Kamphaeng Phet Rajabhat University

Viremia titers in serial plasma samples from 168 children with acute dengue virus infection who were enrolled in a prospective study at 2 hospitals in Thailand were examined to determine the role of virus load in the pathogenesis of dengue hemorrhagic fever (DHF). The infecting virus serotype was identified for 165 patients (DEN-1, 46 patients; DEN-2, 47 patients; DEN-3, 47 patients, DEN-4, 25 patients). Patients with DEN-2 infections experienced more severe disease than those infected with other serotypes. Eighty-one percent of patients experienced a secondary dengue virus infection that was associated with more severe disease. Viremia titers were determined for 41 DEN-1 and 46 DEN-2 patients. Higher peak titers were associated with increased disease severity for the 31 patients with a peak titer identified (mean titer of 107.6 for those with dengue fever vs. 108.5 for patients with DHF, P=.01). Increased dengue disease severity correlated with high viremia titer, secondary dengue virus infection, and DEN-2 virus type.

A prospective observational study was conducted to identify early indicators of acute dengue virus infection. Children with fever for <72 h without obvious cause were studied at hospitals in Bangkok and Kamphaeng Phet, Thailand, until resolution of fever. Of 172 evaluable subjects (91% of enrollees), 60 (35%) had dengue, including 32 with dengue fever (DF) and 28 with dengue hemorrhagic fever (DHF). At enrollment, children with dengue were more likely than children with other febrile illnesses (OFI) to report anorexia, nausea, and vomiting and to have a positive tourniquet test, and they had lower total white blood cell counts, absolute neutrophil and absolute monocyte counts, and higher plasma alanine and aspartate (AST) aminotransferase levels than children with OFI. Plasma AST levels were higher in children who developed DHF than in those with DF. These data identify simple clinical and laboratory parameters that help to identify children with DF or DHF.

Dengue hemorrhagic fever (DHF), the most severe form of illness following infection with a dengue virus, is characterized by plasma leakage, thrombocytopenia, and hepatic inflammation. The interrelationships among virus burden, immune activation, and development of DHF were examined in 54 children with secondary dengue-3 virus infections participating in a prospective, hospital-based study. DHF was associated with higher mean plasma viremia early in illness and earlier peak plasma interferon-gamma levels. Maximum plasma viremia levels correlated with the degree of plasma leakage and thrombocytopenia. Maximum plasma levels of interleukin (IL)-10 and soluble tumor necrosis factor receptor-II correlated with the degree of thrombocytopenia, independently of viremia levels. Hepatic transaminase elevation correlated with plasma soluble IL-2 receptor levels and not with viremia levels. Quantitative differences in virus burden and host immune responses, and the timing of type 1 cytokine responses, have differing influences on the severity of disease manifestations during secondary dengue-3 virus infections.

This work was performed at the Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.

This comprehensive review provides an in-depth overview of the green (biological) synthesis, characterization, and biosensor applications of metal nanoparticles (NPs). Because of their unique physical and chemical properties, high surface area, and nanoscale size, NPs have become crucial in various fields. The review emphasizes the synthesis, properties, and applications of several metal NPs, particularly silver (AgNPs), gold (AuNPs), platinum (PtNPs), copper (CuNPs), zinc oxide (ZnONPs), iron oxide (FeONPs), and palladium (PdNPs). Green synthesis methods, a truly innovative approach, utilize biological substances such as plant extracts, bacteria, fungi, and yeast. These methods offer environmentally friendly and biologically compatible alternatives to conventional chemical synthesis techniques. This review details these sustainable approaches and their advantages over traditional methods. These natural sources provide an abundant, cost-effective, and environmentally sustainable alternative for NP production. The importance of thorough characterization of nanoparticles is also discussed, highlighting techniques such as transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and UV–Vis spectroscopy to analyze the size, shape, surface properties, structural integrity, and optical behavior of the NPs. The review highlights the vast potential of metal NPs in biosensors, which play a critical role in medical diagnostics, environmental monitoring, and food safety. Incorporating metal NPs in electrochemical, optical, thermometric, and piezoelectric biosensors significantly enhances sensitivity and specificity, enabling rapid and real-time detection of various analytes.

