Universidade Federal da Fronteira Sul

The well-established negative health outcomes of sleep deprivation, and the suggestion that availability of electricity may enable later bed times without compensating sleep extension in the morning, have stimulated interest in studying communities whose sleep pattern may resemble a pre-industrial state. Here, we describe sleep and activity in two neighbouring communities, one urban (Milange) and one rural (Tengua), in a region of Mozambique where urbanisation is an ongoing process. The two communities differ in the amount and timing of daily activity and of light exposure, with later bedtimes (≈1 h) associated with more evening and less daytime light exposure seen in the town of Milange. In contrast to previous reports comparing communities with and without electricity, sleep duration did not differ between Milange (7.28 h) and Tengua (7.23 h). Notably, calculated sleep quality was significantly poorer in rural Tengua than in Milange, and poor sleep quality was associated with a number of attributes more characteristic of rural areas, including more intense physical labour and less comfortable sleeping arrangements. Thus, whilst our data support the hypothesis that access to electricity delays sleep timing, the higher sleep quality in the urban population also suggests that some aspects of industrialisation are beneficial to sleep.

The formation of solid-solutions of iodide, bromide, and chloride provides the means to control the structure, band gap, and stability of hybrid halide perovskite semiconductors for photovoltaic applications. We report a computational investigation of the CH3NH3PbI3/CH3NH3PbBr3 alloy from density functional theory with a thermodynamic analysis performed within the generalized quasi-chemical approximation. We construct the phase diagram and identify a large miscibility gap, with a critical temperature of 343 K. The observed photoinstability in some mixed-halide solar cells is explained by the thermodynamics of alloy formation, where an initially homogeneous solution is subject to spinodal decomposition with I and Br-rich phases, which is further complicated by a wide metastable region defined by the binodal line.

Abstract 2,1,3‐Benzothiadiazole (BTD) is one of the most important nuclei used in the chemistry of photoluminescent compounds and applicable for light technology. The understanding of its properties and reactions is fundamental for the design and application of these derivatives in molecular organic electronic devices and for other technologies. As a result of their potential as constituents of organic light‐emitting diodes, solar cells, liquid crystals, dyes, photovoltaic cells, and many others, attention has been focused on BTD π‐extended derivatives with potential use in this exciting area. Herein, we describe their syntheses, properties, reactions, and selected examples of applications in light technology using by BTD (small molecules) as the core.

AbstractOropouche virus (OROV) is an important cause of arboviral illness in Latin American countries, more specifically in the Amazon region of Brazil, Venezuela and Peru, as well as in other countries such as Panama. In the past decades, the clinical, epidemiological, pathological, and molecular aspects of OROV have been published and provide the basis for a better understanding of this important human pathogen. Here, we describe the milestones in a comprehensive review of OROV epidemiology, pathogenesis, and molecular biology, including a description of the first isolation of the virus, the outbreaks during the past six decades, clinical aspects of OROV infection, diagnostic methods, genome and genetic traits, evolution, and viral dispersal.

We describe the content and properties of UV source catalogs from GALEX’s All-Sky Imaging Survey (AIS, 5σ depth ≈19.9(FUV)/20.8(NUV) mag, in the AB system) and Medium-depth Imaging Survey (MIS, 5σ depth ≈22.6(FUV)/22.7(NUV) mag), constructed by Bianchi L., et al.: Mon. Not. R. Astron. Soc. (2010, in press). The catalogs contain 65.3/12.6 million (AIS/MIS) unique UV sources with photometric error in NUV less than 0.5 mag, over 21 435(AIS)/1579(MIS) square degrees. Matched optical data from GSC-II provide additional B, R, I photometry for the brightest sources, and SDSS provides u g r i z photometry over 7325(AIS)/1103(MIS) square degrees overlap areas. We discuss statistical properties that are relevant for understanding sample selection biases and completeness, in potential science applications of these catalogs. The FUV (1344–1786 Å) and NUV (1771–2831 Å) photometry uniquely enable selection of the hottest stellar objects, in particular hot white dwarfs (WD), which are elusive at optical wavelengths because of their hot temperatures and faint luminosities. From the GALEX-SDSS matched sources we selected ∼40 000 Milky Way (MW) stars hotter than about 18 000 K (FUV-NUV < −0.13). Their density increases towards low Galactic latitudes, but drops in the MW disk due to dust extinction. The hot-WD density at different Galactic latitudes, analyzed with Milky Way models, constrains the Initial-Final Mass Relation (IFMR), relevant for understanding the yield of chemical elements from intermediate-mass stars and the chemical enrichment of the Galaxy.

