Délégation Ile-de-France Sud

Rickettsia-like maternally inherited bacteria have been shown to be involved in a variety of alterations of arthropod sexuality, such as female-biased sex ratios, parthenogenesis, and sterility of crosses either between infected males and uninfected females or between infected individuals (cytoplasmic incompatibility). We have characterized several of these microorganisms through partial sequences of the small (16S) and large (23S) subunit ribosomal DNA. All the symbionts identified, which include several cytoplasmic incompatibility microorganisms, several endosymbionts of terrestrial isopods, and symbionts of two thelytokous Trichogramma wasp species, belong to a monophyletic group of related symbionts, some of which have previously been detected in several insects exhibiting cytoplasmic incompatibility. Three molecular lineages can be identified on the basis of 16S as well as 23S sequences. Although they are only known as endocellular symbionts, Wolbachia spread by horizontal transfer across host lineages as evidenced by their diversification which occurred long after that of their hosts, and by the non-congruence of the phylogenetic relationships of symbionts and their hosts. Indeed, symbionts of two different lineages have been found in the same host species, whereas closely related endosymbionts are found in distinct insect orders. Isopod endosymbionts form a separate lineage, and they can determine feminization as well as cytoplasmic incompatibility. The ability to determine cytoplasmic incompatibility, found in all lineages, is probably ancestral to this group.

In the past decade, the fast and transient coupling and uncoupling of functionally related brain regions into networks has received much attention in cognitive neuroscience. Empirical tools to study network coupling include functional magnetic resonance imaging (fMRI)-based functional and/or effective connectivity, and electroencephalography (EEG)/magnetoencephalography-based measures of neuronal synchronization. Here we use simultaneously recorded EEG and fMRI to assess whether fMRI-based connectivity and frequency-specific EEG power are related. Using data collected during resting state, we studied whether posterior EEG alpha power fluctuations are correlated with connectivity within the visual network and between the visual cortex and the rest of the brain. The results show that when alpha power increases, BOLD connectivity between the primary visual cortex and occipital brain regions decreases and that the negative relation of the visual cortex with the anterior/medial thalamus decreases and the ventral-medial prefrontal cortex is reduced in strength. These effects were specific for the alpha band, and not observed in other frequency bands. The decreased connectivity within the visual system may indicate an enhanced functional inhibition during a higher alpha activity. This higher inhibition level also attenuates long-range intrinsic functional antagonism between the visual cortex and the other thalamic and cortical regions. Together, these results illustrate that power fluctuations in posterior alpha oscillations result in local and long-range neural connectivity changes.

A scheelite crystal with residual concentration of erbium is cooled down to 10 mK, yielding a record spin coherence time of 23 ms.

Interferons (IFNs) are a family of secreted proteins with antiviral, antiproliferative, and immunomodulatory activities. The different biologic actions of IFN are believed to be mediated by the products of specifically IFN-stimulated genes (ISG) in the target cells. The IFN response is the first line of defense against viral infections. Viruses, which require the cellular machinery for their replication, have evolved different ways to counteract the action of IFN by inhibiting IFN production or Jak-Stat signaling or by altering ISG products. This review focuses on the role of viral proteins from the RNA virus family, particularly rabies P protein. P protein mediates inhibition of the IFN system by different pathways: it inhibits IFN production by impairing IFN regulatory factor-3 (IRF-3) phosphorylation and IFN signaling by blocking nuclear transport of Stat1 and alters promyelocytic leukemia (PML) nuclear bodies by retaining PML in the cytoplasm.

