A Versatile Nanotrap for Biochemical and Functional Studies with Fluorescent Fusion Proteins

Rothbauer, Ulrich; Zolghadr, Kourosh; Muyldermans, Serge; Schepers, Aloys; Cristina Cardoso, M.; Leonhardt, Heinrich

doi:10.1074/mcp.m700342-mcp200

Public

A Versatile Nanotrap for Biochemical and Functional Studies with Fluorescent Fusion Proteins

Published in Molecular & Cellular Proteomics • Oct 21, 2007

Authors:

Ulrich Rothbauer

Kourosh Zolghadr

Serge Muyldermans

Abstract

Green fluorescent proteins (GFPs) and variants thereof are widely used to study protein localization and dynamics. We engineered a specific binder for fluorescent proteins based on a 13-kDa GFP binding fragment derived from a llama single chain antibody. This GFP-binding protein (GBP) can easily be ...

Subject

Green fluorescent protein

Fusion protein

Fluorescence

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