OBJECTIVE To evaluate the consumption of ultra-processed foods, its associated factors, and its influence on nutrient intake in young adults. METHODS In 2004-2005, the individuals belonging to the Pelotas birth cohort of 1982 were identified for a home interview. A total of 4,297 individuals were interviewed and 4,202 individuals were included in the study (follow-up rate of 77.4%). Diet was assessed using a questionnaire on dietary intake and the percentage of daily caloric intake attributed to ultra-processed foods as well as the intake of macro- and micronutrients were estimated. The association between cohort characteristics and the consumption of ultra-processed foods was assessed using linear regression. Analysis of variance and Pearson's Chi-square test were used to evaluate the association between the quintiles of the consumption of ultra-processed food, nutrient intake and adequacy of nutrient intake, respectively. RESULTS The consumption of ultra-processed foods corresponded to 51.2% of the total caloric intake. The consumption of ultra-processed foods was higher among women, individuals with higher education, and individuals who were never poor and eutrophic. The increased consumption of ultra-processed foods was positively correlated with the consumption of fat, cholesterol, sodium, iron, calcium, and calories (p < 0.001) and was negatively correlated with the consumption of carbohydrates, protein, and dietary fiber (p < 0.001). CONCLUSIONS The high consumption of ultra-processed foods and its positive correlation with the intake of sodium, cholesterol, and fats underscores the need to perform interventions aimed at decreasing the intake of this food group.

Abstract Rice is a staple food for half of the human population. Unlike other cereals such as wheat and barley, rice plants are susceptible to cold stress, which often results in decreased productivity, especially in regions where the indica subspecies is cultivated. Low temperatures can have negative impacts on rice plants during germination, vegetative growth, and reproductive stages. Considering the expected higher frequency of extreme temperature events in the near future, cold waves could even increase the negative impacts of low temperatures in rice production. Here, we review the efforts that have been made to achieve cold tolerance in rice through breeding, the major tools used for evaluating cold tolerance in rice plants, the discovery of quantitative trait loci ( QTL s) and genes related to this tolerance, and the results obtained so far by genetic transformation of rice plants with potential cold‐tolerance genes. Although much progress has been achieved, joint efforts from breeders and plant biologists could speed up the production of cold‐tolerant rice plants, and some possible approaches are suggested.

Prognosis of patients with glioblastoma (GBM) remains very poor, thus making the development of new drugs urgent. Resveratrol (Rsv) is a natural compound that has several beneficial effects such as neuroprotection and cytotoxicity for several GBM cell lines. Here we evaluated the mechanism of action of Rsv on human GBM cell lines, focusing on the role of autophagy and its crosstalk with apoptosis and cell cycle control. We further evaluated the role of autophagy and the effect of Rsv on GBM Cancer Stem Cells (gCSCs), involved in GBM resistance and recurrence. Glioma cells treated with Rsv was tested for autophagy, apoptosis, necrosis, cell cycle and phosphorylation or expression levels of key players of these processes. Rsv induced the formation of autophagosomes in three human GBM cell lines, accompanied by an upregulation of autophagy proteins Atg5, beclin-1 and LC3-II. Inhibition of Rsv-induced autophagy triggered apoptosis, with an increase in Bax and cleavage of caspase-3. While inhibition of apoptosis or autophagy alone did not revert Rsv-induced toxicity, inhibition of both processes blocked this toxicity. Rsv also induced a S-G2/M phase arrest, accompanied by an increase on levels of pCdc2(Y15), cyclin A, E and B, and pRb (S807/811) and a decrease of cyclin D1. Interestingly, this arrest was dependent on the induction of autophagy, since inhibition of Rsv-induced autophagy abolishes cell cycle arrest and returns the phosphorylation of Cdc2(Y15) and Rb(S807/811), and levels of cyclin A, and B to control levels. Finally, inhibition of autophagy or treatment with Rsv decreased the sphere formation and the percentage of CD133 and OCT4-positive cells, markers of gCSCs. In conclusion, the crosstalk among autophagy, cell cycle and apoptosis, together with the biology of gCSCs, has to be considered in tailoring pharmacological interventions aimed to reduce glioma growth using compounds with multiple targets such as Rsv.

We present parameter-free calculations of electronic properties of InGaN, InAlN, and AlGaN alloys. The calculations are based on a generalized quasichemical approach, to account for disorder and composition effects, and first-principles calculations within the density functional theory with the LDA-1/2 approach, to accurately determine the band gaps. We provide precise results for AlGaN, InGaN, and AlInN band gaps for the entire range of compositions, and their respective bowing parameters.

