Barcoding the kingdom Plantae: new <scp>PCR</scp> primers for <i><scp>ITS</scp></i> regions of plants with improved universality and specificity

Cheng, Tao; Xu, Chao; Li, Lei; Li, Changhao; Zhang, Yu; Zhou, Shiliang

doi:10.1111/1755-0998.12438

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Barcoding the kingdom Plantae: new PCR primers for ITS regions of plants with improved universality and specificity

Published in Molecular Ecology Resources • Jun 18, 2015

Authors:

Tao Cheng

Chao Xu

Lei Li

Abstract

The internal transcribed spacer (ITS) of nuclear ribosomal DNA is one of the most commonly used DNA markers in plant phylogenetic and DNA barcoding analyses, and it has been recommended as a core plant DNA barcode. Despite this popularity, the universality and specificity of PCR primers for the ITS ...

Subject

Biology

Genetics

Microbiology

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