Mitochondrial precursor protein. Effects of 70-kilodalton heat shock protein on polypeptide folding, aggregation, and import competence.

P. Sheffield, William; C Shore, G; K. Randall, Stephen

doi:10.1016/s0021-9258(19)38558-8

Public

Mitochondrial precursor protein. Effects of 70-kilodalton heat shock protein on polypeptide folding, aggregation, and import competence.

Published in Journal of Biological Chemistry • Jul 1, 1990

Authors:

William P. Sheffield

G C Shore

Stephen K. Randall

Abstract

A hybrid precursor protein constructed by fusing the mitochondrial matrix-targeting signal of rat preornithine carbamyl transferase to murine cytosolic dihydrofolate reductase (designated pO-DHFR) was expressed in Escherichia coli. Following purification under denaturing conditions, pO-DHFR was capa...

Subject

Reticulocyte

Cytosol

Biochemistry

Finding related papers...

Discussions

(0)

No comments yet

Be the first to share your thoughts!