Simple and reliable multiplex PCR assay for detection of blaTEM, blaSHV and blaOXA-1 genes in Enterobacteriaceae

Colom, K.; PÃ©rez, Javier; Alonso, Rodrigo; FernÃ¡ndez-Aranguiz, Agueda; LariÃ±o, Eva; n Cisterna, RamÃ

doi:10.1016/s0378-1097(03)00306-9

Public

Simple and reliable multiplex PCR assay for detection of blaTEM, blaSHV and blaOXA-1 genes in Enterobacteriaceae

Published in FEMS Microbiology Letters • Jun 1, 2003

Authors:

K. Colom

Javier PÃ©rez

Rodrigo Alonso

Abstract

Third-generation cephalosporin resistance is often mediated by TEM- and SHV-type beta-lactamases in Enterobacteriaceae. TEM-type and OXA-1 enzymes are the major plasmid-borne beta-lactamases implicated in amoxicillin-clavulanic acid resistance in Escherichia coli isolates. We have developed a rapid ...

Subject

Enterobacteriaceae

Multiplex polymerase chain reaction

Biology

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