Semi-rational approach for converting a GH1  -glycosidase into a  -transglycosidase

Tezé, David; Hendrickx, Jan; Czjzek, Mirjam; Ropartz, David; Sanejouand, Yves‐Henri; Tran, Vinh; Tellier, Charles; Dion, Michel

doi:10.1093/protein/gzt057

Public

Semi-rational approach for converting a GH1 -glycosidase into a -transglycosidase

Published in Protein Engineering Design and Selection • Nov 27, 2013

Authors:

David Tezé

Jan Hendrickx

Mirjam Czjzek

Abstract

A large number of retaining glycosidases catalyze both hydrolysis and transglycosylation reactions, but little is known about what determines the balance between these two activities (transglycosylation/hydrolysis ratio). We previously obtained by directed evolution the mutants F401S and N282T of Th...

Subject

Thermus thermophilus

Glycoside hydrolase

Hydrolysis

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