We examined the memory cytotoxic T-lymphocytic (CTL) responses of peripheral blood mononuclear cells (PBMC) obtained from patients in Thailand 12 months after natural symptomatic secondary dengue virus infection. In all four patients analyzed, CTLs were detected in bulk culture PBMC against nonstructural dengue virus proteins. Numerous CD4+ and CD8+ CTL lines were generated from the bulk cultures of two patients, KPP94-037 and KPP94-024, which were specific for NS1.2a (NS1 and NS2a collectively) and NS3 proteins, respectively. All CTL lines derived from both patients were cross-reactive with other serotypes of dengue virus. The CD8+ NS1.2a-specific lines from patient KPP94-037 were HLA B57 restricted, and the CD8+ NS3-specific lines from patient KPP94-024 were HLA B7 restricted. The CD4+ CTL lines from patient KPP94-037 were HLA DR7 restricted. A majority of the CD8+ CTLs isolated from patient KPP94-024 were found to recognize amino acids 221 to 232 on NS3. These results demonstrate that in Thai patients after symptomatic secondary natural dengue infections, CTLs are mainly directed against nonstructural proteins and are broadly cross-reactive.

Plasma leakage in dengue hemorrhagic fever (DHF) is associated with elevated plasma levels of cytokines. To define further the contribution of immune activation to DHF and the source of cytokines, we analyzed the production of cytokines in peripheral blood mononuclear cells (PBMC) obtained from children with dengue, using RT-PCR and immunostaining. Tumor necrosis factor-alpha (TNF-alpha) and TNF-beta expression was detected in all samples by PCR and in < 50% of samples by immunostaining. Interferon-gamma (IFN-gamma) expression was detected in < 50% of samples by either method. Interleukin-2 (IL-2) and IL-4 expression was detected in a few samples by immunostaining but was not detectable by PCR. We found greater expression of TNF-alpha and IL-4 in DHF than in dengue fever or other (non-dengue) febrile illnesses. These results support the model of immunopathogenesis of DHF. However, low levels of cytokine expression in PBMC suggest that cellular activation in tissues may contribute to high serum cytokine levels in DHF.

Decreased proliferative responses to mitogens and recall Ags have been observed in PBMC obtained during several acute human viral infections. To determine whether cell-mediated responses are altered during acute dengue infection, we examined the proliferative responses of PBMC from children enrolled in a prospective study of dengue infections in Thailand. All responses of PBMC during acute illness were compared with the same patients' PBMC obtained at least 6 mo after their infection. Proliferative responses to PHA, anti-CD3, tetanus toxoid, and dengue Ags were decreased significantly in PBMC obtained during the acute infection. The proliferative responses to PHA were restored by the addition of gamma-irradiated autologous convalescent or allogeneic PBMC. Cell contact with the irradiated PBMC was necessary to restore proliferation. Non-T cells from the acute PBMC of dengue patients did not support proliferation of T cells from control donors in response to PHA, but T cells from the PBMC of patients with acute dengue proliferated if accessory cells from a control donor were present. Addition of anti-CD28 Abs restored anti-CD3-induced proliferation of the PBMC of some patients. The percentage of monocytes was reduced in the acute sample of PBMC of the dengue patients. Addition of IL-2 or IL-7, but not IL-4 or IL-12, also restored proliferation of acute PBMC stimulated with anti-CD3. The results demonstrate that both quantitative and qualitative defects in the accessory cell population during acute dengue illness result in a depression of in vitro T cell proliferation.

Eighty-five paediatric patients in Thailand with acute Japanese encephalitis (JE) were studied in 1987-99 to determine risk factors present at hospital admission which were associated with severe disease. On univariate analysis, the following factors on admission were significantly associated with the combined end-point of death or a severe neurological deficit: depressed level of consciousness, elevated concentration of cerebrospinal fluid (CSF) protein, low levels of serum and CSF IgG antibody against Japanese encephalitis virus (JEV), low level of serum IgM antibody against JEV, and a serological response consistent with primary flavivirus infection. On multivariate analysis, an initial serum anti-JEV IgM < 150 U and the absence of a prior flavivirus infection, presumably dengue, remained independent risk factors for death or a severe neurological deficit. The ability to mount an early and vigorous JEV-reactive antibody response is associated with a better outcome from acute JE. An anamnestic, anti-flavivirus, immune response induced by a prior dengue virus infection can be an important means of providing this protection.

