Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure

Daniel Gietz, R.; H. Schiestl, Robert; R. Willems, Andrew; A. Woods, Robin

doi:10.1002/yea.320110408

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Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure

Shared by NobleBlocks on Apr 15, 1995 • 12:00 AM UTC

Authors:

R. Daniel Gietz

Robert H. Schiestl

Andrew R. Willems

Abstract

Abstract An improved lithium acetate (LiAc)/single‐stranded DNA (SS‐DNA)/polyethylene glycol (PEG) protocol which yields >1 × 10 6 transformants/μg plasmid DNA and the original protocol described by Schiestl and Gietz (1989) were used to investigate aspects of the mechanism of LiAc/SS‐DNA/PEG transf...

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