Due to the relative transparency of its embryos and larvae, the zebrafish is an ideal model organism for bioimaging approaches in vertebrates. Novel microscope technologies allow the imaging of developmental processes in unprecedented detail, and they enable the use of complex image-based read-outs for high-throughput/high-content screening. Such applications can easily generate Terabytes of image data, the handling and analysis of which becomes a major bottleneck in extracting the targeted information. Here, we describe the current state of the art in computational image analysis in the zebrafish system. We discuss the challenges encountered when handling high-content image data, especially with regard to data quality, annotation, and storage. We survey methods for preprocessing image data for further analysis, and describe selected examples of automated image analysis, including the tracking of cells during embryogenesis, heartbeat detection, identification of dead embryos, recognition of tissues and anatomical landmarks, and quantification of behavioral patterns of adult fish. We review recent examples for applications using such methods, such as the comprehensive analysis of cell lineages during early development, the generation of a three-dimensional brain atlas of zebrafish larvae, and high-throughput drug screens based on movement patterns. Finally, we identify future challenges for the zebrafish image analysis community, notably those concerning the compatibility of algorithms and data formats for the assembly of modular analysis pipelines.

In order to investigate the effects of a partial cholinergic deafferentation on the functional activity of the cortex, the cerebral metabolic rate of glucose (CMRGlu) was measured with positron emission tomography and 18F-2-fluoro-2-deoxy-D-glucose in 5 baboons (Papio anubis) both before and serially following stereotaxic electrocoagulation of the left nucleus basalis of Meynert (NbM). Four days postlesion, significant metabolic depression was present in the entire ipsilateral cerebral cortex, most marked in the frontotemporal region, and which slowly recovered close to normal within 6-13 weeks. Postmortem studies showed that the lesions were located largely in the NbM, and that a significant decrease in choline-acetyltransferase (ChAT) activity was present in the ipsilateral frontal, temporal and parietal cortices. The animal with the most limited histological lesion showed the least decrease in both ChAT activity and CMRGlu. There was a highly significant linear correlation between the regional cortical decreases in CMRGlu (early postlesion data) and in ChAT activity. These results indicate that cholinergic deafferentation induces a proportional metabolic depression in the cortex. However, compensatory mechanisms operate to restore the cortical metabolic activity gradually despite sustained cholinergic denervation, pointing to pre- and/or postsynaptic adaptation (plasticity). Moreover, unilateral NbM lesions also induced a significant reduction in contralateral CMRGlu, which also demonstrated recovery. Several mechanisms are discussed to explain this contralateral effect, but the most likely implicates a transcallosal depression of function as a result of ipsilateral effects of the NbM lesion. These metabolic effects of cholinergic deafferentation in the primate provide new insight into the mechanisms of cortical dysfunction, and the recovery thereof, following subcortical lesions. In addition, our findings have some relevance to the cortical consequences of cholinergic deafferentation observed in dementia of Alzheimer type.

This paper explores some of the difficulties in accounting for the value of knowledge intermediation, focusing on two types of knowledge inter-mediators: science shops and knowledge brokers. We analyse the way in which actors themselves ‘situate’, and thus value, their work and practices. Drawing upon theorisations by Antoine Hennion and Bruno Latour, who have distinguished between intermediaries and mediation, we show that actors mobilise two non-mutually exclusive, coexisting repertoires: a ‘two-world’ repertoire and a repertoire of ‘exploration’. In the two-world repertoire, knowledge inter-mediators are seen as actors placed between two (pre-existing) worlds: as a result, their task becomes connecting these two worlds. The repertoire of exploration refers to the fact that inter-mediators evolve in uncertain worlds, their role consisting of exploring and performing new worlds including their own place and role within these. We argue that the tensions between these two repertoires are a potential resource for theory and practice.

