Imaging cells and extracellular matrix <i>in vivo</i> by using second-harmonic generation and two-photon excited fluorescence

Zoumi, Aikaterini; T. Yeh, Alvin; J. Tromberg, Bruce

doi:10.1073/pnas.172368799

Public

Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence

Published in Proceedings of the National Academy of Sciences • Aug 12, 2002

Authors:

Aikaterini Zoumi

Alvin T. Yeh

Bruce J. Tromberg

Abstract

Multiphoton microscopy relies on nonlinear light-matter interactions to provide contrast and optical sectioning capability for high-resolution imaging. Most multiphoton microscopy studies in biological systems have relied on two-photon excited fluorescence (TPEF) to produce images. With increasing a...

Subject

Two-photon excitation microscopy

Autofluorescence

Second-harmonic generation

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