Comprehensive Step‐by‐Step Engineering of an (<i>R</i>)‐Hydroxynitrile Lyase for Large‐Scale Asymmetric Synthesis

Glieder, Anton; Weís, Roland; Skranc, Wolfgang; Poechlauer, Peter; Dreveny, Ingrid; Majer, Sandra; Wubbolts, Marcel; Schwab, Helmut; Gruber, Karl

doi:10.1002/anie.200352141

Public

Comprehensive Step‐by‐Step Engineering of an (R)‐Hydroxynitrile Lyase for Large‐Scale Asymmetric Synthesis

Published in Angewandte Chemie International Edition • Oct 8, 2003

Authors:

Anton Glieder

Roland Weís

Wolfgang Skranc

Abstract

A custom-made enzyme: The gene and protein sequence of a new (R)-hydroxynitrile lyase from Prunus amygdalus was cloned and engineered for recombinant production on a large scale. The enzyme is stable and active at low pH values. A rationally designed active-site mutant now permits the rapid and ster...

Subject

Steric effects

Protein engineering

Chemistry

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