OBJECTIVES: Medication errors constitute a major problem in all hospitals. Between 20% and 46% of prescriptions requiring dosage adjustments based on renal function are inappropriate. This study aimed to determine whether implementing alerts at the time of ordering medication integrated into the computerized physician order entry decreases the proportion of inappropriate prescriptions based on the renal function of inpatients. DESIGN: Six alternating 2-month control and intervention periods were conducted between August 2006 and August 2007 in two medical departments of a teaching hospital in France. A total of 603 patients and 38 physicians were included. During the intervention periods, alerts were triggered if a patient with renal impairment was prescribed one of the 24 targeted drugs that required adjustment according to estimated glomerular filtration rate (eGFR). MEASUREMENTS: The main outcome measure was the proportion of inappropriate first prescriptions, according to recommendation. RESULTS: A total of 1,122 alerts were triggered. The rate of inappropriate first prescriptions did not differ significantly between intervention and control periods (19.9% vs. 21.3%; p=0.63). The effect of intervention differed significantly between residents and senior physicians (p=0.03). Residents tended to make fewer errors in intervention versus control periods (Odds ratio 0.69; 95% confidence interval 0.41 to 1.15), whereas senior physicians tended to make more inappropriate prescriptions in intervention periods (odds ratio 1.88; 95% confidence interval 0.91 to 3.89). CONCLUSION: Alert activation was not followed by a significant decrease in inappropriate prescriptions in our study. Thus, it is still necessary to evaluate the impact of these systems if newly implemented in other settings thanks to studies also designed to watch for possible unanticipated effects of decision supports and their underlying causes.

Optical rest-frame spectroscopic diagnostics are usually employed to distinguish between star formation and active galactic nucleus (AGN) powered emission. However, this method is biased against dusty sources, hampering a complete census of the AGN population across cosmic epochs. To mitigate this effect, it is crucial to observe at longer wavelengths in the rest-frame near-infrared (near-IR), which is less affected by dust attenuation and can thus provide a better description of the intrinsic properties of galaxies. AGN diagnostics in this regime have not been fully exploited so far, due to the scarcity of near-IR observations of both AGN and star-forming galaxies, especially at redshifts higher than 0.5. Using Cloudy photoionization models, we identified new AGN – star formation diagnostics based on the ratio of bright near-IR emission lines, namely [SIII] 9530 Å, [CI] 9850 Å, [PII] 1.188 μm, [FeII] 1.257 μm, and [FeII] 1.64 μm to Paschen lines (either Paγ or Paβ), providing simple, analytical classification criteria. We applied these diagnostics to a sample of 64 star-forming galaxies and AGN at 0 ≤ z ≤ 1, and 65 sources at 1 ≤ z ≤ 3 recently observed with JWST-NIRSpec in CEERS. We find that the classification inferred from the near-IR is broadly consistent with the optical one based on the BPT and the [SII]/Hα ratio. However, in the near-IR, we find ∼60% more AGN than in the optical (13 instead of eight), with five sources classified as “hidden” AGN, showing a larger AGN contribution at longer wavelengths, possibly due to the presence of optically thick dust. The diagnostics we present provide a promising tool to find and characterize AGN from z = 0 to z ≃ 3 with low- and medium-resolution near-IR spectrographs in future surveys.

The beta-thymosin/WH2 actin-binding module shows an amazing adaptation to multifunctionality. The beta-thymosins are genuine G-actin sequesterers of moderate affinity for G-actin, allowing an efficient regulation of the G-actin/F-actin ratio in cells by amplifying changes in the critical concentration for filament assembly. In contrast, the first beta-thymosin domain of the protein Ciboulot makes with G-actin a complex that supports filament growth, such as profilin-actin. We illustrate how the use of engineered chimeric proteins, actin-binding and polymerization assays, crystallographic, NMR, and SAXS structural approaches complement each other to decipher the molecular basis for the functional versatility of these intrinsically disordered domains when they form various 1:1 complexes with G-actin. Multifunctionality is expanded in tandem repeats of WH2 domains present in WASP family proteins and proteins involved in axis patterning like Cordon-Bleu and Spire. The tandem repeats generate new functions such as filament nucleation and severing, as well as barbed end binding, which add up to the G-actin sequestering activity. Novel regulation pathways in actin assembly emerge from these additional activities.