In this study, a novel fast terminal sliding mode control technique based on the disturbance observer is recommended for the stabilization of underactuated robotic systems. The finite time disturbance observer is employed to estimate the exterior disturbances of the system and develop the finite time control law. The proposed controller can regulate the state trajectories of the underactuated systems to the origin within a finite time in the existence of external disturbances. The stability analysis of the proposed control scheme is verified via the Lyapunov stabilization theory. The designed control law is enough to drive a switching surface achieving the fast terminal sliding mode against severe model nonlinearities with large parametric uncertainties and external disturbances. Illustrative simulation results and experimental validations on a cart-inverted pendulum system are provided to display the success and efficacy of the offered method.

Using deep learning semantic segmentation for land use extraction is the most challenging problem in medium spatial resolution imagery. This is because of the deep convolution layer and multiple levels of deep steps of the baseline network, which can cause a degradation problem in small land use features. In this paper, a deep learning semantic segmentation algorithm which comprises an adjustment network architecture (LoopNet) and land use dataset is proposed for automatic land use classification using Landsat 8 imagery. The experimental results illustrate that deep learning semantic segmentation using the baseline network (SegNet, U-Net) outperforms pixel-based machine learning algorithms (MLE, SVM, RF) for land use classification. Furthermore, the LoopNet network, which comprises a convolutional loop and convolutional block, is superior to other baseline networks (SegNet, U-Net, PSPnet) and improvement networks (ResU-Net, DeeplabV3+, U-Net++), with 89.84% overall accuracy and good segmentation results. The evaluation of multispectral bands in the land use dataset demonstrates that Band 5 has good performance in terms of extraction accuracy, with 83.91% overall accuracy. Furthermore, the combination of different spectral bands (Band 1–Band 7) achieved the highest accuracy result (89.84%) compared to individual bands. These results indicate the effectiveness of LoopNet and multispectral bands for land use classification using Landsat 8 imagery.

OBJECTIVE: To determine the temporal intervals at which spatial clustering of dengue hospitalisations occurs. METHODS: Space-time analysis of 262 people hospitalised and serologically confirmed with dengue virus infections in Kamphaeng Phet, Thailand was performed. The cases were observed between 1 January 2009 and 6 May 2011. Spatial coordinates of each patient's home were captured using the Global Positioning System. A novel method based on the Knox test was used to determine the temporal intervals between cases at which spatial clustering occurred. These intervals are indicative of the length of time between successive illnesses in the chain of dengue virus transmission. RESULTS: The strongest spatial clustering occurred at the 15-17-day interval. There was also significant spatial clustering over short intervals (2-5 days). The highest excess risk was observed within 200 m of a previous hospitalised case and significantly elevated risk persisted within this distance for 32-34 days. CONCLUSIONS: Fifteen to seventeen days are the most likely serial interval between successive dengue illnesses. This novel method relies only on passively detected, hospitalised case data with household locations and provides a useful tool for understanding region-specific and outbreak-specific dengue virus transmission dynamics.

Saliva was collected prospectively from patients presenting with suspected dengue infection 4 to 8 days after the onset of symptoms and assayed by a commercial dengue immunoglobulin M (IgM) and IgG capture enzyme-linked immunosorbent assay (ELISA) (PanBio Dengue Duo ELISA). Laboratory diagnosis was based on virus isolation and on hemagglutination inhibition (HAI) assay and an in-house IgM and IgG capture ELISA. With a positive result defined as either salivary IgM or IgG levels above the cutoff value, an overall sensitivity of 92% was obtained for both primary- and secondary-dengue patients (22 of 24), while no patients with non-flavivirus infections (n = 11) and no healthy laboratory donors (n = 17) showed elevation of salivary antidengue antibody (100% specificity). Salivary IgG levels correlated well with serum HAI titer (r = 0.78), and salivary IgG levels could be used to distinguish between primary- and secondary-dengue virus infections.

