Rat pro-inflammatory cytokine and cytokine related mRNA quantification by real-time polymerase chain reaction using SYBR green

Peinnequin, André; Mouret, Catherine; Birot, Olivier; Alonso, Antonia; Mathieu, Jacques; Clarençon, Didier; Agay, Diane; Chancerelle, Yves; Multon, E.

doi:10.1186/1471-2172-5-3

Public

Rat pro-inflammatory cytokine and cytokine related mRNA quantification by real-time polymerase chain reaction using SYBR green

Published in BMC Immunology • Feb 5, 2004

Authors:

André Peinnequin

Catherine Mouret

Olivier Birot

Abstract

BACKGROUND: Cytokine mRNA quantification is widely used to investigate cytokine profiles, particularly in small samples. Real-time polymerase chain reaction is currently the most reliable method of quantifying low-level transcripts such as cytokine and cytokine receptor mRNAs. This accurate techniqu...

Subject

Cytokine

Real-time polymerase chain reaction

Biology

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