Sequential Treatment of SH‐SY5Y Cells with Retinoic Acid and Brain‐Derived Neurotrophic Factor Gives Rise to Fully Differentiated, Neurotrophic Factor‐Dependent, Human Neuron‐Like Cells

Encinas, Mario; Iglesias, Montse; Liu, Yuhui; Wang, Hongyin; Muhaisen, Ashraf; Ceña, Valentı́n; Gallego, Carme; X. Comella, Joan

doi:10.1046/j.1471-4159.2000.0750991.x

Public

Sequential Treatment of SH‐SY5Y Cells with Retinoic Acid and Brain‐Derived Neurotrophic Factor Gives Rise to Fully Differentiated, Neurotrophic Factor‐Dependent, Human Neuron‐Like Cells

Published in Journal of Neurochemistry • Sep 1, 2000

Authors:

Mario Encinas

Montse Iglesias

Yuhui Liu

Abstract

A rapid and simple procedure is presented to obtain nearly pure populations of human neuron-like cells from the SH-SY5Y neuroblastoma cell line. Sequential exposure of SH-SY5Y cells to retinoic acid and brain-derived neurotrophic factor in serum-free medium yields homogeneous populations of cells wi...

Subject

Neurotrophic factors

Biology

Ciliary neurotrophic factor

Finding related papers...

Discussions

(0)

No comments yet

Be the first to share your thoughts!