Cloning and expression in Escherichia coli of a human cDNA encoding the DNA repair protein N-methylpurine-DNA glycosylase

Chakravarti, Debabrata; C. Ibeanu, Gordon; Tano, Keizo; Mitra, Sankar

doi:10.1016/s0021-9258(18)98467-x

Public

Cloning and expression in Escherichia coli of a human cDNA encoding the DNA repair protein N-methylpurine-DNA glycosylase

Published in Journal of Biological Chemistry • Aug 1, 1991

Authors:

Debabrata Chakravarti

Gordon C. Ibeanu

Keizo Tano

Abstract

A 871-base pair cDNA encoding the human N-methylpurine-DNA glycosylase (MPG) was cloned from a HeLa S3 cDNA expression library in a pUC vector by phenotypic screening of MPG-negative (tag- alkA-) Escherichia coli cells exposed to methylmethane sulfonate. The active MPG is expressed as a 31-kDa fusio...

Subject

Complementary DNA

Escherichia coli

Cloning (programming)

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