Altai State University

We present new parameterizations of energy spectra of secondary particles, $\ensuremath{\pi}$ mesons, gamma rays, electrons, and neutrinos produced in inelastic proton-proton collisions. The simple analytical approximations based on simulations of proton-proton interactions using the public available SIBYLL code provide very good accuracy for energy distributions of secondary products in the energy range above 100 GeV. Generally, the recommended analytical formulas deviate from the simulated distributions within a few percent over a large range of $x={E}_{i}/{E}_{p}$---the fraction of energy of the incident proton transferred to the secondaries. Finally, we describe an approximate procedure of continuation of calculations towards low energies, down to the threshold of $\ensuremath{\pi}$-meson production.

By sequencing 523 ancient humans, we show that the primary source of ancestry in modern South Asians is a prehistoric genetic gradient between people related to early hunter-gatherers of Iran and Southeast Asia. After the Indus Valley Civilization's decline, its people mixed with individuals in the southeast to form one of the two main ancestral populations of South Asia, whose direct descendants live in southern India. Simultaneously, they mixed with descendants of Steppe pastoralists who, starting around 4000 years ago, spread via Central Asia to form the other main ancestral population. The Steppe ancestry in South Asia has the same profile as that in Bronze Age Eastern Europe, tracking a movement of people that affected both regions and that likely spread the distinctive features shared between Indo-Iranian and Balto-Slavic languages.

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Although a rich record of Pleistocene human-associated archaeological assemblages exists, the scarcity of hominin fossils often impedes the understanding of which hominins occupied a site. Using targeted enrichment of mitochondrial DNA, we show that cave sediments represent a rich source of ancient mammalian DNA that often includes traces of hominin DNA, even at sites and in layers where no hominin remains have been discovered. By automation-assisted screening of numerous sediment samples, we detected Neandertal DNA in eight archaeological layers from four caves in Eurasia. In Denisova Cave, we retrieved Denisovan DNA in a Middle Pleistocene layer near the bottom of the stratigraphy. Our work opens the possibility of detecting the presence of hominin groups at sites and in areas where no skeletal remains are found.

Abstract Domestication of horses fundamentally transformed long-range mobility and warfare 1 . However, modern domesticated breeds do not descend from the earliest domestic horse lineage associated with archaeological evidence of bridling, milking and corralling 2–4 at Botai, Central Asia around 3500 bc 3 . Other longstanding candidate regions for horse domestication, such as Iberia 5 and Anatolia 6 , have also recently been challenged. Thus, the genetic, geographic and temporal origins of modern domestic horses have remained unknown. Here we pinpoint the Western Eurasian steppes, especially the lower Volga-Don region, as the homeland of modern domestic horses. Furthermore, we map the population changes accompanying domestication from 273 ancient horse genomes. This reveals that modern domestic horses ultimately replaced almost all other local populations as they expanded rapidly across Eurasia from about 2000 bc , synchronously with equestrian material culture, including Sintashta spoke-wheeled chariots. We find that equestrianism involved strong selection for critical locomotor and behavioural adaptations at the GSDMC and ZFPM1 genes. Our results reject the commonly held association 7 between horseback riding and the massive expansion of Yamnaya steppe pastoralists into Europe around 3000 bc 8,9 driving the spread of Indo-European languages 10 . This contrasts with the scenario in Asia where Indo-Iranian languages, chariots and horses spread together, following the early second millennium bc Sintashta culture 11,12 .

Abstract Angiosperms are the cornerstone of most terrestrial ecosystems and human livelihoods 1,2 . A robust understanding of angiosperm evolution is required to explain their rise to ecological dominance. So far, the angiosperm tree of life has been determined primarily by means of analyses of the plastid genome 3,4 . Many studies have drawn on this foundational work, such as classification and first insights into angiosperm diversification since their Mesozoic origins 5–7 . However, the limited and biased sampling of both taxa and genomes undermines confidence in the tree and its implications. Here, we build the tree of life for almost 8,000 (about 60%) angiosperm genera using a standardized set of 353 nuclear genes 8 . This 15-fold increase in genus-level sampling relative to comparable nuclear studies 9 provides a critical test of earlier results and brings notable change to key groups, especially in rosids, while substantiating many previously predicted relationships. Scaling this tree to time using 200 fossils, we discovered that early angiosperm evolution was characterized by high gene tree conflict and explosive diversification, giving rise to more than 80% of extant angiosperm orders. Steady diversification ensued through the remaining Mesozoic Era until rates resurged in the Cenozoic Era, concurrent with decreasing global temperatures and tightly linked with gene tree conflict. Taken together, our extensive sampling combined with advanced phylogenomic methods shows the deep history and full complexity in the evolution of a megadiverse clade.