A Synechocytis 6714 mutant resistant to the phenol-type herbicide ioxynil was isolated and characterized. Sensitivity to DCMU and atrazine was tf measured in whole cells and isolated thylakoids. The mutant presents the same sensitivity to atrazine as the wild type and a slightly increased sensitivity to DCMU. A point mutation has been found at codon 266 in the psbAI coding locus (AAC to ACC) resulting in an amino acid change from asparagine to threonine in the D1 protein.

In this paper, a wideband dual polarized self-complementary connected array antenna with low radar cross section (RCS) under normal and oblique incidence is presented. First, an analytical model of the multilayer structure is proposed in order to obtain a fast and reliable predimensioning tool providing an optimized design of the infinite array. The accuracy of this model is demonstrated thanks to comparative simulations with a full wave analysis software. RCS reduction compared to a perfectly conducting flat plate of at least 10 dB has been obtained over an ultrawide bandwidth of nearly 7:1 at normal incidence and 5:1 (3.8 to 19 GHz) at 60° in both polarizations. These performances are confirmed by finite element tearing and interconnecting computations of finite arrays of different sizes. Finally, the realization of a 28 × 28 cell prototype and measurement results are detailed.

Endothelin B receptor (ETBR) is a G protein-coupled receptor able to bind equally to the three identified human endothelin peptides. It is expressed primarily on vascular endothelial cells and involved in various physiological processes including vascular tone homeostasis, enteric nervous system development, melanogenesis and angiogenesis. Furthermore, overactivation or overexpression of ETBR have been associated with the development of various diseases such as cardiovascular disorders and cancers. Therefore, ETBR appears to be relevant target for the therapy or diagnosis of highly prevalent human diseases. In this study, we report the in vitro characterization of rendomab-B1, a monoclonal antibody (mAb) obtained by genetic immunization, which selectively recognizes the native form of human ETBR (hETBR). Rendomab-B1 is the first-reported mAb that behaves as a potent antagonist of hETBR. It recognizes an original extracellular conformational epitope on the receptor, distinct from the endothelin-1 (ET-1) binding site. Rendomab-B1 not only blocks ET-1-induced calcium signaling pathway and triggers rapid receptor internalization on recombinant hETBR-expressing cells, but also exerts pharmacological activities on human vascular endothelial cells, reducing both cell viability and ET-1-induced hETBR synthesis. In addition, binding experiments using rendomab-B1 on different melanoma cell lines reveal the structural and functional heterogeneity of hETBR expressed at the surface of these cancer cells, strongly suggesting the existence of tumor-specific receptors. Collectively, our results underscore the value of rendomab-B1 for research, therapeutic and diagnostic applications dealing with hETBR.

We have developed in new-born mice a ventral tilted-horizontal slice preparation for pontine stimulation and recording of spontaneous respiratory-like rhythmic trains of glutamatergic excitatory postsynaptic potentials (EPSPs) in medullary neurons. Electrical stimulations (10-50 Hz for 100-500 ms) of the caudal pontine reticular formation triggered a burst of EPSPs, recycling of the rhythmic activity and persistent increase of the rhythmic behaviour. These results identify a ventral pontine pathway that promotes rhythm generating mechanisms in the medulla and probably derives from a population of lateral reticular neurons identified in the embryonic hindbrain and eliminated after inactivation of the early developmental gene Krox-20.

Hindbrain mechanisms generating the respiratory rhythm in chicks were analysed. In vivo, ventilation and intercostal muscle activity were recorded in chicks (1 and 2.5 weeks-old), vagotomized and treated with the NMDA receptor blocker MK-801 (dizocilpine). In vitro, synaptic transmission from vagal to second-order sensory neurones was studied in the nucleus of the solitary tract, using whole-cell recordings in slices. Vagal afferents were found to act through GABAergic synapses and control two hindbrain systems: a dizocilpine-sensitive control system and a rhythm generator. Although this organization is the same as in mammals, after vagotomy entirely different respiratory patterns emerge: (i) expiratory-inspiratory efforts triggered by the rhythm generator and (ii) periods of apnoea produced by the dizocilpine-sensitive system.

