Universidade Federal do Pampa

We report on the micro-Raman spectroscopy of monolayer, bilayer, trilayer, and many layers of graphene (graphite) bombarded by low-energy argon ions with different doses. The evolution of peak frequencies, intensities, linewidths, and areas of the main Raman bands of graphene is analyzed as function of the distance between defects and number of layers. We describe the disorder-induced frequency shifts and the increase in the linewidth of the Raman bands by means of a spatial-correlation model. Also, the evolution of the relative areas ${A}_{D}/{A}_{G}$, ${A}_{{D}^{\ensuremath{'}}}/{A}_{G}$, and ${A}_{{G}^{\ensuremath{'}}}/{A}_{G}$ is described by a phenomenological model. The present results can be used to fully characterize disorder in graphene systems.

Additive main effect and multiplicative interaction (AMMI) and best linear unbiased prediction (BLUP) are popular methods for analyzing multi‐environment trials (MET). The AMMI has nice graphical tools for modeling genotype‐vs.‐environment interaction (GEI) but fails in some aspects, such as accommodating a linear mixed‐effect model (LMM) structure. The BLUP provides reliable estimates but new insights to deal graphically with a random GEI structure are needed. This article compares the predictive success of BLUP and AMMI, shows how to generate biplots for modeling GEI in MET analysis using LMM, and proposes a new quantitative genotypic stability measure called WAASB, which is the W eighted A verage of A bsolute S cores from the singular value decomposition of the matrix of BLUPs for the GEI effects generated by an LMM. We also introduced the theoretical basis of a superiority index that allows weighting between mean performance and stability, which was conveniently called WAASBY. The B LUP was found to outperform AMMI in the analysis of four real MET trials. The application of our indexes is illustrated using an oat ( Avena sativa L.) MET dataset. It was shown that reliable measures of stability using WAASB may help breeders and agronomists to make correct decisions when selecting or recommending genotypes. In addition, the simultaneous selection index, WAASBY, will be useful when the selection should consider different weights for stability and mean performance. Some advantages over existing statistics are discussed. Finally, the implementation of the procedures of this article in future studies is facilitated by an R package containing all required functions. Core Ideas The predictive accuracy of BLUP and AMMI was investigated using four real datasets. BLUP was found to outperform AMMI in all datasets analyzed. A genotypic stability index that inherits the principles of AMMI and BLUP was proposed. A superiority index that allows weighting between mean performance and stability was proposed. An R package with useful functions for MET analysis is presented.

Environmental contamination has exposed humans to various metal agents, including mercury. This exposure is more common than expected, and the health consequences of such exposure remain unclear. For many years, mercury was used in a wide variety of human activities, and now, exposure to this metal from both natural and artificial sources is significantly increasing. Many studies show that high exposure to mercury induces changes in the central nervous system, potentially resulting in irritability, fatigue, behavioral changes, tremors, headaches, hearing and cognitive loss, dysarthria, incoordination, hallucinations, and death. In the cardiovascular system, mercury induces hypertension in humans and animals that has wide-ranging consequences, including alterations in endothelial function. The results described in this paper indicate that mercury exposure, even at low doses, affects endothelial and cardiovascular function. As a result, the reference values defining the limits for the absence of danger should be reduced.

Over the last decades, the study of extremophiles has providing ground breaking discoveries that challenge the paradigms of modern biology and make us rethink intriguing questions such as "what is life?", "what are the limits of life?", and "what are the fundamental features of life?". These findings and possibilities have made the study of life in extreme environments one of the most exciting areas of research in recent decades. However, despite the latest advances we are just in the beginning of exploring and characterizing the world of extremophiles. This special issue discusses several aspects of these fascinating organisms, exploring their habitats, biodiversity, ecology, evolution, genetics, biochemistry, and biotechnological applications in a collection of exciting reviews and original articles written by leading experts and research groups in the field. [...].

