Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

Baret, Jean‐Christophe; J. Miller, Oliver; Taly, Valérie; Ryckelynck, Michaël; El-Harrak, Abdeslam; Frenz, Lucas; Rick, Christian; L. Samuels, Michael; Brian Hutchison, J.; J. Agresti, Jeremy; R. Link, Darren; A. Weitz, David; D. Griffiths, Andrew

doi:10.1039/b902504a

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Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

Published in Lab on a Chip • Jan 1, 2009

Authors:

Jean‐Christophe Baret

Oliver J. Miller

Valérie Taly

Abstract

We describe a highly efficient microfluidic fluorescence-activated droplet sorter (FADS) combining many of the advantages of microtitre-plate screening and traditional fluorescence-activated cell sorting (FACS). Single cells are compartmentalized in emulsion droplets, which can be sorted using diele...

Subject

Microfluidics

Cell sorting

Fluorescence

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