Lebanese University

Correction: Volume: 292 Pages: 160-162 DOI: 10.1016/j.atherosclerosis.2019.11.020 Published: JAN 2020

Metastasis is the hallmark of cancer that is responsible for the greatest number of cancer-related deaths. Yet, it remains poorly understood. The continuous evolution of cancer biology research and the emergence of new paradigms in the study of metastasis have revealed some of the molecular underpinnings of this dissemination process. The invading tumor cell, on its way to the target site, interacts with other proteins and cells. Recognition of these interactions improved the understanding of some of the biological principles of the metastatic cell that govern its mobility and plasticity. Communication with the tumor microenvironment allows invading cancer cells to overcome stromal challenges, settle, and colonize. These characteristics of cancer cells are driven by genetic and epigenetic modifications within the tumor cell itself and its microenvironment. Establishing the biological mechanisms of the metastatic process is crucial in finding open therapeutic windows for successful interventions. In this review, the authors explore the recent advancements in the field of metastasis and highlight the latest insights that contribute to shaping this hallmark of cancer.

Game theoretical techniques have recently become prevalent in many engineering applications, notably in communications. With the emergence of cooperation as a new communication paradigm, and the need for self-organizing, decentralized, and autonomic networks, it has become imperative to seek suitable game theoretical tools that allow to analyze and study the behavior and interactions of the nodes in future communication networks. In this context, this tutorial introduces the concepts of cooperative game theory, namely coalitional games, and their potential applications in communication and wireless networks. For this purpose, we classify coalitional games into three categories: canonical coalitional games, coalition formation games, and coalitional graph games. This new classification represents an application-oriented approach for understanding and analyzing coalitional games. For each class of coalitional games, we present the fundamental components, introduce the key properties, mathematical techniques, solution concepts, and describe the methodologies for applying these games in several applications drawn from the state-of-theart research in communications. In a nutshell, this article constitutes a unified treatment of coalitional game theory tailored to the demands of communications and network engineers.

With the rapid increase of sequenced metazoan mitochondrial genomes, a detailed manual annotation is becoming more and more infeasible. While it is easy to identify the approximate location of protein-coding genes within mitogenomes, the peculiar processing of mitochondrial transcripts, however, makes the determination of precise gene boundaries a surprisingly difficult problem. We have analyzed the properties of annotated start and stop codon positions in detail, and use the inferred patterns to devise a new method for predicting gene boundaries in de novo annotations. Our method benefits from empirically observed prevalances of start/stop codons and gene lengths, and considers the dependence of these features on variations of genetic codes. Albeit not being perfect, our new approach yields a drastic improvement in the accuracy of gene boundaries and upgrades the mitochondrial genome annotation server MITOS to an even more sophisticated tool for fully automatic annotation of metazoan mitochondrial genomes.

BACKGROUND: There are few cross-national comparisons of the rates of suicide ideation and attempts across diverse countries. Nine independently conducted epidemiological surveys using similar diagnostic assessment and criteria provided an opportunity to obtain that data. METHODS: Suicide ideation and attempts were assessed on the Diagnostic Interview Schedule in over 40000 subjects drawn from the United States, Canada, Puerto Rico, France, West Germany, Lebanon, Taiwan, Korea and New Zealand. RESULTS: The lifetime prevalence rates/100 for suicide ideation ranged from 2.09 (Beirut) to 18.51 (Christchurch, New Zealand). Lifetime prevalence rates/100 for suicide attempts ranged from 0.72 (Beirut) to 5.93 (Puerto Rico). Females as compared to males had only marginally higher rates of suicidal ideation in most countries, reaching a two-fold increase in Taiwan. Females as compared to males had more consistently higher rates for suicide attempts, reaching a two- to three-fold increase in most countries. Suicide ideation and attempts in most countries were associated with being currently divorced/separated as compared to currently married. CONCLUSIONS: While the rates of suicide ideation varied widely by country, the rates of suicide attempts were more consistent across most countries. The variations were only partly explained by variation in rates of psychiatric disorders, divorce or separation among countries and are probably due to cultural features that we do not, as yet, understand.

), a calcium phosphate biomaterial, is a very promising candidate for the treatment of air, water and soil pollution. Indeed, hydroxyapatite (Hap) can be extremely useful in the field of environmental management, due in one part to its particular structure and attractive properties, such as its great adsorption capacities, its acid-base adjustability, its ion-exchange capability and its good thermal stability. Moreover, Hap is able to constitute a valuable resource recovery route. The first part of this review will be dedicated towards presenting Hap's structure and defining properties that result in its viability as an environmental remediation material. The second will focus on its use as adsorbent for wastewater and soil treatment, while indicating the mechanisms involved in this remediation process. Finally, the last part will impart all findings on Hap's applications in the field of catalysis, whether it be as catalyst, as photocatalyst, or as active phase support. Hence, all of the above will have served in showcasing the benefits gained by employing hydroxyapatite in air, water and soil clean-up.