In order to explore the diversity and selective signatures of duplication and deletion human copy-number variants (CNVs), we sequenced 236 individuals from 125 distinct human populations. We observed that duplications exhibit fundamentally different population genetic and selective signatures than deletions and are more likely to be stratified between human populations. Through reconstruction of the ancestral human genome, we identify megabases of DNA lost in different human lineages and pinpoint large duplications that introgressed from the extinct Denisova lineage now found at high frequency exclusively in Oceanic populations. We find that the proportion of CNV base pairs to single-nucleotide-variant base pairs is greater among non-Africans than it is among African populations, but we conclude that this difference is likely due to unique aspects of non-African population history as opposed to differences in CNV load.

The Crab supernova remnant has been observed regularly with the stereoscopic system of 5 imaging air Cherenkov telescopes that was part of the High Energy Gamma Ray Astronomy (HEGRA) experiment. In total, close to 400 hours of useful data have been collected from 1997 until 2002. The spectrum extends up to energies of 80 TeV and is well matched by model calculations in the framework of inverse Compton scattering of various seed photons in the nebula including for the first time a recently detected compact emission region at mm-wavelengths. The observed indications for a gradual steepening of the energy spectrum in data is expected in the inverse Compton emission model.The average magnetic field in the emitting volume is determined to be $(161.6\pm0.8mathrm{stat}\pm18_\mathrm{sys}) \mu$G. The presence of protons in the nebula is not required to explain the observed flux and upper limits on the injected power of protons are calculated being as low as 20 % of the total spin down luminosity for bulk Lorentz factors of the wind in the range of $10^4-10^6$.The position and size of the emission region have been studied over a wide range of energies. The position is shifted by 13\arcsec to the west of the pulsar with a systematic uncertainty of 25\arcsec. No significant shift in the position with energy is observed. The size of the emission region is constrained to be less than 2\arcmin at energies between 1 and 10 TeV. Above 30 TeV the size is constrained to be less than 3\arcmin.No indications for pulsed emission has been found and upper limits in differential bins of energy have been calculated reaching typically 1-3 % of the unpulsed component.

232 hours of data were accumulated from 1997 to 1999, using the HEGRA Stereoscopic Cherenkov Telescope System to observe the supernova remnant Cassiopeia A. TeV γ-ray emission was detected at the level, and a flux of above 1 TeV was derived. The spectral distribution is consistent with a power law with a differential spectral index of between 1 and 10 TeV. As this is the first report of the detection of a TeV γ-ray source on the "centi-Crab"scale, we present the analysis in some detail. Implications for the acceleration of cosmic rays depend on the details of the source modeling. We discuss some important aspects in this paper.

Denisovans, a sister group of Neandertals, have been described on the basis of a nuclear genome sequence from a finger phalanx (Denisova 3) found in Denisova Cave in the Altai Mountains. The only other Denisovan specimen described to date is a molar (Denisova 4) found at the same site. This tooth carries a mtDNA sequence similar to that of Denisova 3. Here we present nuclear DNA sequences from Denisova 4 and a morphological description, as well as mitochondrial and nuclear DNA sequence data, from another molar (Denisova 8) found in Denisova Cave in 2010. This new molar is similar to Denisova 4 in being very large and lacking traits typical of Neandertals and modern humans. Nuclear DNA sequences from the two molars form a clade with Denisova 3. The mtDNA of Denisova 8 is more diverged and has accumulated fewer substitutions than the mtDNAs of the other two specimens, suggesting Denisovans were present in the region over an extended period. The nuclear DNA sequence diversity among the three Denisovans is comparable to that among six Neandertals, but lower than that among present-day humans.

Deep observation (~113 hrs) of the Cygnus region at TeV energies using the HEGRA stereoscopic system of air Čerenkov telescopes has serendipitously revealed a signal positionally inside the core of the OB association Cygnus OB2, at the edge of the 95% error circle of the EGRET source 3EG J2033+4118, and ~ north of Cyg X-3. The source centre of gravity is RA : , Dec : . The source is steady, has a post-trial significance of +4.6σ, indication for extension with radius at the ~ level, and has a differential power-law flux with hard photon index of . The integral flux above 1 TeV amounts ~3% that of the Crab. No counterpart for the TeV source at other wavelengths is presently identified, and its extension would disfavour an exclusive pulsar or AGN origin. If associated with Cygnus OB2, this dense concentration of young, massive stars provides an environment conducive to particle acceleration and likely subsequent interaction with a nearby gas cloud. Alternatively, one could envisage γ-ray production via a jet-driven termination shock.

