Identification of Free-Living Amoebae and Amoeba-Associated Bacteria from Reservoirs and Water Treatment Plants by Molecular Techniques

García, Alicia; Goñi, Pilar; Cieloszyk, Joanna; Teresa Fernández‐Rodrigo, María; Calvo-Beguería, Laura; Rubio, Encarnacion; F. Fillat, Marı́a; Luisa Peleato, María; Clavel, Antonio

doi:10.1021/es400160k

Public

Identification of Free-Living Amoebae and Amoeba-Associated Bacteria from Reservoirs and Water Treatment Plants by Molecular Techniques

Published in Environmental Science & Technology • Feb 27, 2013

Authors:

Alicia García

Pilar Goñi

Joanna Cieloszyk

Abstract

The occurrence of free-living amoebae (FLA) was investigated in 83 water samples from reservoirs and water treatment plants, with culture positive in 64 of them (77.1%). Polymerase chain reaction (PCR) of partial 18S rRNA gene and ITS region was performed in order to identify amoeba isolates, and th...

Subject

Acanthamoeba

Naegleria

Microbiology

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