Microplastic pollution of water and ecosystem is attracting continued attention worldwide. Due to their small sizes (≤5 mm) microplastic particles can be discharged to the environment from treated wastewater effluents. As microplastics have polluted most of our aquatic ecosystems, often finding its way into drinking water, there is urgent need to find new solutions for tackling the menace of microplastic pollution. In this work, sustainable green photocatalytic removal of microplastics from water activated by visible light is proposed as a tool for the removal of microplastics from water. We propose a novel strategy for the elimination of microplastics using glass fiber substrates to trap low density microplastic particles such as polypropylene (PP) which in parallel support the photocatalyst material. Photocatalytic degradation of PP microplastics spherical particles suspended in water by visible light irradiation of zinc oxide nanorods (ZnO NRs) immobilized onto glass fibers substrates in a flow through system is demonstrated. Upon irradiation of PP microplastics for two weeks under visible light reduced led to a reduction of the average particle volume by 65%. The major photodegradation by-products were identified using GC/MS and found to be molecules that are considered to be mostly nontoxic in the literature.

The COVID-19 pandemic has caused a complementary infodemic, whereby various outlets and digital media portals shared false information and unsourced recommendations on health. In addition, journals and authors published a mass of academic articles at a speed that suggests a non-existent or a non-rigorous peer review process. Such lapses can promote false information and adoption of health policies based on misleading data. Reliable information is vital for designing and implementing preventive measures and promoting health awareness in the fight against COVID-19. In the age of social media, information travels wide and fast, emphasizing a need for accurate data to be corroborated swiftly and for preventing misleading information from wide dissemination. Here, we discuss the implications of the COVID-19 infodemic and explore practical ways to leverage health communication strategies to overcome it. We propose the "Infodemic Response Checklist" as a comprehensive tool to overcome the challenges posed by the current and any future infodemics.

Antibiotic resistance, and, in a broader perspective, antimicrobial resistance (AMR), continues to evolve and spread beyond all boundaries. As a result, infectious diseases have become more challenging or even impossible to treat, leading to an increase in morbidity and mortality. Despite the failure of conventional, traditional antimicrobial therapy, in the past two decades, no novel class of antibiotics has been introduced. Consequently, several novel alternative strategies to combat these (multi-) drug-resistant infectious microorganisms have been identified. The purpose of this review is to gather and consider the strategies that are being applied or proposed as potential alternatives to traditional antibiotics. These strategies include combination therapy, techniques that target the enzymes or proteins responsible for antimicrobial resistance, resistant bacteria, drug delivery systems, physicochemical methods, and unconventional techniques, including the CRISPR-Cas system. These alternative strategies may have the potential to change the treatment of multi-drug-resistant pathogens in human clinical settings.

The traditional two-dimensional (2D) in vitro cell culture system (on a flat support) has long been used in cancer research. However, this system cannot be fully translated into clinical trials to ideally represent physiological conditions. This culture cannot mimic the natural tumor microenvironment due to the lack of cellular communication (cell-cell) and interaction (cell-cell and cell-matrix). To overcome these limitations, three-dimensional (3D) culture systems are increasingly developed in research and have become essential for tumor research, tissue engineering, and basic biology research. 3D culture has received much attention in the field of biomedicine due to its ability to mimic tissue structure and function. The 3D matrix presents a highly dynamic framework where its components are deposited, degraded, or modified to delineate functions and provide a platform where cells attach to perform their specific functions, including adhesion, proliferation, communication, and apoptosis. So far, various types of models belong to this culture: either the culture based on natural or synthetic adherent matrices used to design 3D scaffolds as biomaterials to form a 3D matrix or based on non-adherent and/or matrix-free matrices to form the spheroids. In this review, we first summarize a comparison between 2D and 3D cultures. Then, we focus on the different components of the natural extracellular matrix that can be used as supports in 3D culture. Then we detail different types of natural supports such as matrigel, hydrogels, hard supports, and different synthetic strategies of 3D matrices such as lyophilization, electrospiding, stereolithography, microfluid by citing the advantages and disadvantages of each of them. Finally, we summarize the different methods of generating normal and tumor spheroids, citing their respective advantages and disadvantages in order to obtain an ideal 3D model (matrix) that retains the following characteristics: better biocompatibility, good mechanical properties corresponding to the tumor tissue, degradability, controllable microstructure and chemical components like the tumor tissue, favorable nutrient exchange and easy separation of the cells from the matrix.

