Construction of biologically functional bacterial plasmids in vitro.
Published in Proceedings of the National Academy of Sciences of the United States of America • Nov 1, 1973
NobleIDNI8P00W57R99S11
Authors:,,
S N Cohen
A C Chang
H W Boyer
Abstract
The construction of new plasmid DNA species by in vitro joining of restriction endonuclease-generated fragments of separate plasmids is described. Newly constructed plasmids that are inserted into Escherichia coli by transformation are shown to be biologically functional replicons that possess genet...
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