Manganese (Mn) is an essential heavy metal. However, Mn's nutritional aspects are paralleled by its role as a neurotoxicant upon excessive exposure. In this review, we covered recent advances in identifying mechanisms of Mn uptake and its molecular actions in the brain as well as promising neuroprotective strategies. The authors focused on reporting findings regarding Mn transport mechanisms, Mn effects on cholinergic system, behavioral alterations induced by Mn exposure and studies of neuroprotective strategies against Mn intoxication. We report that exposure to Mn may arise from environmental sources, occupational settings, food, total parenteral nutrition (TPN), methcathinone drug abuse or even genetic factors, such as mutation in the transporter SLC30A10. Accumulation of Mn occurs mainly in the basal ganglia and leads to a syndrome called manganism, whose symptoms of cognitive dysfunction and motor impairment resemble Parkinson's disease (PD). Various neurotransmitter systems may be impaired due to Mn, especially dopaminergic, but also cholinergic and GABAergic. Several proteins have been identified to transport Mn, including divalent metal tranporter-1 (DMT-1), SLC30A10, transferrin and ferroportin and allow its accumulation in the central nervous system. Parallel to identification of Mn neurotoxic properties, neuroprotective strategies have been reported, and these include endogenous antioxidants (for instance, vitamin E), plant extracts (complex mixtures containing polyphenols and non-characterized components), iron chelating agents, precursors of glutathione (GSH), and synthetic compounds that can experimentally afford protection against Mn-induced neurotoxicity.

This paper describes the design and implementation of a discrete controller for grid-connected voltage-source inverters with an <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">LCL</i> filter usually found in wind power generation systems. First, a theorem that relates the controllability of the discrete dynamic equation of the inverter with <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">LCL</i> filter and the sampling frequency is derived. Then, a condition to obtain a partial state feedback controller robust to grid impedance uncertainties and based on linear matrix inequalities is proposed. This controller guarantees the stability and damping of the <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">LCL</i> filter resonance for a large set of grid conditions without requiring self-tuning procedures. Finally, an internal model controller is added to ensure asymptotic reference tracking and disturbance rejection, significantly reducing the impact of grid background voltage distortion on the output currents. Experimental results are presented to support the theoretical analysis and to demonstrate the system performance.

Microbes have a central role in soil global biogeochemical process, yet specific microbe-microbe relationships are largely unknown. Analytical approaches as network analysis may shed new lights in understanding of microbial ecology and environmental microbiology. We investigated the soil bacterial community interactions through cultivation-independent methods in several land uses common in two Brazilian biomes. Using correlation network analysis we identified bacterial genera that presented important microbial associations within the soil community. The associations revealed nonrandomly structured microbial communities and clusters of operational taxonomic units (OTUs) that reflected relevant bacterial relationships. Possible keystone genera were found in each soil. Irrespective of the biome or land use studied only a small portion of OTUs showed positive or negative interaction with other members of the soil bacterial community. The more interactive genera were also more abundant however, within those genera, the abundance was not related to taxon importance as measured by the Betweenness Centrality. Most of the soil bacterial genera were important to the overall connectance of the network, whereas only few genera play a key role as connectors, mainly belonged to phyla Proteobacteria and Actinobacteria. Finally it was observed that each land use presented a different set of keystone genera and that no keystone genus presented a generalized distribution. Taking into account that species interactions could be more important to soil processes than species richness and abundance, especially in complex ecosystems, this approach might represent a step forward in microbial ecology beyond the conventional studies of microbial richness and abundance.

The coronavirus disease 2019 (COVID-19) pandemic is altering dynamics in academia, and people juggling remote work and domestic demands - including childcare - have felt impacts on their productivity. Female authors have faced a decrease in paper submission rates since the beginning of the pandemic period. The reasons for this decline in women's productivity need to be further investigated. Here, we analyzed the influence of gender, parenthood and race on academic productivity during the pandemic period based on a survey answered by 3,345 Brazilian academics from various knowledge areas and research institutions. Productivity was assessed by the ability to submit papers as planned and to meet deadlines during the initial period of social isolation in Brazil. The findings revealed that male academics - especially those without children - are the least affected group, whereas Black women and mothers are the most impacted groups. These impacts are likely a consequence of the well-known unequal division of domestic labor between men and women, which has been exacerbated during the pandemic. Additionally, our results highlight that racism strongly persists in academia, especially against Black women. The pandemic will have long-term effects on the career progression of the most affected groups. The results presented here are crucial for the development of actions and policies that aim to avoid further deepening the gender gap in academia.