Designing the instructional model with the innovative the Research-Based Learning Strategy Lesson Plans of the effectiveness of the processing performance and the resulting performance (E1/E2) with the IOC value determining standardized criteria of 80/80 were developed. Students&rsquo; perceptions were assessed with the 30-item Research-Based Learning Strategies (RBLS) in six scales. Students&rsquo; creative thinking abilities adapted version into the 10-item Creative Thinking Ability Questionnaire (CTAQ) was used. Associations between students&rsquo; perceptions of their cultivating learning strategies and their creative thinking abilities were analyzed. A sample size consisted of 626 senior teacher students from 21 Foundational Education classes in Kamphaeng Phet Rajabhat University in two semesters of the academic year 2015. Statistically significant was found that; the lessening effectiveness (E1/E2) evidenced of 80.73/80.98 over the threshold setting was 80/80. The 5-professional expert educators&rsquo; responses of the IOC revealed of 0.76. The RBLS and CTAQ were validated and reliable with the internal consistency was analyzed. Students&rsquo; perceptions of their RBLS and their CTAQ toward Foundational Education Subject were positive relatively. The multiple correlations R was 0.79 and the predictive efficiency value (R2) indicated that 62% of the variances in students&rsquo; creative thinking abilities to their classes were attributable for their perceptions of their individualized classroom environments. Suggestions that of these research findings, the designing instructional model for fostering students&rsquo; creative thinking abilities with the research methodology, research procedure, sample design of research instruments and other components of this research, it would be valuable and had the highest benefit for further research study. Key words: Designing instructional model, cultivation indoctrination, research-based learning strategy, teacher students,creative thinking ability.

Green soybean (Glycine max L.) pods (GSP) are agro-industrial waste from the production of frozen green soybean and milk. These pods contain natural antioxidants and various bioactive compounds that are still underutilized. Polyphenols and flavonoids in GSP were extracted by ultrasound technique and used in the antioxidant fortification of green soybean milk. The ultrasound extraction that yielded the highest total polyphenol content and antioxidant activities was 50% amplitude for 10 min. Response surface methodology was applied to analyze an optimum ultrasonic-assisted extraction (UAE) condition of these variables. The highest desirability was found to be 50% amplitude with an extraction time of 10.5 min. Under these conditions, the experimental total phenolic content, total flavonoid content, and antioxidant activity were well matched with the predicted values (R2 &gt; 0.70). Fortification of the GSP extracts (1–3% v/v) in green soybean milk resulted in higher levels of bioactive compounds and antioxidant activity in a dose-dependent manner. Procyanidins were found to be the main polyphenols in dried GSP crude extracts, which were present at a concentration of 0.72 ± 0.01 mg/100 g. The addition of GSP extracts obtained by using an ultrasound technique to green soybean milk increased its bioactive compound content, especially procyanidins, as well as its antioxidant activity.

A good content-based image retrieval must provide a robustness to the spatial changing problem of an image which causes from different acquisition, such as, zooming image, same image but different color, etc. These mentioned problems is the main cause to degrade the retrieval performance. To achieve the best retrieval result, an optimal feature must be invented to represent an image semantic. At present, instead of using single feature, most image retrieval system utilizes combining features between two low-level features in an image to retain high precision when faces to the mentioned problem. However, the retrieval error still remains. It causes from the combination of two unsuitable features. Thus, choosing two compatible features is another interested research issue in this area. This paper proposed a new feature combination between color correlograms and edge direction histogram (EDH) in order to give precedence to spatial information in an image. Using color correlogram will treat information about spatial color correlation, while EDH provides the geometry information in the case of the same image but different color. Performance evaluation performs by simple calculation like Euclidean Distance between the query image and image in the database. The experimental result shows that using this combining feature yields a good result while compares with the other combining scheme.

Zeolite was successfully modified by conventional synthetic route. Polyethylene glycol was employed for surface modification of zeolite. The surface of zeolite exhibited therefore hydrophobic properties. Less than 5 wt% of modified zeolites with uniform size and shape were integrated into polypropylene matrix. Mechanical properties of composite exhibited the similar trend compare to neat polypropylene. Oxygen transmission rate and water vapor transmission rate were evaluated and it exhibited the strong potential to be a good candidate material in active packaging. Keywords: Zeolite, Composite, Polypropylene, Packaging, PEG-template