An orthogonal approximation for the 8-point discrete cosine transform (DCT) is introduced. The proposed transformation matrix contains only zeros and ones; multiplications and bit-shift operations are absent. Close spectral behavior relative to the DCT was adopted as design criterion. The proposed algorithm is superior to the signed discrete cosine transform. It could also outperform state-of-the-art algorithms in low and high image compression scenarios, exhibiting at the same time a comparable computational complexity.

BACKGROUND: The increasing prevalence of overweight and obesity among the worldwide population has been associated with a range of adverse health consequences such as Type 2 diabetes and cardiovascular diseases. The metabolic syndrome (MetS) is a cluster of cardiometabolic abnormalities that occur more commonly in overweight individuals. Time-restricted feeding (TRF) is a dietary approach used for weight loss and overall health. TRF may be an option for those subjects who struggle with extreme restriction diets with foods that generally do not belong to an individual's habits. OBJECTIVE: The purpose of this study was to determine the effect of TRF on body composition and the association of weight loss with metabolic and cardiovascular risks in obese middle-aged women. METHODS: A non-randomized controlled clinical trial was performed over 3 months in obese women (TRF group, n = 20, BMI 32.53 ± 1.13 vs. Control n = 12, BMI 34.55 ± 1.20). The TRF protocol adopted was 16 h without any energy intake followed by 8 h of normal food intake. MAIN OUTCOMES AND MEASURES: Anthropometric measurements, body composition, blood biomarkers, cardiovascular risk in 30 years (CVDRisk30y), and quality of life were evaluated at baseline and after the 3 months. RESULTS: TRF was effective in reducing weight (~ 4 kg), BMI, % of body fat (%BF), waist circumference from baseline without changes in blood biomarkers associated with MetS. TRF promoted a reduction in CVDRisk30y (12%) wich was moderately correlated with %BF (r = 0.62, n = 64, p < 0.001) and %MM (r = - 0.74, n = 64, p < 0.001). CONCLUSIONS: TRF protocol reduces body weight without changes in biomarkers related to MetS. In addition, the anthropometric evaluation that predicts %BF and %MM could be used as an approach to follow individuals engaged in the TRF regimen since they correlate with cardiovascular risk.

RESUMO Objetivo Analisar a associação entre a adequação das orientações recebidas durante o pré-natal e o profissional que atendeu a gestante na maioria das consultas na Atenção Primária à Saúde. Método Participaram 3.111 puérperas que realizaram pré-natal pelo Sistema Único de Saúde no Estado de Santa Catarina em 2019, através de questionário aplicado em ambiente hospitalar até 48 horas pós-parto. Analisou-se associação entre a variável de exposição principal e covariáveis, e o desfecho segundo profissional que atendeu no pré-natal. Resultados As orientações mais frequentes foram os sinais de riscos na gestação (80,3%) e riscos de automedicação (76,9%). Observaram-se prevalências abaixo de 50% nas orientações sobre manejo adequado da amamentação (45,9%) e possibilidade de visitar a maternidade antes do parto (38,2%); ter recebido todas as orientações ao menos uma vez durante o pré-natal foi de 18,4%. Gestantes atendidas na maioria das consultas pelos profissionais médico e enfermeiro apresentaram chance 41,0% maior de adequação às orientações, em comparação com aquelas atendidas exclusivamente por médicos. Conclusões e implicações para a prática A prevalência de orientações dadas pelos profissionais de saúde às gestantes foi mais elevada quando o pré-natal foi mais compartilhado entre enfermeiros e médicos, em comparação ao atendimento majoritário por profissional de apenas uma profissão.

Several studies have shown that manipulations to the housing environment modulate susceptibility to stress in laboratory animals, mainly in rodents. Environmental enrichment (EE) is one such manipulation that promotes neuroprotection and neurogenesis, besides affecting behaviors such as drug self-administration. Zebrafish are a popular and useful animal model for behavioral neuroscience studies; however, studies evaluating the impact of housing conditions in this species are scarce. In this study, we verified the effects of EE on behavioral (novel tank test) and biochemical [cortisol and reactive oxygen species (ROS)] parameters in zebrafish submitted to unpredictable chronic stress (UCS). Consistent with our previous findings, UCS increased anxiety-like behavior, cortisol and ROS levels in zebrafish. EE for 21 or 28 days attenuated the effects induced by UCS on behavior and cortisol, and prevented the effects on ROS levels. Our findings reinforce the idea that EE exerts neuromodulatory effects across species, reducing vulnerability to stress and its biochemical impact. Also, these results indicate that zebrafish is a suitable model animal to study the behavioral effects and neurobiological mechanisms related to EE.

