Saitama University

International audience

The Swift mission, scheduled for launch in 2004, is a multiwavelength observatory for gamma-ray burst (GRB) astronomy. It is a first-of-its-kind autonomous rapid-slewing satellite for transient astronomy and pioneers the way for future rapid-reaction and multiwavelength missions. It will be far more powerful than any previous GRB mission, observing more than 100 bursts yr -1 and performing detailed X-ray and UV/optical afterglow observations spanning timescales from 1 minute to several days after the burst. The objectives are to (1) determine the origin of GRBs, (2) classify GRBs and search for new types, (3) study the interaction of the ultrarelativistic outflows of GRBs with their surrounding medium, and (4) use GRBs to study the early universe out to z > 10. The mission is being developed by a NASA-led international collaboration. It will carry three instruments: a new-generation wide-field gamma-ray (15-150 keV) detector that will detect bursts, calculate 1'-4' positions, and trigger autonomous spacecraft slews; a narrow-field X-ray telescope that will give 5'' positions and perform spectroscopy in the 0.2-10 keV band; and a narrow-field UV/optical telescope that will operate in the 170-600 nm band and provide 0 3 positions and optical finding charts. Redshift determinations will be made for most bursts. In addition to the primary GRB science, the mission will perform a hard X-ray survey to a sensitivity of ~1 mcrab (~2 × 10 -11 ergs cm -2 s -1 in the 15-150 keV band), more than an order of magnitude better than HEAO 1 A-4. A flexible data and operations system will allow rapid follow-up observations of all types of high-energy transients, with rapid data downlink and uplink available through the NASA TDRSS system. Swift transient data will be rapidly distributed to the astronomical community, and all interested observers are encouraged to participate in follow-up measurements. A Guest Investigator program for the mission will provide funding for community involvement. Innovations from the Swift program applicable to the future include (1) a large-area gamma-ray detector using the new CdZnTe detectors, (2) an autonomous rapid-slewing spacecraft, (3) a multiwavelength payload combining optical, X-ray, and gamma-ray instruments, (4) an observing program coordinated with other ground-based and space-based observatories, and (5) immediate multiwavelength data flow to the community. The mission is currently funded for 2 yr of operations, and the spacecraft will have a lifetime to orbital decay of ~8 yr.

autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.

A new method for three-dimensional image formation is proposed in which the distribution of complex amplitude at a plane is measured by phase-shifting interferometry and then Fresnel transformed by a digital computer. The method can reconstruct an arbitrary cross section of a three-dimensional object with higher image quality and a wider viewing angle than from conventional digital holography using an off-axis configuration. Basic principles and experimental verification are described.

A concise state-of-the-art survey of fiber-reinforced polymer (also known as fiber-reinforced plastic) composites for construction applications in civil engineering is presented. The paper is organized into separate sections on structural shapes, bridge decks, internal reinforcements, externally bonded reinforcements, and standards and codes. Each section includes a historical review, the current state of the art, and future challenges.

A gravitational theory is formulated on the Weitzenb\"ock space-time, characterized by the vanishing curvature tensor (absolute parallelism) and by the torsion tensor formed of four parallel vector fields. This theory is called new general relativity, since Einstein in 1928 first gave its original form. New general relativity has three parameters ${c}_{1}$, ${c}_{2}$, and $\ensuremath{\lambda}$, besides the Einstein constant $\ensuremath{\kappa}$. In this paper we choose ${c}_{1}=0={c}_{2}$, leaving open $\ensuremath{\lambda}$. We prove, among other things, that (i) a static, spherically symmetric gravitational field is given by the Schwarzschild metric, that (ii) in the weak-field approximation an antisymmetric field of zero mass and zero spin exists, besides gravitons, and that (iii) new general relativity agrees with all the experiments so far carried out.

