DNA copy number concentration measured by digital and droplet digital quantitative PCR using certified reference materials

Corbisier, Philippe; Pinheiro, Leonardo; Stephane, Mazoua; Kortekaas, Anne-Marie; Yan Jenny Chung, Pui; Gerganova, Tsvetelina; Roebben, Gert; Emons, Hendrik; R. Emslie, Kerry

doi:10.1007/s00216-015-8458-z

Public

DNA copy number concentration measured by digital and droplet digital quantitative PCR using certified reference materials

Published in Analytical and Bioanalytical Chemistry • Jan 19, 2015

Authors:

Philippe Corbisier

Leonardo Pinheiro

Mazoua Stephane

Abstract

The value assignment for properties of six certified reference materials (ERM-AD623a-f), each containing a plasmid DNA solution ranging from 1 million to 10 copies per μL, by using digital PCR (dPCR) with the BioMark™ HD System (Fluidigm) has been verified by applying droplet digital PCR (ddPCR) usi...

Subject

Digital polymerase chain reaction

Volume (thermodynamics)

Analytical Chemistry (journal)

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