, or insects. The exogenously applied RNAs spread locally and systemically, move into the pathogens, and induce RNA interference-mediated plant pathogen resistance. Recent findings also provided examples of plant transgene and endogene post-transcriptional down-regulation by complementary dsRNAs or siRNAs applied onto the plant surfaces. Understanding the plant perception and processing of exogenous RNAs could result in the development of novel biotechnological approaches for crop protection. This review summarizes and discusses the emerging studies reporting on exogenous RNA applications for down-regulation of essential fungal and insect genes, targeting of plant viruses, or suppression of plant transgenes and endogenes for increased resistance and changed phenotypes. We also analyze the current understanding of dsRNA uptake mechanisms and dsRNA stability in plant environments.