We present comprehensive catalogues of hot star candidates in the Milky Way (MW), selected from Galaxy Evolution Explorer (GALEX) far-UV (FUV; 1344–1786 Å) and near-UV (NUV;&#13;\n1771–2831 Å) imaging. The FUV and NUV photometry allows us to extract the hottest stellar objects, in particular hot white dwarfs (WD),which are elusive at other wavelengths because of their high temperatures and faint optical luminosities. We generated catalogues of UV sources from two GALEX’s surveys: All-Sky Imaging Survey (AIS; depth AB magnitude ~19.9/20.8 in FUV/NUV) and Medium-depth Imaging Survey (MIS; depth~22.6/22.7mag). The two catalogues (from GALEX fifth data release) contain 65.3/12.6 million (AIS/MIS) unique UV sources with error _(NUV) ≤ 0.5mag, over 21 435/1579 deg^2. We also constructed subcatalogues&#13;\nof the UV sources with matched optical photometry from Sloan Digital Sky Survey (SDSS;seventh data release): these contain 0.6/0.9 million (AIS/MIS) sources with errors ≤0.3mag in both FUV and NUV, excluding sources with multiple optical counterparts, over an area of 7325/1103 deg^2. All catalogues are available online. We then selected 28 319 (AIS)/9028 (MIS) matched sources with FUV − NUV &lt; −0.13; this colour cut corresponds to stellar&#13;\nT_(eff) hotter than ~18 000 K (the exact value varying with gravity). An additional colour cut of NUV−r &gt; 0.1 isolates binaries with largely differing T_(eff) s, and some intruding quasistellar objects (QSOs; more numerous at faint magnitudes). Available spectroscopy for a&#13;\nsubsample indicates that hot-star candidates with NUV−r &lt; 0.1 (mostly ‘single’ hot stars) have negligible contamination by non-stellar objects. We discuss the distribution of sources in the catalogues, and the effects of error and colour cuts on the samples. The density of&#13;\nhot-star candidates increases from high to low Galactic latitudes, but drops on the MW plane due to dust extinction. Our hot-star counts at all latitudes are better matched by MW models computed with an initial–final mass relation (IFMR) that favours lower final masses. The&#13;\nmodel analysis indicates that the brightest sample is likely composed of WDs located in the thin disc, at typical distances between 0.15 and 1 kpc, while the fainter sample comprises also a fraction of thick disc and halo stars. Proper motion distributions, available only for the bright&#13;\nsample (NUV &lt; 18 mag), are consistent with the kinematics of a thin-disc population.

Many compounds are used for pest control during the production and storage of rice, making it necessary to employ multiclass methods for pesticide residues determination. For this purpose, QuEChERS-based methods are very efficient, fast and accurate, and improvements in the clean-up step are important, especially for complex matrices, like cereals. In this work, different sorbents such as chitosan, florisil(®) , alumina, diatomaceous earth, graphitized carbon black, besides the commonly used primary secondary amine and octadecylsilane, were evaluated for dispersive solid-phase extraction clean-up in acetate-buffered QuEChERS method for the determination of residues of 20 representative pesticides and one metabolite in rice by liquid chromatography coupled to tandem mass spectrometry. The sorbent C18 presented the best results, however, chitosan showed similar results, and the best performance among the unconventional sorbents evaluated. The method limit of quantification, attending accuracy (70-120% recovery) and precision (RSD ≤20%) criteria, ranged from 5 to 20 μg/kg. Results showed that chitosan is an effective alternative to reduce analysis costs, maintaining the method reliability and accuracy.

In Santa Catarina, southern Brazil, family farmers modified the conventional no-till system by flattening cover crop mixtures on the soil surface as a strategy to reduce soil erosion and lower fluctuations in soil moisture and temperature, improve soil quality, and enhance weed suppression and crop performance. During 2007 and 2008, we conducted three experiments aimed at understanding the processes and mechanisms at play in successful organic conservation tillage systems (OCT), especially the underpinnings of ecological weed suppression, a key advantage of OCT systems over conventional no-till systems. Our results, as well as farmers observations, suggest that cover crops can enhance weed suppression and hence crop productivity through physical interference and allelopathy and also a host of effects on soil quality, fertility, and soil moisture that we did not measure. Results from the three trials indicate that the best cover crop mixture should include a significant proportion of rye, vetch, and fodder radish as these mixtures produce large biomass, and are readily killed by rolling forming a thick mulch sufficient to provide effective weed control in the subsequent vegetable crop.

