Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals

D. Sarker, Satyajit; Nahar, Lutfun; Kumarasamy, Yashodharan

doi:10.1016/j.ymeth.2007.01.006

Public

Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals

Published in Methods • Jun 7, 2007

Authors:

Satyajit D. Sarker

Lutfun Nahar

Yashodharan Kumarasamy

Abstract

The resazurin assay utilising microtitre-plate, described by Drummond and Waigh in 2000, has been modified to achieve more accuracy in the determination of the minimum inhibitory concentration (MIC) values of natural products, including crude extracts, chromatographic fractions or purified compounds...

Subject

Resazurin

Minimum inhibitory concentration

Chromatography

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