Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.

F, Colbere-Garapin; S, Chousterman; F, Horodniceanu; P, Kourilsky; AC, Garapin

doi:10.1073/pnas.76.8.3755

Public

Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.

Shared by NobleBlocks on Aug 1, 1979 • 12:00 AM UTC

Authors:

Colbere-Garapin F

Chousterman S

Horodniceanu F

Abstract

A herpes simplex virus DNA fragment that is produced by digestion with BamHI endonuclease and carries the thymidine kinase (TK; ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21) gene has been cloned in Escherichia coli. A recombinat plasmid, pFG5, has been analyzed extensively and a detailed restric...

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