Single molecule FISH (smFISH) allows studying transcription and RNA localization by imaging individual mRNAs in single cells. We present smiFISH (single molecule inexpensive FISH), an easy to use and flexible RNA visualization and quantification approach that uses unlabelled primary probes and a fluorescently labelled secondary detector oligonucleotide. The gene-specific probes are unlabelled and can therefore be synthesized at low cost, thus allowing to use more probes per mRNA resulting in a substantial increase in detection efficiency. smiFISH is also flexible since differently labelled secondary detector probes can be used with the same primary probes. We demonstrate that this flexibility allows multicolor labelling without the need to synthesize new probe sets. We further demonstrate that the use of a specific acrydite detector oligonucleotide allows smiFISH to be combined with expansion microscopy, enabling the resolution of transcripts in 3D below the diffraction limit on a standard microscope. Lastly, we provide improved, fully automated software tools from probe-design to quantitative analysis of smFISH images. In short, we provide a complete workflow to obtain automatically counts of individual RNA molecules in single cells.

Data on comprehensive population-based surveillance of antimicrobial resistance is lacking. In low- and middle-income countries, the challenges are high due to weak laboratory capacity, poor health systems governance, lack of health information systems, and limited resources. Developing countries struggle with political and social dilemma, and bear a high health and economic burden of communicable diseases. Available data are fragmented and lack representativeness which limits their use to advice health policy makers and orientate the efficient allocation of funding and financial resources on programs to mitigate resistance. Low-quality data means soaring rates of antimicrobial resistance and the inability to track and map the spread of resistance, detect early outbreaks, and set national health policy to tackle resistance. Here, we review the barriers and limitations of conducting effective antimicrobial resistance surveillance, and we highlight multiple incremental approaches that may offer opportunities to strengthen population-based surveillance if tailored to the context of each country.

BACKGROUND: E-learning has been adopted internationally as the alternative teaching or learning strategy during the coronavirus disease 2019 (COVID-19) quarantine to fill the academic gap that has been created by the existing reality of the pandemic due to nationwide closures. AIM: This study aims at evaluating the prevalence of depression, anxiety, and stress symptomatology among Lebanese University Students during the COVID-19 Quarantine. METHODOLOGY: A quantitative cross sectional research design, where 520 undergraduate university students were surveyed regarding their satisfaction with e-learning and the prevalence of depression, anxiety, and stress symptomatology using depression, anxiety, and stress scale-21 elements. RESULTS: Learning through online platforms have given rise to depression and anxiety disorders among undergraduate university students, where there was a significant correlation between student satisfaction and prevalence of depression, anxiety, and stress. CONCLUSION: The sudden shift to exclusive e-learning methods of instruction have produced anxiety and depression symptoms among a significant portion of the students due to the stressful load of work required.

O1 isolates, across 45 African countries and over a 49-year period, to show that past epidemics were attributable to a single expanded lineage. This lineage was introduced at least 11 times since 1970, into two main regions, West Africa and East/Southern Africa, causing epidemics that lasted up to 28 years. The last five introductions into Africa, all from Asia, involved multidrug-resistant sublineages that replaced antibiotic-susceptible sublineages after 2000. This phylogenetic framework describes the periodicity of lineage introduction and the stable routes of cholera spread, which should inform the rational design of control measures for cholera in Africa.

It is acknowledged that physicians do not seek the same expert aid for themselves as they would offer their patients. In their preclinical years, medical students appear to espouse comparable behavior. To many, medicine is described as a never-ending path that places the student under heavy stress and burnout from the beginning, leaving him/her vulnerable and with insufficient coping methods. Hence, the objective of this study is to 1) explore the prevalence of stress and burnout among preclinical medical students, and 2) propose solutions to decrease stress and burnout and improve medical education in the preclinical years. A detailed scholarly research strategy using Google Scholar, Scopus, Embase, MEDLINE and PubMed was implemented to highlight key themes that are relevant to preclinical medical students' stress and burnout. Stress varied among different samples of medical students and ranged between 20.9% and 90%. Conversely, burnout ranged between 27% and 75%. Methods that help in reducing the incidence of stress and burnout by promoting strategies that focus on personal engagement, extracurricular activities, positive reinterpretation and expression of emotion, student-led mentorship programs, evaluation systems, career counseling and life coaching should be adopted.

