Fluorescence Resonance Energy Transfer Between Quantum Dot Donors and Dye-Labeled Protein Acceptors

R. Clapp, Aaron; L. Medintz, Igor; Matthew Mauro, J.; Fisher, Brent; G. Bawendi, Moungi; Mattoussi, Hedi

doi:10.1021/ja037088b

Public

Fluorescence Resonance Energy Transfer Between Quantum Dot Donors and Dye-Labeled Protein Acceptors

Published in Journal of the American Chemical Society • Dec 13, 2003

Authors:

Aaron R. Clapp

Igor L. Medintz

J. Matthew Mauro

Abstract

We used luminescent CdSe-ZnS core-shell quantum dots (QDs) as energy donors in fluorescent resonance energy transfer (FRET) assays. Engineered maltose binding protein (MBP) appended with an oligohistidine tail and labeled with an acceptor dye (Cy3) was immobilized on the nanocrystals via a noncovale...

Subject

Förster resonance energy transfer

Chemistry

Quantum dot

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