For the first time an excess of photons above an energy threshold of 730 GeV from the giant radio galaxy M 87 has been measured at a significance level above 4 . The data have been taken during the years 1998 and 1999 with the HEGRA stereoscopic system of 5 imaging atmospheric Cherenkov telescopes. The excess of 107.4 26.8 events above 730 GeV corresponds to an integral flux of 3.3% of the Crab flux or N (E > 730 GeV) = (0.96 0.23) 10 -12 phot cm -2 s -1 . M 87 is located at the center of the Virgo cluster of galaxies at a relatively small redshift of z = 0.00436 and is a promising candidate among the class of giant radio galaxies for the emission of TeV -radiation. The detection of TeV -rays from M 87 -if confirmed -would establish a new class of extragalactic source in this energy regime since all other AGN detected to date at TeV energies are BL Lac type objects.

Fungi are highly important biotic components of terrestrial ecosystems, but we still have a very limited understanding about their diversity and distribution. This data article releases a global soil fungal dataset of the Global Soil Mycobiome consortium (GSMc) to boost further research in fungal diversity, biogeography and macroecology. The dataset comprises 722,682 fungal operational taxonomic units (OTUs) derived from PacBio sequencing of full-length ITS and 18S-V9 variable regions from 3200 plots in 108 countries on all continents. The plots are supplied with geographical and edaphic metadata. The OTUs are taxonomically and functionally assigned to guilds and other functional groups. The entire dataset has been corrected by excluding chimeras, index-switch artefacts and potential contamination. The dataset is more inclusive in terms of geographical breadth and phylogenetic diversity of fungi than previously published data. The GSMc dataset is available over the PlutoF repository.

A technological breakthrough in simultaneously tracking the visual behavior of two people with an eye tracker (DUET) allows you to explore how a child perceives the world and how an adult (teacher) infl uences it. It became possible to more objectively trace the transformation of a child’s perceptual processes under the infl uence of learning (Monroy, Chen, Houston, Yu, ). An analysis of the gaze movements allows us to model the learning process as the emergence and dynamic transformation of an intersubjective connection between the perception-action systems of a child and an adult (Shvarts, ). Synchronous registration of the dyad’s eye movements was carried out by two portable trackers in the form of Pupil Headset goggles. A comparative study was carried out on a sample of preschoolers - years old: typically developing children and children with hearing impairment after cochlear implantation (sensoneural hearing loss, ICD- class H). An analysis of the ways in which the gaze of the adult-child dyad moves makes it possible to model the learning process as the emergence and dynamic transformation of an intersubjective connection between the perception-action systems of a child and an adult. Comparison of gaze patterns showed that contrasting groups of children use different perceptual strategies in the learning process: the specifi city of eye movements of contrasting groups is manifested in the perceptual actions themselves and the pattern of eye movements relative to fi xations in relevant areas corresponding to the task. It was found that the oculomotor activity of an adult changes in the process of interaction with children of contrasting groups and is organized taking into account the specifi c features of the child’s perceptual activity. Differences are manifested in the degree of synchrony of fi xations and the similarity of the trajectories of gaze movements of an adult and a child. An important result of using the DUET technology on contrast samples is the fact that the process of teaching/ learning caused a mutual transformation of the perceptual strategies of both the adult and the child. The perceptual connection of the dyad is clearly manifested in tracking, identifying and controlling perceptual actions as a previous phase, the emergence of compatibility.

Fungi are highly diverse organisms, which provide multiple ecosystem services. However, compared with charismatic animals and plants, the distribution patterns and conservation needs of fungi have been little explored. Here, we examined endemicity patterns, global change vulnerability and conservation priority areas for functional groups of soil fungi based on six global surveys using a high-resolution, long-read metabarcoding approach. We found that the endemicity of all fungi and most functional groups peaks in tropical habitats, including Amazonia, Yucatan, West-Central Africa, Sri Lanka, and New Caledonia, with a negligible island effect compared with plants and animals. We also found that fungi are predominantly vulnerable to drought, heat and land-cover change, particularly in dry tropical regions with high human population density. Fungal conservation areas of highest priority include herbaceous wetlands, tropical forests, and woodlands. We stress that more attention should be focused on the conservation of fungi, especially root symbiotic arbuscular mycorrhizal and ectomycorrhizal fungi in tropical regions as well as unicellular early-diverging groups and macrofungi in general. Given the low overlap between the endemicity of fungi and macroorganisms, but high conservation needs in both groups, detailed analyses on distribution and conservation requirements are warranted for other microorganisms and soil organisms.

Angiosperms have become the dominant terrestrial plant group by diversifying for ~145 million years into a broad range of environments. During the course of evolution, numerous morphological innovations arose, often preceded by whole genome duplications (WGD). The mustard family (Brassicaceae), a successful angiosperm clade with ~4000 species, has been diversifying into many evolutionary lineages for more than 30 million years. Here we develop a species inventory, analyze morphological variation, and present a maternal, plastome-based genus-level phylogeny. We show that increased morphological disparity, despite an apparent absence of clade-specific morphological innovations, is found in tribes with WGDs or diversification rate shifts. Both are important processes in Brassicaceae, resulting in an overall high net diversification rate. Character states show frequent and independent gain and loss, and form varying combinations. Therefore, Brassicaceae pave the way to concepts of phylogenetic genome-wide association studies to analyze the evolution of morphological form and function.

