Liquid chromatography/mass spectrometry characterization of Escherichia coli and Shigella species.

A Everley, Robert; M Mott, Tiffany; A Wyatt, Shane; M Toney, Denise; R Croley, Timothy

doi:10.1016/j.jasms.2008.07.003

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Liquid chromatography/mass spectrometry characterization of Escherichia coli and Shigella species.

Shared by NobleBlocks on Nov 1, 2008 • 12:00 AM UTC

Authors:

Robert A Everley

Tiffany M Mott

Shane A Wyatt

Abstract

Liquid chromatography/quadrupole time of flight mass spectrometry (LC/QTOF MS) utilizing electrospray ionization was employed to monitor protein expression in Escherichia coli and Shigella organisms. Comparison with MALDI/TOF-MS revealed more proteins, particularly above 15 kDa. A combination of aut...

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