BACKGROUND: Temozolomide (TMZ) is the most widely used drug to treat glioblastoma (GBM), which is the most common and aggressive primary tumor of the Central Nervous System and one of the hardest challenges in oncotherapy. TMZ is an alkylating agent that induces autophagy, apoptosis and senescence in GBM cells. However, therapy with TMZ increases survival after diagnosis only from 12 to 14.4 months, making the development of combined therapies to treat GBM fundamental. One candidate for GBM therapy is Resveratrol (Rsv), which has additive toxicity with TMZ in several glioma cells in vitro and in vivo. However, the mechanism of Rsv and TMZ additive toxicity, which is the aim of the present work, is not clear, especially concerning cell cycle dynamics and long term effects. METHODS: Glioma cell lines were treated with Rsv and TMZ, alone or in combinations, and the induction and the role of autophagy, apoptosis, cell cycle dynamics, protein expression and phosphorylation status were measured. We further evaluated the long term senescence induction and clonogenic capacity. RESULTS: As expected, temozolomide caused a G2 cell cycle arrest and extensive DNA damage response. Rsv did not reduced this response, even increasing pATM, pChk2 and gammaH2Ax levels, but abrogated the temozolomide-induced G2 arrest, increasing levels of cyclin B and pRb(S807/811) and reducing levels of pWee1(S642) and pCdk1(Y15). This suggests a cellular state of forced passage through G2 checkpoint despite large DNA damage, a scenario that may produce mitotic catastrophe. Indeed, the proportion of cells with high nuclear irregularity increased from 6 to 26% in 48 h after cotreatment. At a long term, a reduction in clonogenic capacity was observed, accompanied by a large induction of senescence. CONCLUSION: The presence of Rsv forces cells treated with TMZ through mitosis leading to mitotic catastrophe and senescence, reducing the clonogenic capacity of glioma cells and increasing the chronic effects of temozolomide.

Ticks are ectoparasites spread worldwide and are well known as vectors of many viruses of great importance to human and animal health. However, the viral diversity in ticks is still poorly understood, particularly in South America. Here we characterized the viral diversity present in Rhipicephalus microplus parasitizing cattle in the southern region of Brazil using metagenomics. Our study revealed the presence of viruses that had not been previously described in the region, including lihan tick virus (Phenuiviridae family) and wuhan tick virus 2 (Chuviridae family), as well as expands the biogeography of jingmen tick virus (Flaviviridae family) in Brazil. Also, we described three novel tymoviruses (Tymovirales order), named guarapuava tymovirus-like 1 to 3. We described the genomic and phylogenetic characterization of these viruses. Our study sheds light on the viral diversity of Rhipicephalus microplus in South America, and also expands the biogeography of tick viruses that were previously described only in Asia.

A pandemia de coronavírus que atingiu o mundo no final de 2019 segue batendo recordes de novos casos e óbitos relacionados à doença. As orientações para o manejo clínico dos pacientes infectados e a prevenção de novos casos estão centradas nas medidas de controle dos sintomas, hábitos de higiene, isolamento social e diminuição da aglomeração de pessoas. Tal fato forçou uma mudança no modo como os serviços de saúde prestam cuidados, protagonizando a incorporação de novas tecnologias em saúde. Assim, este Ensaio objetiva compilar e analisar algumas experiências de uso das tecnologias digitais em saúde, para minimizar os impactos da COVID-19. Identificou-se o desenvolvimento de soluções tecnológicas de manejo clínico do paciente, diagnóstico por imagem, uso de inteligência artificial para análise de riscos, aplicativos de geolocalização, ferramentas para análise de dados e relatórios, autodiagnóstico e, inclusive, de orientação à tomada de decisão. A grande maioria das iniciativas listadas tem sido eficaz na minimização dos impactos da COVID-19 nos sistemas de saúde, de modo que visa à diminuição da aglomeração de pessoas e assim facilita o acesso aos serviços, bem como contribui para a incorporação de novas práticas e modos de cuidar, em saúde.

The adoption of integrated pest management (IPM) in soybean is a great example of how this technology is essential to guarantee crop sustainability and productivity. In Brazil, up to 1970, soybean was cultivated with the use of pesticides applied without the adoption of any economic threshold to base this decision. As a consequence, a six-insecticide spraying per crop season used to be the average insecticide dosage. With the introduction of Soybean-IPM, the use of pesticides was reduced to approximately two applications per season. Comparing these two contrasting situations, the advantages of using IPM methods cannot be ignored, since they are economically and mostly environmentally feasible. Despite the benefits provided by Soybean- -IPM, this program was abandoned in Brazil, and its principles were almost forgotten, and, as a consequence, insecticide use increased. The success and failures of Soybean-IPM in Brazil will be further analyzed in this review in an attempt to point out how safe this technology is and whether there is any risk in fully adopting this approach.