Unfolding free energy changes determined by the linear extrapolation method. 1. Unfolding of phenylmethanesulfonyl .alpha.-chymotrypsin using different denaturants

M. Santoro, Marcelo; Wayne Bolen, D.

doi:10.1021/bi00421a014

Public

Unfolding free energy changes determined by the linear extrapolation method. 1. Unfolding of phenylmethanesulfonyl .alpha.-chymotrypsin using different denaturants

Published in Biochemistry • Oct 18, 1988

Authors:

Marcelo M. Santoro

D. Wayne Bolen

Abstract

Characteristics and properties of the unfolding free energy change, delta G degrees N-U, as determined by the linear extrapolation method are assessed for the unfolding of phenylmethanesulfonyl chymotrypsin (PMS-Ct). Difference spectral measurements at 293 nm were used to define PMS-Ct unfolding bro...

Subject

Alpha-chymotrypsin

Chymotrypsin

Extrapolation

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