Evaluation of Extraction Methodologies for Corn Kernel (<i>Zea mays</i>) DNA for Detection of Trace Amounts of Biotechnology-Derived DNA

J. Holden, Marcia; R. Blasic, Joseph; J. Bussjaeger, Louis; Kao, Chuan; A. Shokere, Luke; C. Kendall, Donald; Freese, Larry; Jenkins, Gail

doi:10.1021/jf0211130

Public

Evaluation of Extraction Methodologies for Corn Kernel (Zea mays) DNA for Detection of Trace Amounts of Biotechnology-Derived DNA

Published in Journal of Agricultural and Food Chemistry • Mar 27, 2003

Authors:

Marcia J. Holden

Joseph R. Blasic

Louis J. Bussjaeger

Abstract

Sensitive and accurate testing for trace amounts of biotechnology-derived DNA from plant material requires pure, high-quality genomic DNA as template for subsequent amplification using the polymerase chain reaction (PCR). Six methodologies were evaluated for extracting DNA from ground corn kernels s...

Subject

genomic DNA

DNA

DNA extraction

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