Tissue Preservation and Total DNA Extraction form Fish Stored at Ambient Temperature Using Buffers Containing High Concentration of Urea

Asahida, Takashi; Kobayashi, Takanori; Saitoh, Kenji; Nakayama, Ichiro

doi:10.2331/fishsci.62.727

Public

Tissue Preservation and Total DNA Extraction form Fish Stored at Ambient Temperature Using Buffers Containing High Concentration of Urea

Published in Fisheries Science • Jan 1, 1996

Authors:

Takashi Asahida

Takanori Kobayashi

Kenji Saitoh

Abstract

We have developed a high concentration urea containing buffer (TNES-Urea: 6 or 8M urea; 10mM Tris-HCl, pH 7.5; 125mM NaCl; 10mM EDTA; 1% SDS) for DNA extraction by modifying the cell lysis buffer for DNA isolation, and we found this buffer is suitable for long-term preservation of tissue samples fro...

Subject

DNA extraction

Clupea

Urea

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