Transformation of NIH 3T3 cells with cloned fragments of human cytomegalovirus strain AD169.

JA, Nelson; B, Fleckenstein; DA, Galloway; JK, McDougall

doi:10.1128/jvi.43.1.83-91.1982

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Transformation of NIH 3T3 cells with cloned fragments of human cytomegalovirus strain AD169.

Shared by NobleBlocks on Jul 1, 1982 • 12:00 AM UTC

Authors:

Nelson JA

Fleckenstein B

Galloway DA

Abstract

NIH 3T3 cells were transfected with restriction endonuclease and cloned human cytomegalovirus DNA fragments to identify the transforming region(s). Cleavage of human cytomegalovirus strain AD169 DNA with XbaI and HindIII left a transforming region intact whereas EcoRI inactivated this function. Tran...

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