Improved Gateway Binary Vectors: High-Performance Vectors for Creation of Fusion Constructs in Transgenic Analysis of Plants

Nakagawa, Tsuyoshi; Suzuki, Takamasa; Murata, Satoko; Nakamura, Shinya; Hino, Takeshi; Maeo, Kenichiro; Tabata, Ryo; Kawai, Tsutae; Tanaka, Katsunori; Niwa, Yasuo; Watanabe, Yuichiro; Nakamura, Kenzo; Kimura, Tetsuya; Ishiguro, Sumie

doi:10.1271/bbb.70216

Public

Improved Gateway Binary Vectors: High-Performance Vectors for Creation of Fusion Constructs in Transgenic Analysis of Plants

Published in Bioscience Biotechnology and Biochemistry • Aug 23, 2007

Authors:

Tsuyoshi Nakagawa

Takamasa Suzuki

Satoko Murata

Abstract

We made a series of improved Gateway binary vectors (pGWBs) for plant transformation. Fifteen different reporters and tags, sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7, and TAP, were employed. Some vectors carry the 2x35S-Omega promoter for higher-level express...

Subject

Transformation (genetics)

Kanamycin

Plasmid

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