Variable‐angle total internal reflection fluorescence microscopy (VA‐TIRFM): realization and application of a compact illumination device

Stock, Karl; Sailer, R.; S. L. Strauß, Wolfgang; Lyttek, Marco; Steiner, R.; Schneckenburger, Herbert

doi:10.1046/j.1365-2818.2003.01200.x

Public

Variable‐angle total internal reflection fluorescence microscopy (VA‐TIRFM): realization and application of a compact illumination device

Published in Journal of Microscopy • Jul 1, 2003

Authors:

Karl Stock

R. Sailer

Wolfgang S. L. Strauß

Abstract

A novel compact illumination device in variable-angle total internal reflection fluorescence microscopy (VA-TIRFM) is described. This device replaces the standard condensor of an upright microscope. Light from different laser sources is delivered via a monomode fibre and focused onto identical parts...

Subject

Total internal reflection fluorescence microscope

Microscopy

Microscope

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