Liquid chromatography/mass spectrometry characterization of <i>Escherichia coli</i> and <i>Shigella</i> species

A. Everley, Robert; M. Mott, Tiffany; A. Wyatt, Shane; Toney, Denise; R. Croley, Timothy

doi:10.1016/j.jasms.2008.07.003

Public

Liquid chromatography/mass spectrometry characterization of Escherichia coli and Shigella species

Published in Journal of the American Society for Mass Spectrometry • Jul 17, 2008

Authors:

Robert A. Everley

Tiffany M. Mott

Shane A. Wyatt

Abstract

Liquid chromatography/quadrupole time of flight mass spectrometry (LC/QTOF MS) utilizing electrospray ionization was employed to monitor protein expression in Escherichia coli and Shigella organisms. Comparison with MALDI/TOF-MS revealed more proteins, particularly above 15 kDa. A combination of aut...

Subject

Chemistry

Chromatography

Mass spectrometry

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