Apitherapy is an alternate therapy that relies on the usage of honeybee products, most importantly bee venom for the treatment of many human diseases. The venom can be introduced into the human body by manual injection or by direct bee stings. Bee venom contains several active molecules such as peptides and enzymes that have advantageous potential in treating inflammation and central nervous system diseases, such as Parkinson's disease, Alzheimer's disease, and amyotrophic lateral sclerosis. Moreover, bee venom has shown promising benefits against different types of cancer as well as anti-viral activity, even against the challenging human immunodeficiency virus (HIV). Many studies described biological activities of bee venom components and launched preclinical trials to improve the potential use of apitoxin and its constituents as the next generation of drugs. The aim of this review is to summarize the main compounds of bee venom, their primary biological properties, mechanisms of action, and their therapeutic values in alternative therapy strategies.

The study of fungal species evolved radically with the development of molecular techniques and produced new evidence to understand specific fungal mechanisms such as the production of toxic secondary metabolites. Taking advantage of these technologies to improve food safety, the molecular study of toxinogenic species can help elucidate the mechanisms underlying toxin production and enable the development of new effective strategies to control fungal toxicity. Numerous studies have been made on genes involved in aflatoxin B1 (AFB1) production, one of the most hazardous carcinogenic toxins for humans and animals. The current review presents the roles of these different genes and their possible impact on AFB1 production. We focus on the toxinogenic strains Aspergillus flavus and A. parasiticus, primary contaminants and major producers of AFB1 in crops. However, genetic reports on A. nidulans are also included because of the capacity of this fungus to produce sterigmatocystin, the penultimate stable metabolite during AFB1 production. The aim of this review is to provide a general overview of the AFB1 enzymatic biosynthesis pathway and its link with the genes belonging to the AFB1 cluster. It also aims to illustrate the role of global environmental factors on aflatoxin production and the recent data that demonstrate an interconnection between genes regulated by these environmental signals and aflatoxin biosynthetic pathway.

Antimicrobial peptides constitute one of the most promising alternatives to antibiotics since they could be used to treat bacterial infections, especially those caused by multidrug-resistant pathogens. Many antimicrobial peptides, with various activity spectra and mechanisms of actions, have been described. This review focuses on their use against ESKAPE bacteria, especially in biofilm treatments, their synergistic activity, and their application as prophylactic agents. Limitations and challenges restricting therapeutic applications are highlighted, and solutions for each challenge are evaluated to analyze whether antimicrobial peptides could replace antibiotics in the near future.

Low-power wide area networks (LPWANs) constitute a type of networks which is used to connect things to the Internet from a wide variety of sectors. These types of technologies provide the Internet of Things (IoT) devices with the ability to transmit few bytes of data for long ranges, taking into consideration minimum power consumption. In parallel, IoT applications will cover a wide range of human and life needs from smart environments (cities, home, transportation, etc.) to health and quality of life. Among these popular LPWANs technologies, we have identified the unlicensed frequency band (LoRa, DASH7, SigFox, Wi-SUN, etc.), and the licensed frequency band standards (NB-IoT, LTE Cat-M, EC-GSM-IoT, etc.). In general, both types of standards only consider fixed interconnected things, and less attention has been provided to the mobility of the things or devices. In this paper, we address the mobility of the things and the connectivity in each of the three LPWAN standards: LoRaWAN, DASH7, and NB-IoT. In particular, we show how the mobility of things can be achieved when transmitting and receiving data. Then, we provide a general and technical comparison for the three standards. Finally, we illustrate several application scenarios where the mobility is required, and we show how to select the most suited standard. We also discuss the research challenges and perspectives.

Abstract Environmental and energy problems have drawn much attention owing to rapid population growth and accelerated economic development. For instance, photocatalysis, “a green technology”, plays an important role in solar‐energy conversion owing to its potential to solve energy and environmental problems. Recently, many efforts have been devoted to improving visible‐light photocatalytic activity by using titanium dioxide as a photocatalyst as a result of its wide range of applications in the energy and environment fields. However, fast charge recombination and an absorption edge in the UV range limit the photocatalytic efficiency of TiO 2 under visible‐light irradiation. Many investigations have been undertaken to overcome the limitations of TiO 2 and, therefore, to enhance its photocatalytic activity under visible light. The present literature review focuses on different strategies used to promote the separation efficiency of electron–hole pairs and to shift the absorption edge of TiO 2 to the visible region. Current synthesis techniques used to elaborate several nanostructures of TiO 2 ‐based materials, recent progress in enhancing visible photocatalytic activity, and different photocatalysis applications will be discussed. On the basis of the studies reported in the literature, we believe that this review will help in the development of new strategies to improve the visible‐light photocatalytic performance of TiO 2 ‐based materials further.