Abstract Aim We conducted a prospective cohort study to evaluate the effectiveness of an integrated care model on delaying chronic kidney disease (CKD) progression in routine clinical practice in rural primary care setting. Methods After enrolment, patients with stages 3 to 4 CKD patients from five district hospitals in a northern province of Thailand (400 km from Bangkok) received integrated care comprising hospital multidisciplinary care and home visits by community care teams. Clinical characteristics and biochemical data were collected at baseline and every 3‐month interval thereafter for 36 months. The primary outcome was the rate of estimated glomerular filtration rate (eGFR) decline. Results Nine hundred and fourteen stage −3 and − 4 CKD patients were enrolled. The mean age of our cohort was 62 years. Diabetic kidney disease (DKD) was the main cause of CKD (53%) whereas hypertension was the most common co‐morbidity (92%). The mean rate of eGFR decline was −0.92 mL/min/1.73 m 2 /year. The rate of eGFR decline among patients with DKD was about three times faster than patients without DKD. Patients with higher blood pressure, metabolic acidosis, proteinuria or anaemia had a faster rate of eGFR decline. Conclusion This integrated care model at the community level was effective in delaying CKD progression in routine clinical practice situation.

The objective of this study was to determine the in vitro activities such as antioxidant and inhibitions of angiotensin converting enzyme, dipeptidyl peptidase-IV, prolyl oligopeptidase, and 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase of sacha inchi protein hydrolysate (SPH) and its membrane ultrafiltration peptide fractions. SPH was prepared after hydrolysis of sacha inchi protein using papain followed by separation into peptide fractions (F1: <1 kDa, F2: 1–3 kDa, F3: 3–5 kDa, and F4: 5–10 kDa) via ultrafiltration membranes. SPH and the peptide fractions were tested for multifunctional properties, specifically functional ability as antioxidants and enzyme inhibitors. Surface hydrophobicity was an important contributing factor to the activity of antioxidative peptides. The DPPH inhibitory activity of F4 was significantly higher (p < .05) than activities of the SPH and other fractions. The smaller peptides with <1 kDa size (F1) showed the most potent (p < .05) antioxidant properties based on the stronger scavenging of ABTS, DPPH, and superoxide radicals in addition to better attenuation of linoleic acid peroxidation. Moreover, the F1 was also the strongest inhibitor of angiotensin converting enzyme, dipeptidyl peptidase-IV, prolyl oligopeptidase inhibition, and 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase based on the lower IC50 values. It was concluded that the smaller size of the F1 peptides was the main determinant of its strong antioxidant and enzyme inhibition potency, which could be taken as an advantage to formulate functional foods and nutraceuticals with potential activities in ameliorating some of the chronic human diseases. Practical applications The results of present study indicate that SPH and its ultrafiltration fractions are potential sources of antihypertensive, antidiabetic, inhibition of POP, reduced cholesterol, and strong antioxidant peptides. The strong angiotensin converting enzyme, dipeptidyl peptidase-IV, prolyl oligopeptidase inhibition, and 3-hydroxy-3-methyl-glutaryl-coenzyme inhibitory efficiency of the F1 peptides (MW < 1 kDa) suggest potential utility as an antihypertensive, antidiabetic agent, reduce cholesterol and brain plasticity and memory formation because the small peptide size could enhance absorption from the gastrointestinal tract. Overall, results from this study indicate that SPH, especially the F1 peptides may have applications as ingredients for the formulation of functional foods and nutraceuticals.

Photovoltaic–thermal (PVT) applications have been widely studied in recent years, though commercialisation has become critical due to their operational characteristics and size. In this study, a portable PVT system was developed for mobilisation with assistance from an organic phase-change material (PCM). Two different PCM composites were developed using the PCM with charcoal (PCM + C) and charcoal and metal flakes (PCM + C + M). Considering the portability of the PVT system, conventional metal-container-based PCM storage units were avoided, and the shape-stabilised PCMs (SS-PCMs) were fitted directly on the back surface of the PV module. Further, a serpentine copper tube was placed on the SS-PCMs to extract heat energy for hot water applications. It was found that PVPCM+C+M exhibited a higher cooling rate, with peak reductions of 24.82 °C and 4.19 °C compared to the PVnoPCM and PVPCM+C, respectively. However, PVPCM+C exhibited a higher outlet water temperature difference of 11.62 °C. Secondly, an increase of more than 0.2 litres per minute showed a declining trend in cooling in the PV module. Considering the primary concern of electrical power generation, it was concluded that PVPCM+C+M is suitable for PVT mobilisation applications, owing to it having shown the highest thermal cooling per 190 g of PCM and a 1-Watt (TCPW) cooling effect of 2.482 °C. In comparison, PVPCM+C achieved a TCPW cooling effect of 1.399 °C.