The nearby BL Lacertae (BL Lac) object Markarian 421 (Mkn 421) at a red shift was observed to undergo strong TeV γ-ray outbursts in the observational periods from December 1999 until May 2001. The time averaged flux level in the 1999/2000 season was 10-11 ph cm-2 s-1, whereas in the 2000/2001 season the average integral flux increased to 10-11 ph cm-2 s-1. Both energy spectra are curved and well fit by a power law with an exponential cut-off energy at TeV. The respective energy spectra averaged over each of the two time periods indicate a spectral hardening for the 2000/2001 spectrum. The photon index changes from for 1999/2000 to in 2000/2001. The energy spectra derived for different average flux levels ranging from 0.5 to 10 10-11 ph cm-2 s-1 follow a clear correlation of photon index and flux level. Generally, the energy spectra are harder for high flux levels. From January to April 2001 Mkn 421 showed rapid variability (doubling time as short as 20 min), accompanied with a spectral hardening with increasing flux level within individual nights. For two successive nights (MJD 51989-51991, March 21-23, 2001), this correlation of spectral hardness and change in flux has been observed within a few hours. The cut-off energy for the Mkn 421 TeV spectrum remains within the errors constant for the different flux levels and differs by TeV from the value determined for Mkn 501. This indicates that the observed exponential cut-off in the energy spectrum of Mkn 421 is not solely caused by absorption of multi-TeV photons by pair-production processes with photons of the extragalactic near/mid infrared background radiation.

The effect of nanoparticle size and structure on XRD and SAXS patterns was investigated using modeling with the Debye scattering equation for a series of nanoparticles (NP) with the positions of atoms kept according to the bulk lattice and after structure relaxation. The purpose of research was to determine if the changes in XRD peak positions for NP are entirely due to the well-known effects of lattice parameter change or if additional effects can arise from the size itself. It was found that for very small NPs with sizes below 5 nm, the size itself influences the XRD patterns. This effect can be caused by interference fringes and is not taken into account when considering XRD patterns in standard software. The research demonstrates that new methods for XRD pattern treatment of very small nanoparticles should be developed.

The unidentified TeV source in Cygnus is now confirmed by follow-up observations from 2002 with the HEGRA stereoscopic system of Cherenkov Telescopes. Using all data (1999 to 2002) we confirm this new source as steady in flux over the four years of data taking, extended with radius 6.2 (1.2 stat 0.9 sys ) and exhibiting a hard spectrum with photon index -1.9. It is located in the direction of the dense OB stellar association, Cygnus OB2. Its integral flux above energies E > 1 TeV amounts to 5% of the Crab assuming a Gaussian profile for the intrinsic source morphology. There is no obvious counterpart at radio, optical nor X-ray energies, leaving TeV J2032+4130 presently unidentified. Observational parameters of this source are updated here and some astrophysical discussion is provided. Also included are upper limits for a number of other interesting sources in the FoV, including the famous microquasar Cygnus X-3.

The mustard family (Brassicaceae) is a scientifically and economically important family, containing the model plant Arabidopsis thaliana and numerous crop species that feed billions worldwide. Despite its relevance, most phylogenetic trees of the family are incompletely sampled and often contain poorly supported branches. Here, we present the most complete Brassicaceae genus-level family phylogenies to date (Brassicaceae Tree of Life or BrassiToL) based on nuclear (1,081 genes, 319 of the 349 genera; 57 of the 58 tribes) and plastome (60 genes, 265 genera; all tribes) data. We found cytonuclear discordance between the two, which is likely a result of rampant hybridization among closely and more distantly related lineages. To evaluate the impact of such hybridization on the nuclear phylogeny reconstruction, we performed five different gene sampling routines, which increasingly removed putatively paralog genes. Our cleaned subset of 297 genes revealed high support for the tribes, whereas support for the main lineages (supertribes) was moderate. Calibration based on the 20 most clock-like nuclear genes suggests a late Eocene to late Oligocene origin of the family. Finally, our results strongly support a recently published new family classification, dividing the family into two subfamilies (one with five supertribes), together representing 58 tribes. This includes five recently described or re-established tribes, including Arabidopsideae, a monogeneric tribe accommodating Arabidopsis without any close relatives. With a worldwide community of thousands of researchers working on Brassicaceae and its diverse members, our new genus-level family phylogeny will be an indispensable tool for studies on biodiversity and plant biology.