Class III phosphatidylinositol 3-kinase (PI3-kinase) regulates multiple membrane trafficking. In yeast, two distinct PI3-kinase complexes are known: complex I (Vps34, Vps15, Vps30/Atg6, and Atg14) is involved in autophagy, and complex II (Vps34, Vps15, Vps30/Atg6, and Vps38) functions in the vacuolar protein sorting pathway. Atg14 and Vps38 are important in inducing both complexes to exert distinct functions. In mammals, the counterparts of Vps34, Vps15, and Vps30/Atg6 have been identified as Vps34, p150, and Beclin 1, respectively. However, orthologues of Atg14 and Vps38 remain unknown. We identified putative mammalian homologues of Atg14 and Vps38. The Vps38 candidate is identical to UV irradiation resistance-associated gene (UVRAG), which has been reported as a Beclin 1-interacting protein. Although both human Atg14 and UVRAG interact with Beclin 1 and Vps34, Atg14, and UVRAG are not present in the same complex. Although Atg14 is present on autophagic isolation membranes, UVRAG primarily associates with Rab9-positive endosomes. Silencing of human Atg14 in HeLa cells suppresses autophagosome formation. The coiled-coil region of Atg14 required for binding with Vps34 and Beclin 1 is essential for autophagy. These results suggest that mammalian cells have at least two distinct class III PI3-kinase complexes, which may function in different membrane trafficking pathways.

Small, compact genomes of ultrasmall unicellular algae provide information on the basic and essential genes that support the lives of photosynthetic eukaryotes, including higher plants. Here we report the 16,520,305-base-pair sequence of the 20 chromosomes of the unicellular red alga Cyanidioschyzon merolae 10D as the first complete algal genome. We identified 5,331 genes in total, of which at least 86.3% were expressed. Unique characteristics of this genomic structure include: a lack of introns in all but 26 genes; only three copies of ribosomal DNA units that maintain the nucleolus; and two dynamin genes that are involved only in the division of mitochondria and plastids. The conserved mosaic origin of Calvin cycle enzymes in this red alga and in green plants supports the hypothesis of the existence of single primary plastid endosymbiosis. The lack of a myosin gene, in addition to the unexpressed actin gene, suggests a simpler system of cytokinesis. These results indicate that the C. merolae genome provides a model system with a simple gene composition for studying the origin, evolution and fundamental mechanisms of eukaryotic cells.

Transcription factors belonging to the APETALA2/Ethylene Responsive Factor (AP2/ERF) family are conservatively widespread in the plant kingdom. These regulatory proteins are involved in the control of primary and secondary metabolism, growth and developmental programs, as well as responses to environmental stimuli. Due to their plasticity and to the specificity of individual members of this family, AP2/ERF transcription factors represent valuable targets for genetic engineering and breeding of crops. In this review, we integrate the evidence collected from functional and structural studies to describe their different mechanisms of action and the regulatory pathways that affect their activity.

High-sensitivity wide-band X-ray spectroscopy is the key feature of the Suzaku X-ray observatory, launched on 2005 July 10. This paper summarizes the spacecraft, in-orbit performance, operations, and data processing that are related to observations. The scientific instruments, the high-throughput X-ray telescopes, X-ray CCD cameras, non-imaging hard X-ray detector are also described.

Rapid, long-distance signaling in plants A plant injured on one leaf by a nibbling insect can alert its other leaves to begin anticipatory defense responses. Working in the model plant Arabidopsis , Toyota et al. show that this systemic signal begins with the release of glutamate, which is perceived by glutamate receptor–like ion channels (see the Perspective by Muday and Brown-Harding). The ion channels then set off a cascade of changes in calcium ion concentration that propagate through the phloem vasculature and through intercellular channels called plasmodesmata. This glutamate-based long-distance signaling is rapid: Within minutes, an undamaged leaf can respond to the fate of a distant leaf. Science , this issue p. 1112 ; see also p. 1068

Ground-based gamma-ray astronomy has had a major breakthrough with the impressive results obtained using systems of imaging atmospheric Cherenkov telescopes. Ground-based gamma-ray astronomy has a huge potential in astrophysics, particle physics and cosmology. CTA is an international initiative to build the next generation instrument, with a factor of 5–10 improvement in sensitivity in the 100 GeV–10 TeV range and the extension to energies well below 100 GeV and above 100 TeV. CTA will consist of two arrays (one in the north, one in the south) for full sky coverage and will be operated as open observatory. The design of CTA is based on currently available technology. This document reports on the status and presents the major design concepts of CTA.