Abstract The area used for mineral extraction is a key indicator for understanding and mitigating the environmental impacts caused by the extractive sector. To date, worldwide data products on mineral extraction do not report the area used by mining activities. In this paper, we contribute to filling this gap by presenting a new data set of mining extents derived by visual interpretation of satellite images. We delineated mining areas within a 10 km buffer from the approximate geographical coordinates of more than six thousand active mining sites across the globe. The result is a global-scale data set consisting of 21,060 polygons that add up to 57,277 km 2 . The polygons cover all mining above-ground features that could be identified from the satellite images, including open cuts, tailings dams, waste rock dumps, water ponds, and processing infrastructure. The data set is available for download from https://doi.org/10.1594/PANGAEA.910894 and visualization at www.fineprint.global/viewer .

Biodiversity is one of the most fundamental properties of Nature. It underpins the stability of ecosystems, provides vast bioresources for economic use, and has important cultural significance for many people. The Pampa biome, located in the southernmost state of Brazil, Rio Grande do Sul, illustrates the direct and indirect interdependence of humans and biodiversity. The Brazilian Pampa lies within the South Temperate Zone where grasslands scattered with shrubs and trees are the dominant vegetation. The soil, originating from sedimentary rocks, often has an extremely sandy texture that makes them fragile—highly prone to water and wind erosion. Human activities have converted or degraded many areas of this biome. In this review we discuss our state-of-the-art knowledge of the diversity and the major biological features of this regions and the cultural factors that have shaped it. Our aim is to contribute toward a better understanding of the current status of this special biome and to describe how the interaction between human activities and environment affects the region, highlighting the fragility of the Brazilian Pampa.

Neurotrophic factors comprise essential secreted proteins that have several functions in neural and non-neural tissues, mediating the development, survival and maintenance of peripheral and central nervous system. Therefore, neurotrophic factor issue has been extensively investigated into the context of neurodegenerative diseases. Alzheimer's disease and Parkinson's disease show changes in the regulation of specific neurotrophic factors and their receptors, which appear to be critical for neuronal degeneration. Indeed, neurotrophic factors prevent cell death in degenerative processes and can enhance the growth and function of affected neurons in these disorders. Based on recent reports, this review discusses the main findings related to the neurotrophic factor support - mainly brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor - in the survival, proliferation and maturation of affected neurons in Alzheimer's disease and Parkinson's disease as well as their putative application as new therapeutic approach for these diseases management.

