From β‐glucanase to β‐glucansynthase: glycosyl transfer to α‐glycosyl fluorides catalyzed by a mutant endoglucanase lacking its catalytic nucleophile

Malet, Carles; Planas, Antoni

doi:10.1016/s0014-5793(98)01448-3

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From β‐glucanase to β‐glucansynthase: glycosyl transfer to α‐glycosyl fluorides catalyzed by a mutant endoglucanase lacking its catalytic nucleophile

Shared by NobleBlocks on Nov 27, 1998 • 12:00 AM UTC

Authors:

Carles Malet

Antoni Planas

Abstract

Removal of the catalytic nucleophile Glu134 of the retaining 1,3‐1,4‐β‐glucanase from Bacillus licheniformis by mutation to alanine yields an enzyme with no glycosidase activity. The mutant is able to catalyze the regio‐ and stereospecific glycosylation of α‐laminaribiosyl fluoride with different gl...

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