Single-Reaction, Multiplex, Real-Time RT-PCR for the Detection, Quantitation, and Serotyping of Dengue Viruses

J. Waggoner, Jesse; Abeynayake, Janaki; K. Sahoo, Malaya; Gresh, Lionel; Téllez, Yolanda; González, Karla; Ballesteros, Gabriela; Pierro, Anna; Gaibani, Paolo; P. Guo, Frances; Sambri, Vittorio; Balmaseda, Ángel; Karunaratne, Kumudu; Harris, Eva; A. Pinsky, Benjamin

doi:10.1371/journal.pntd.0002116

Public

Single-Reaction, Multiplex, Real-Time RT-PCR for the Detection, Quantitation, and Serotyping of Dengue Viruses

Published in PLoS neglected tropical diseases • Apr 18, 2013

Authors:

Jesse J. Waggoner

Janaki Abeynayake

Malaya K. Sahoo

Abstract

BACKGROUND: Dengue fever results from infection with one or more of four different serotypes of dengue virus (DENV). Despite the widespread nature of this infection, available molecular diagnostics have significant limitations. The aim of this study was to develop a multiplex, real-time, reverse tra...

Subject

Dengue virus

Virology

Serotype

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