Mitochondrial membrane potential measurement in rat cerebellar neurons by flow cytometry

X. Sureda, Francesc; Escubedo, Elena; Gabriel, Cec�lia; Comas, Jaume; Camarasa, Jorge; Camins, Antoni

doi:10.1002/(sici)1097-0320(19970501)28:1<74::aid-cyto9>3.0.co;2-h

Public

Mitochondrial membrane potential measurement in rat cerebellar neurons by flow cytometry

Published in Cytometry • May 1, 1997

Authors:

Francesc X. Sureda

Elena Escubedo

Cec�lia Gabriel

Abstract

Mitochondrial membrane potential (MMP) in dissociated rat cerebellar neurons was measured using rhodamine 123 (Rh 123) as fluorescent dye, and flow cytometry. Dye distribution was studied by confocal scanning microscopy. Propidium iodide (PI)-marked cells (dead cells) were not stained by Rh 123, whi...

Subject

Rhodamine 123

Propidium iodide

Flow cytometry

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