Quantification of mitogen induced human lymphocyte proliferation: comparison of alamarBlue assay to 3H-thymidine incorporation assay.

Fries R, de; M, Mitsuhashi

doi:10.1002/jcla.1860090203

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Quantification of mitogen induced human lymphocyte proliferation: comparison of alamarBlue assay to 3H-thymidine incorporation assay.

Shared by NobleBlocks on Jan 1, 1995 • 12:00 AM UTC

Authors:

de Fries R

Mitsuhashi M

Abstract

Proliferation of human lymphocytes in response to various stimuli has traditionally been assessed by measuring uptake of radiolabeled nucleotides such as 3H-thymidine. We have evaluated a fluorometric assay, which uses the commercially available reagent, alamarBlue, as a potential substitute for the...

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