Improved software detection and extraction of ITS1 and <scp>ITS</scp> 2 from ribosomal <scp>ITS</scp> sequences of fungi and other eukaryotes for analysis of environmental sequencing data

Bengtsson‐Palme, Johan; Ryberg, Martin; Hartmann, Martin; Branco, Sara; Wang, Zheng; Godhe, Anna; De Wit, Pierre; Sánchez‐García, Marisol; Ebersberger, Ingo; Sousa, Filipe; S. Amend, Anthony; Jumpponen, Ari; Unterseher, Martin; Kristiansson, Erik; Abarenkov, Kessy; Bertrand, Yann; Sanli, Kemal; Martin Eriksson, K.; Vik, Unni; Veldre, Vilmar; Henrik Nilsson, R.

doi:10.1111/2041-210x.12073

Public

Improved software detection and extraction of ITS1 and ITS 2 from ribosomal ITS sequences of fungi and other eukaryotes for analysis of environmental sequencing data

Published in Methods in Ecology and Evolution • Jul 19, 2013

Authors:

Johan Bengtsson‐Palme

Martin Ryberg

Martin Hartmann

Abstract

Summary The nuclear ribosomal internal transcribed spacer ( ITS ) region is the primary choice for molecular identification of fungi. Its two highly variable spacers ( ITS 1 and ITS 2) are usually species specific, whereas the intercalary 5.8S gene is highly conserved. For sequence clustering and bl...

Subject

Biology

Computational biology

Sequence analysis

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