Efficient isolation and mapping of <i>Arabidopsis thaliana</i> T‐DNA insert junctions by thermal asymmetric interlaced PCR

Liu, Yao‐Guang; Mitsukawa, Norihiro; Oosumi, Teruko; F. Whittier, Robert

doi:10.1046/j.1365-313x.1995.08030457.x

Public

Efficient isolation and mapping of Arabidopsis thaliana T‐DNA insert junctions by thermal asymmetric interlaced PCR

Published in The Plant Journal • Sep 1, 1995

Authors:

Yao‐Guang Liu

Norihiro Mitsukawa

Teruko Oosumi

Abstract

Thermal asymmetric interlaced (TAIL-) PCR is an efficient technique for amplifying insert ends from yeast artificial chromosome (YAC) and P1 clones. Highly specific amplification is achieved without resort to complex manipulations before or after PCR. The adaptation of this method for recovery and m...

Subject

Insert (composites)

Biology

Genetics

Research Assistant

AI chat, annotations, notes & similar papers

Finding related papers...

Discussions

(0)

No comments yet

Be the first to share your thoughts!