As daily life grinds to a halt worldwide in response to the coronavirus disease 2019 (COVID-19) pandemic, professionals are adjusting to a new reality of remote working. For many researchers, the release from teaching and administrative activities means more time for independent work. In contrast, parents of young children for whom school has been cancelled are facing uniquely challenging responsibilities. Although academic fathers are not immune to the impacts of confinement, it is traditionally women who carry the heaviest load ([ 1 ][1], [ 2 ][2]). These women risk suffering yet another motherhood penalty. Instead of writing papers, they are likely to devote time to homeschooling children and doing household chores. For those who have not yet leaked from the pipeline ([ 3 ][3]) and are struggling to keep their careers on track, these months of heavier duties may increase the distance between them and their male and childless peers. Gender inequality in science is an urgent issue, and motherhood plays a major role in it ([ 4 ][4]). Recent years have witnessed the emergence of many initiatives that ignited changes toward addressing this problem [e.g., ([ 5 ][5]–[ 8 ][6])]. We cannot allow this pandemic to reverse advances and further deepen the gender gap in science. Policies and actions to mitigate the motherhood penalty can benefit all scientists. Deadlines for grant proposals, reports, and renewal requests must be postponed. Funding agencies should consider creating granting programs designed around the reality of academics with families. By instituting more flexible policies, we can make science fairer for everyone affected by the pandemic. 1. [↵][7]1. L. S. Machado et al ., “Parent in science: The impact of parenthood on the scientific career in Brazil,” Proceedings of the 2nd International Workshop on Gender Equality in Software Engineering (2019), pp. 37–40. 2. [↵][8]1. M. A. Mason et al ., Do Babies Matter?: Gender and Family in the Ivory Tower (Rutgers University Press, New Brunswick, NJ, 2013). 3. [↵][9]1. J. Huang et al ., Proc. Natl. Acad. Sci. U.S.A. 117, 4609 (2020). [OpenUrl][10][Abstract/FREE Full Text][11] 4. [↵][12]1. E. A. Cech, 2. M. Blair-Loy , Proc. Natl. Acad Sci U.S.A. 116, 4182 (2019). [OpenUrl][13][Abstract/FREE Full Text][14] 5. [↵][15]1. M. Mills 1. V. A. Jean et al ., in Gender and the Work-Family Experience, M. Mills, Ed. (Springer, 2015), pp. 291–311. 6. Parent in Science ([www.parentinscience.com][16]). 7. Mothers in Science ([www.mothersinscience.com][17]). 8. [↵][18]Mama Is an Academic ( ). [1]: #ref-1 [2]: #ref-2 [3]: #ref-3 [4]: #ref-4 [5]: #ref-5 [6]: #ref-8 [7]: #xref-ref-1-1 View reference 1 in text [8]: #xref-ref-2-1 View reference 2 in text [9]: #xref-ref-3-1 View reference 3 in text [10]: {openurl}?query=rft.jtitle%253DProc.%2BNatl.%2BAcad.%2BSci.%2BU.S.A.%26rft_id%253Dinfo%253Adoi%252F10.1073%252Fpnas.1914221117%26rft_id%253Dinfo%253Apmid%252F32071248%26rft.genre%253Darticle%26rft_val_fmt%253Dinfo%253Aofi%252Ffmt%253Akev%253Amtx%253Ajournal%26ctx_ver%253DZ39.88-2004%26url_ver%253DZ39.88-2004%26url_ctx_fmt%253Dinfo%253Aofi%252Ffmt%253Akev%253Amtx%253Actx [11]: /lookup/ijlink/YTozOntzOjQ6InBhdGgiO3M6MTQ6Ii9sb29rdXAvaWpsaW5rIjtzOjU6InF1ZXJ5IjthOjQ6e3M6ODoibGlua1R5cGUiO3M6NDoiQUJTVCI7czoxMToiam91cm5hbENvZGUiO3M6NDoicG5hcyI7czo1OiJyZXNpZCI7czoxMDoiMTE3LzkvNDYwOSI7czo0OiJhdG9tIjtzOjI0OiIvc2NpLzM2OC82NDkyLzcyNC4xLmF0b20iO31zOjg6ImZyYWdtZW50IjtzOjA6IiI7fQ== [12]: #xref-ref-4-1 View reference 4 in text [13]: {openurl}?query=rft.jtitle%253DProc.%2BNatl.%2BAcad%2BSci%2BU.S.A.%26rft_id%253Dinfo%253Adoi%252F10.1073%252Fpnas.1810862116%26rft_id%253Dinfo%253Apmid%252F30782835%26rft.genre%253Darticle%26rft_val_fmt%253Dinfo%253Aofi%252Ffmt%253Akev%253Amtx%253Ajournal%26ctx_ver%253DZ39.88-2004%26url_ver%253DZ39.88-2004%26url_ctx_fmt%253Dinfo%253Aofi%252Ffmt%253Akev%253Amtx%253Actx [14]: /lookup/ijlink/YTozOntzOjQ6InBhdGgiO3M6MTQ6Ii9sb29rdXAvaWpsaW5rIjtzOjU6InF1ZXJ5IjthOjQ6e3M6ODoibGlua1R5cGUiO3M6NDoiQUJTVCI7czoxMToiam91cm5hbENvZGUiO3M6NDoicG5hcyI7czo1OiJyZXNpZCI7czoxMToiMTE2LzEwLzQxODIiO3M6NDoiYXRvbSI7czoyNDoiL3NjaS8zNjgvNjQ5Mi83MjQuMS5hdG9tIjt9czo4OiJmcmFnbWVudCI7czowOiIiO30= [15]: #xref-ref-5-1 View reference 5 in text [16]: http://www.parentinscience.com [17]: http://www.mothersinscience.com [18]: #xref-ref-8-1 View reference 8 in text

