Flavonoids: A class of modulators with bifunctional interactions at vicinal ATP- and steroid-binding sites on mouse P-glycoprotein

Conseil, Gwenaëlle; Baubichon-Cortay, Helene; Dayan, Guila; Jault, Jean‐Michel; Barron, Denís; Di Pietro, Attilio

doi:10.1073/pnas.95.17.9831

Public

Flavonoids: A class of modulators with bifunctional interactions at vicinal ATP- and steroid-binding sites on mouse P-glycoprotein

Published in Proceedings of the National Academy of Sciences • Aug 18, 1998

Authors:

Gwenaëlle Conseil

Helene Baubichon-Cortay

Guila Dayan

Abstract

A hexahistidine-tagged C-terminal nucleotide-binding domain (H6-NBD2) from mouse P-glycoprotein was designed, overexpressed, and purified as a highly soluble recombinant protein. Intrinsic fluorescence of its single tryptophan residue allowed monitoring of high-affinity binding of 2'(3')-N-methylant...

Subject

Binding site

Chemistry

Biochemistry

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