On the cloning of eukaryotic total poly(A)-RNA populations in Escherichia coli.

V. Norgard, Michael; J. Tocci, Michael; J. Monahan, John

doi:10.1016/s0021-9258(19)43881-7

Public

On the cloning of eukaryotic total poly(A)-RNA populations in Escherichia coli.

Published in Journal of Biological Chemistry • Aug 1, 1980

Authors:

Michael V. Norgard

Michael J. Tocci

John J. Monahan

Abstract

A total clone bank of cDNAs synthesized from mouse liver poly(A)-RNA was constructed in Escherichia coli K-12 RR1 using the plasmid pBR322. Sequences of cDNAs were inserted into the PST-I site of pBR322 by the "G-C-tailing" method. Bulk preparations of these cDNA sequences were obtained by treatment...

Subject

Biology

Complementary DNA

PBR322

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