Susceptibility to many human autoimmune diseases is under strong genetic control by class II human leukocyte antigen (HLA) allele combinations. These genes remain by far the greatest risk factors in the development of type 1 diabetes and celiac disease. Despite this, little is known about HLA influences on the composition of the human gut microbiome, a potential source of environmental influence on disease. Here, using a general population cohort from the All Babies in Southeast Sweden study, we report that genetic risk for developing type 1 diabetes autoimmunity is associated with distinct changes in the gut microbiome. Both the core microbiome and beta diversity differ with HLA risk group and genotype. In addition, protective HLA haplotypes are associated with bacterial genera Intestinibacter and Romboutsia. Thus, general population cohorts are valuable in identifying potential environmental triggers or protective factors for autoimmune diseases that may otherwise be masked by strong genetic control.

Transformerless inverters are extensively employed in grid-connected photovoltaic (PV) generation systems due to its advantages of achieving low cost and high efficiency. However, the common-mode voltage issues have been motivated the proposition of new topologies, control, and modulation schemes. In common ground PV inverters, the grid neutral line is directly connected to the negative pole of the dc bus. Therefore, the parasitic capacitances are bypassed and the leakage current can be eliminated. In this paper, a five-level common ground transformerless inverter with reduced output harmonic content for PV systems is proposed. In addition, the proposed inverter can process reactive power and it presents a maximum dc-voltage utilization in opposition to half-bridge-based topologies. The operation modes of the proposed inverter, a simple modulation strategy, as well as the design guidelines are analyzed in detail. Finally, experimental results demonstrate the feasibility and good performance of the proposed inverter.

This paper presents a discrete-time adaptive current controller for grid-connected pulse width modulation voltage source converters with LCL filter. The main attribute of the proposed current controller is that, in steady state, the damping of the LCL resonance does not depend on the grid characteristic since the adaptive feedback gains ensure a predefined behavior for the closed-loop current control. An overview of model reference adaptive state feedback theory is presented aiming to give the reader the required background for the adaptive current control design. The digital implementation delay is included in the model, and the stability concerning the variation of the grid parameters is analyzed in detail. Furthermore, current distortions due to the grid background voltage are rejected without using the conventional stationary resonant controllers or the synchronous proportional-plus-integral controllers. Simulation and experimental results are presented to validate the analysis and to demonstrate the good performance of the proposed controller for grid-connected converters subjected to large grid impedance variation and grid voltage disturbances.

We update the SMC, Bridge, and LMC catalogues of extended objects that were constructed by members of our group from 1995 to 2000. In addition to the rich subsequent literature for the previous classes, we now also include HI shells and supershells. A total of 9305 objects were cross-identified, while our previous catalogues amounted to 7900 entries, an increase of $\approx12%$. We present the results in subcatalogues containing 1445 emission nebulae, 3740 star clusters, 3326 associations, and 794 HI shells and supershells. Angular and apparent size distributions of the extended objects are analysed. We conclude that the objects, in general, appear to respond to tidal effects arising from the LMC, SMC, and Bridge. Number-density profiles extracted along directions parallel and perpendicular to the LMC bar, can be described by two exponential-disks. A single exponential-disk fits the equivalent SMC profiles. Interestingly, when angular-averaged number-densities of most of the extended objects are considered, the profiles of both Clouds do not follow an exponential-disk. Rather, they are best described by a tidally-truncated, core/halo profile, despite the fact that the Clouds are clearly disturbed disks. On the other hand, the older star clusters taken isolately, distribute as an exponential disk. The present catalogue is an important tool for the unambiguous identification of previous objects in current CCD surveys and to establish new findings.

