Structural and functional polarity of canalicular and basolateral plasma membrane vesicles isolated in high yield from rat liver.

PJ, Meier; ES, Sztul; A, Reuben; JL, Boyer

doi:10.1083/jcb.98.3.991

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Structural and functional polarity of canalicular and basolateral plasma membrane vesicles isolated in high yield from rat liver.

Shared by NobleBlocks on Mar 1, 1984 • 12:00 AM UTC

Authors:

Meier PJ

Sztul ES

Reuben A

Abstract

A method has been developed for routine high yield separation of canalicular (cLPM) from basolateral (blLPM) liver plasma membrane vesicles of rat liver. Using a combination of rate zonal floatation (TZ-28 zonal rotor, Sorvall) and high speed centrifugation through discontinuous sucrose gradients, 9...

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