Fourth-Generation Epac-Based FRET Sensors for cAMP Feature Exceptional Brightness, Photostability and Dynamic Range: Characterization of Dedicated Sensors for FLIM, for Ratiometry and with High Affinity

Klarenbeek, Jeffrey; Goedhart, Joachim; van Batenburg, Aernoud; Groenewald, Daniella; Jalink, Kees

doi:10.1371/journal.pone.0122513

Public

Fourth-Generation Epac-Based FRET Sensors for cAMP Feature Exceptional Brightness, Photostability and Dynamic Range: Characterization of Dedicated Sensors for FLIM, for Ratiometry and with High Affinity

Published in PLoS ONE • Apr 14, 2015

Authors:

Jeffrey Klarenbeek

Joachim Goedhart

Aernoud van Batenburg

Abstract

Epac-based FRET sensors have been widely used for the detection of cAMP concentrations in living cells. Originally developed by us as well as others, we have since then reported several important optimizations that make these sensors favourite among many cell biologists. We here report cloning and c...

Subject

Förster resonance energy transfer

Dynamic range

Fluorescence

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