Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure

Daniel Gietz, R.; H. Schiestl, Robert; Willems, Andrew; A. Woods, Robin

doi:10.1002/yea.320110408

Public

Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure

Published in Yeast • Apr 15, 1995

Authors:

R. Daniel Gietz

Robert H. Schiestl

Andrew Willems

Abstract

An improved lithium acetate (LiAc)/single-stranded DNA (SS-DNA)/polyethylene glycol (PEG) protocol which yields > 1 x 10(6) transformants/micrograms plasmid DNA and the original protocol described by Schiestl and Gietz (1989) were used to investigate aspects of the mechanism of LiAc/SS-DNA/PEG trans...

Subject

Plasmid

Transformation (genetics)

DNA

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