Polymerase Chain Reaction Amplification of a Repetitive DNA Sequence Specific for Mycobacterium tuberculosis

D. Eisenach, Kathleen; Donald Cave, M.; H. Bates, Joseph; T. Crawford, Jack

doi:10.1093/infdis/161.5.977

Public

Polymerase Chain Reaction Amplification of a Repetitive DNA Sequence Specific for Mycobacterium tuberculosis

Published in The Journal of Infectious Diseases • May 1, 1990

Authors:

Kathleen D. Eisenach

M. Donald Cave

Joseph H. Bates

Abstract

A segment of DNA repeated in the chromosome of Mycobacterium tuberculosis was sequenced and used as a target for amplification using polymerase chain reaction (PCR). The sequences of the primers (5' to 3') were CCTGCGAGCGTAGGCGTCGG and CTCGTCCAGCGCCGCTTCGG, and a temperature of 68 degrees C was used...

Subject

Polymerase chain reaction

Mycobacterium tuberculosis

Sequence (biology)

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