Effects of heparin on polymerase chain reaction for blood white cells

Yokota, Masaharu; Tatsumi, Noriyuki; Nathalang, Oytip; Yamada, Tetsuaki; Tsuda, Izumi

doi:10.1002/(sici)1098-2825(1999)13:3<133::aid-jcla8>3.0.co;2-0

Public

Effects of heparin on polymerase chain reaction for blood white cells

Published in Journal of Clinical Laboratory Analysis • Jan 1, 1999

Authors:

Masaharu Yokota

Noriyuki Tatsumi

Oytip Nathalang

Abstract

Polymerase chain reaction (PCR) has been used with increasing frequency to diagnose infectious and genetic diseases. In this study, the effects of heparin on PCR were investigated, because heparinized blood may sometimes be used in PCR studies. HLA-DQA1 gene amplification was used as a model. PCR wa...

Subject

Polymerase chain reaction

Heparin

Molecular biology

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