Abstract From fruits of Boehmeriaholosericea, six compounds 1‐6 were isolated by various chromatographic methods. Their structures were elucidated by extensive spectroscopic analysis, including ESI‐MS,1D NMR, 2D NMR spectra, they are identified as ruspolinone (1), benzyl β‐D‐glucoside (2), 3,4‐dimethoxyacetophenone (3), adenine (4), adenosine (5) and uridine (6). All the compounds were obtained for the first time from Boehmeria genus.

This paper presents a prediction-based control law for linear difference equations subject to a distributed state delay and a pointwise input delay. We propose to use a prediction-based control to overcome the instability potentially related to the distributed delay. We obtain an explicit formulation of the controller, depending only on the state and input history and involving integral kernels, which are the solutions to recursive Volterra equations. In view of future delay-sensitivity analysis, we develop an alternative approach to prove closed-loop stability, recasting the input delay as a transport Partial Differential Equation. In an analog manner to the stability analysis methodology developed for linear Delay Differential Equations, we propose a backstepping transformation to map the closed-loop system to a distributed-delay free target system. Simulation results underline the efficiency of the proposed control design.

Plasma concentrations of LH, FSH, prolactin and testosterone, pituitary concentrations of gonadotrophins and prolactin, and hypothalamic LH-RH were measured in normal, sexually mature male rats at regular intervals from 1 to 30 days after castration or cryptorchidism. Bilateral castration resulted in a marked decrease in testosterone levels 24 h after surgery. On the contrary, plasma testosterone was increased (at days 4 and 8) or unaffected by cryptorchidism when compared to intact controls. From day 1, castrated rats showed a rapid, marked increase in plasma LH and FSH (7 and 5 times higher at day 30 for LH and FSH, respectively) and a delayed, progressive increase in pituitary gonadotrophins (significant at day 8 and day 30 for LH and FSH, respectively). The cryptorchid animals showed similar but slower and less marked changes in plasma and pituitary FSH and LH levels. Unlike plasma prolactin levels, which were lowered at day 8 or days 4, 8 and 30, respectively, after castration or cryptorchidism, no change was observed in pituitary prolactin content. After castration, hypothalamic LH-RH content was significantly lower at day 4 and gradually decreased until day 30. On the other hand, no changes were observed after cryptorchidism. The present observations indicate that factors other than testosterone, associated with the presence of an active seminiferous epithelium, are involved in the regulation of gonadotrophin secretion. These factors do not seem to change the hypothalamic LH-RH content.

A new technique is presented to realize dual-linear polarization in four-arm sinuous antennas by simultaneously exciting all four arms of the antenna with a non-sequentially phased input. A hollow metallic cavity backing is used to obtain unidirectional radiation. The antenna exhibits a low input reflection coefficient in multiple bands across a frequency range from 1 to 4 GHz. A high level of cross-polarization rejection throughout the bandwidth of operation is demonstrated. Measurements show good agreement wtih electromagnetic simulation results.

We discuss the expected dependence of the probability transitions for 2-photon and 3-photon absorption in Helium gas on the spatial and temporal structure of the exciting radiation pulses. Regarding spatial structure, we assumed a Gaussian radial intensity distribution; we find, as expected, that the 2-photon and 3-photon processes become negligible at distances D away from the focus, where D is of the order of the beam waist FWHM. Regarding temporal structure, we compared transition probabilities for square, Gaussian and cosine squared temporal profiles; we find that for the same FWHM, Gaussian and cosine squared pulses give essentially the same transition probabilities, but the square pulses are about twice as efficient. We finally studied the effect of sharp versus smooth rise and fall edges in the light pulse; we find negligible correlation with the shape of the pulse edges, and strong correlation with the pulse FWHM, i.e., with pulse total energy, as might be expected.