Identification of free-living amoebae and amoeba-associated bacteria from reservoirs and water treatment plants by molecular techniques.

Garcia, Alicia; Goñi, Pilar; Cieloszyk, Joanna; Teresa Fernandez, Maria; Calvo-Beguería, Laura; Rubio, Encarnacion; Francisca Fillat, Maria; Luisa Peleato, Maria; Clavel, Antonio

doi:10.1021/es400160k

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Identification of free-living amoebae and amoeba-associated bacteria from reservoirs and water treatment plants by molecular techniques.

Shared by NobleBlocks on Apr 2, 2013 • 12:00 AM UTC

Authors:

Alicia Garcia

Pilar Goñi

Joanna Cieloszyk

Abstract

The occurrence of free-living amoebae (FLA) was investigated in 83 water samples from reservoirs and water treatment plants, with culture positive in 64 of them (77.1%). Polymerase chain reaction (PCR) of partial 18S rRNA gene and ITS region was performed in order to identify amoeba isolates, and th...

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