C-Phycocyanin is a natural blue dye used in food and pharmaceutical industry. In the present study, a simple and efficient method to extract C-phycocyanin from Spirulina platensis wet biomass is reported. The extractions were carried out using six different methods, including chemical (organic and inorganic acid treatment), physical (freezing and thawing, sonication, homogenization) and enzymatic (lysozyme treatment) methods. The extraction using ultrasonic bath in the presence of glass pearls in the biomass proved to be the most efficient method, 56% higher than using freezing and thawing (the method most frequently used), and presented a extraction yield of 43.75 mg.g-1 and a C-phycocyanin concentration of 0.21 mg.mL-1.

Abstract The growing demand for minerals has pushed mining activities into new areas increasingly affecting biodiversity-rich natural biomes. Mapping the land use of the global mining sector is, therefore, a prerequisite for quantifying, understanding and mitigating adverse impacts caused by mineral extraction. This paper updates our previous work mapping mining sites worldwide. Using visual interpretation of Sentinel-2 images for 2019, we inspected more than 34,000 mining locations across the globe. The result is a global-scale dataset containing 44,929 polygon features covering 101,583 km 2 of large-scale as well as artisanal and small-scale mining. The increase in coverage is substantial compared to the first version of the dataset, which included 21,060 polygons extending over 57,277 km 2 . The polygons cover open cuts, tailings dams, waste rock dumps, water ponds, processing plants, and other ground features related to the mining activities. The dataset is available for download from https://doi.org/10.1594/PANGAEA.942325 and visualisation at www.fineprint.global/viewer .

BACKGROUND: Heart failure (HF) is a complex syndrome and is recognized as the ultimate pathway of cardiovascular disease (CVD). Studies using nutritional strategies based on dietary patterns have proved to be effective for the prevention and treatment of CVD. Although there are studies that support the protective effect of these diets, their effects on the prevention of HF are not clear yet. METHODS: We searched the Medline, Embase, and Cochrane databases for studies that examined dietary patterns, such as dietary approaches to stop hypertension (DASH diet), paleolithic, vegetarian, low-carb and low-fat diets and prevention of HF. No limitations were used during the search in the databases. RESULTS: A total of 1119 studies were identified, 14 met the inclusion criteria. Studies regarding the Mediterranean, DASH, vegetarian, and Paleolithic diets were found. The Mediterranean and DASH diets showed a protective effect on the incidence of HF and/or worsening of cardiac function parameters, with a significant difference in relation to patients who did not adhere to these dietary patterns. CONCLUSIONS: It is observed that the adoption of Mediterranean or DASH-type dietary patterns may contribute to the prevention of HF, but these results need to be analyzed with caution due to the low quality of evidence.

Indoor environments contribute significantly to total human exposure to air pollutants, as people spend most of their time indoors. Household air pollution (HAP) resulting from cooking with polluting ("dirty") fuels, which include coal, kerosene, and biomass (wood, charcoal, crop residues, and animal manure) is a global environmental health problem. Indoor pollutants are gases, particulates, toxins, and microorganisms among others, that can have an impact especially on the health of children and adults through a combination of different mechanisms on oxidative stress and gene activation, epigenetic, cellular, and immunological systems. Air pollution is a major risk factor and contributor to morbidity and mortality from major chronic diseases. Children are significantly affected by the impact of the environment due to biological immaturity, prenatal and postnatal lung development. Poor air quality has been related to an increased prevalence of clinical manifestations of allergic asthma and rhinitis. Health professionals should increase their role in managing the exposure of children and adults to air pollution with better methods of care, prevention, and collective action. Interventions to reduce household pollutants may promote health and can be achieved with education, community, and health professional involvement.