Activation of caspase-12 from procaspase-12 is specifically induced by insult to the endoplasmic reticulum (ER) (Nakagawa, T., Zhu, H., Morishima, N., Li, E., Xu, J., Yankner, B. A., and Yuan, J. (2000) Nature 403, 98-103), yet the functional consequences of caspase-12 activation have been unclear. We have shown that recombinant caspase-12 specifically cleaves and activates procaspase-9 in cytosolic extracts. The activated caspase-9 catalyzes cleavage of procaspase-3, which is inhibitable by a caspase-9-specific inhibitor. Although cytochrome c released from mitochondria has been believed to be required for caspase-9 activation during apoptosis (Zou, H., Henzel, W. J., Liu, X., Lutschg, A., and Wang, X. (1997) Cell 90, 405-413, Li, P., Nijhawan, D., Budihardjo, I., Srinivasula, S. M., Ahmad, M., Alnemri, E. S., and Wang, X. (1997) Cell 91, 479-489), caspase-9 as well as caspase-12 and -3 are activated in cytochrome c-free cytosols in murine myoblast cells under ER stress. These results suggest that caspase-12 can activate caspase-9 without involvement of cytochrome c. To examine the role of caspase-12 in the activation of downstream caspases, we used a caspase-12-binding protein, which we identified in a yeast two-hybrid screen, for regulation of caspase-12 activation. The binding protein protects procaspase-12 from processing in vitro. Stable expression of the binding protein renders procaspase-12 insensitive to ER stress, thereby suppressing apoptosis and the activation of caspase-9 and -3. These data suggest that procaspase-9 is a substrate of caspase-12 and that ER stress triggers a specific cascade involving caspase-12, -9, and -3 in a cytochrome c-independent manner.

NAC transcription factors are one of the largest families of transcriptional regulators in plants, and members of the NAC gene family have been suggested to play important roles in the regulation of the transcriptional reprogramming associated with plant stress responses. A phylogenetic analysis of NAC genes, with a focus on rice and Arabidopsis, was performed. Herein, we present an overview of the regulation of the stress responsive NAC SNAC/(IX) group of genes that are implicated in the resistance to different stresses. SNAC factors have important roles for the control of biotic and abiotic stresses tolerance and that their overexpression can improve stress tolerance via biotechnological approaches. We also review the recent progress in elucidating the roles of NAC transcription factors in plant biotic and abiotic stresses. Modification of the expression pattern of transcription factor genes and/or changes in their activity contribute to the elaboration of various signaling pathways and regulatory networks. However, a single NAC gene often responds to several stress factors, and their protein products may participate in the regulation of several seemingly disparate processes as negative or positive regulators. Additionally, the NAC proteins function via auto-regulation or cross-regulation is extensively found among NAC genes. These observations assist in the understanding of the complex mechanisms of signaling and transcriptional reprogramming controlled by NAC proteins.

To simulate numerically the microstructure of shear bands, the distinct element method was modified such that the effect of rolling resistance at contacts could be taken into account. To this end, the relative movement by rolling is related to the relative rotation between two particles with a common contact, which makes it possible to generalize the conservation law of angular momentum for a more general case than the moments can be transmitted through contacts. Not only the generation of large voids inside a shear band but also the high gradient of particle rotation along the shear band boundaries can be reproduced, in a manner quite similar to those of natural granular soils, when the rolling resistance at contacts is considered. The development of columns is a common feature that appears in the strain hardening process, and buckling of the columns parallels the development of shear bands. The generation of large voids and the high gradient of particle rotation are produced as a result of the buckling of the columns.

The glymphatic system is a brain-wide clearance pathway; its impairment contributes to the accumulation of amyloid-β. Influx of cerebrospinal fluid (CSF) depends upon the expression and perivascular localization of the astroglial water channel aquaporin-4 (AQP4). Prompted by a recent failure to find an effect of Aqp4 knock-out (KO) on CSF and interstitial fluid (ISF) tracer transport, five groups re-examined the importance of AQP4 in glymphatic transport. We concur that CSF influx is higher in wild-type mice than in four different Aqp4 KO lines and in one line that lacks perivascular AQP4 (Snta1 KO). Meta-analysis of all studies demonstrated a significant decrease in tracer transport in KO mice and rats compared to controls. Meta-regression indicated that anesthesia, age, and tracer delivery explain the opposing results. We also report that intrastriatal injections suppress glymphatic function. This validates the role of AQP4 and shows that glymphatic studies must avoid the use of invasive procedures.

