Molecular Sequencing and Modeling of <i>Neobellieria bullata</i> Trypsin

Borovsky, Dov; Janssen, Ine; Vanden Broeck, Jozef; Huybrechts, Roger; Verhaert, Peter; L. De Bondt, Hendrik; Bylemans, D.; De Loof, Arnold

doi:10.1111/j.1432-1033.1996.0279n.x

Public

Molecular Sequencing and Modeling of Neobellieria bullata Trypsin

Published in European Journal of Biochemistry • Apr 1, 1996

Authors:

Dov Borovsky

Ine Janssen

Jozef Vanden Broeck

Abstract

Trypsin mRNA from the grey fleshfly (Neobellieria bullata) was reversed transcribed and amplified by means of PCR. Two cDNA species of 600 bp and 800 bp were cloned and sequenced. The 3' end of the gene (300 bp) was amplified by means of the rapid-amplification-of-cDNA-ends method, cloned and sequen...

Subject

Trypsin

Complementary DNA

Molecular biology

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