Expanded dynamic range of fluorescent indicators for Ca <sup>2+</sup> by circularly permuted yellow fluorescent proteins

Nagai, Takeharu; Yamada, Shuichi; Tominaga, Takashi; Ichikawa, Michinori; Miyawaki, Atsushi

doi:10.1073/pnas.0400417101

Public

Expanded dynamic range of fluorescent indicators for Ca 2+ by circularly permuted yellow fluorescent proteins

Published in Proceedings of the National Academy of Sciences • Jul 9, 2004

Authors:

Takeharu Nagai

Shuichi Yamada

Takashi Tominaga

Abstract

Fluorescence resonance energy transfer (FRET) technology has been used to develop genetically encoded fluorescent indicators for various cellular functions. Although most indicators have cyan- and yellow-emitting fluorescent proteins (CFP and YFP) as FRET donor and acceptor, their poor dynamic range...

Subject

Förster resonance energy transfer

Yellow fluorescent protein

Fluorescence

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