Instability of the alternate-bar type in straight channels has long been identified as the cause of fluvial meandering. The condition of inerodible sidewalls, however, does not allow a meandering channel to develop. Herein a stability analysis of a sinuous channel with erodible banks allows for delineation of a ‘bend’ instability that does not occur in straight channels, and differs from the alternate-bar instability. In the case of alluvial meanders, the two mechanisms are shown to operate at similar characteristic wavelengths. This provides a rationale for the continuous evolution of alternate bars into true bends such that each bend contains one alternate bar. The same bend instability applies to incised meanders. A mechanism for incised alternate bars which differs from that for the alluvial case appears to operate at different characteristic wavelengths than that of bend instability. Analysis of data suggests that meandering in supraglacial meltwater streams is primarily due to the alternate bar mechanism, whereas the meandering of rills incised in cohesive material and of caves is likely due to the bend mechanism. The meander wavelength of incised reaches of meandering streams is often longer than that of adjacent alluvial reaches. An explanation is offered in terms of bend instability.

Rock masses, which commonly contain a large number of discontinuities, are treated as homogeneous, anisotropic porous media to formulate the corresponding permeability tensor. This has been successfully achieved by introducing a symmetric tensor (crack tensor) which depends only on the geometry of the related cracks (aperture, size and orientation). The principal directions associated with the symmetric crack tensor are coaxial with those of the permeability tensor. The first invariant of the crack tensor is proportional to the mean permeability, while the deviatoric part is related to the anisotropic permeability. These results are well supported by numerical experiments on the permeability of cracked media by Long, Remer, Wilson and Witherspoon. An actual rock mass (moderately jointed granite) was studied to see whether the crack tensor can be determined in situ. Stereology, based on geometrical statistics, provides a sound basis for determining the crack tensor in situ. The crack tensor is obtained by treating statistically the crack orientation data presented via a stereographic projection together with the detailed mapping of crack traces visible on rock exposures. Des masses rocheuses, qui contiennent généalement un grand nombre de discontinuités, sont traitées comme des matiéres poreuses anisotropes homogénes pour formuler le tenseur de perméabilité correspondante. Ceci a été obtenu en introduisant un tenseur symétrique (tenseur de fissure) qui ne dépend que de la géometrie des fissures associées (ouverture, grandeur et orientation). Les directions principales associées avec le tenseur symétrique des fissures sont coaxiales avec celles du tenseur de perméabilité. Le premier invariant du tenseur des fissures est proportionnel à la perméabilité moyenne, tandis que la partie déviatorique est reliée à la perméabilité anisotrope. Ces résultats sont clairement confirmés par de nombreuses expériences sur la perméabilité des matiéres fissurées effectuées par Long, Remer, Wilson et Witherspoon. Une masse rocheuse (granit modérement fracturé) a été étudiée pour découvrir si on pouvait déterminer le tenseur des fissures en place. La stérologie, basée sur la statistique géometrique, fournit une base valable pour determiner le tenseur des fissures en place. Le tenseur des fissures est obtenu par le traitement statistique des données de l'orientation des fissures présentées par projection stéréographique, accompagné du relevé detail1é des traces de fissures visibles sur les roches exposées.

The cosmic-ray energy spectrum above ${10}^{18.5}\mathrm{eV}$ is reported using the updated data set of the Akeno Giant Air Shower Array from February 1990 to October 1997. The energy spectrum extends beyond ${10}^{20}\mathrm{eV}$ and the energy gap between the highest energy event and the others is being filled up with recently observed events. The spectral shape suggests the absence of the 2.7 K cutoff in the energy spectrum or a possible presence of a new component beyond the 2.7 K cutoff.

Improvements both in accuracy and speed are described for the technique of measuring the microwave dielectric properties of low-loss materials by using a dielectric rod resonator short-circuited at both ends by two parallel conducting plates. A technique for measuring the effective surface resistance R/sub s/ of the conducting plates is proposed to allow the accurate measurement of the loss tangent tan delta. By means of the first-order approximation, the expressions are analytically derived for estimating the errors of the measured values of relative permittivity epsilon/sub r/, tan delta, and R/sub r/, for measuring the temperature coefficient of epsilon/sub r/, and for determining the required size of the conducting plates. Computer-aided measurements are realized by using these expressions. It is shown that the temperature dependence of R/sub s/, should be considered in the tan delta measurement. The copper plates used in this experiment have the relative conductivity of 91.0+-2.7 percent at 20°C, estimated from the measured R/sub s/ value. For a 99.9-percent alumina ceramic rod sample, the results measured at 7.69 GHz and 25°C show that epsilon/sub r/,= 9.687+-0.003 and tan delta = (1.6+-0.2)x 10/sup -5/. The temperature coefficients measured between 25 and 100°C are 112x10/sup -6//°C for epsilon/sub r/, and 23x10/sup -4//°C for tan delta.