Development of an Internally Controlled Real-Time Reverse Transcriptase PCR Assay for Pan-Dengue Virus Detection and Comparison of Four Molecular Dengue Virus Detection Assays

J. Waggoner, Jesse; Abeynayake, Janaki; K. Sahoo, Malaya; Gresh, Lionel; Téllez, Yolanda; González, Karla; Ballesteros, Gabriela; Balmaseda, Ángel; Karunaratne, Kumudu; Harris, Eva; A. Pinsky, Benjamin

doi:10.1128/jcm.00548-13

Public

Development of an Internally Controlled Real-Time Reverse Transcriptase PCR Assay for Pan-Dengue Virus Detection and Comparison of Four Molecular Dengue Virus Detection Assays

Published in Journal of Clinical Microbiology • May 2, 2013

Authors:

Jesse J. Waggoner

Janaki Abeynayake

Malaya K. Sahoo

Abstract

A number of diagnostic tests are available for dengue virus (DENV) detection, including a variety of nucleic acid amplification tests (NAATs). However, reports describing a direct comparison of different NAATs have been limited. In this study, we report the design of an internally controlled real-ti...

Subject

Dengue virus

Virology

Multiplex

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