Quantification of mitogen induced human lymphocyte proliferation: Comparison of alamarblue<sup>tm</sup> assay to <sup>3</sup>h‐thymidine incorporation assay

De Fries, Ricarda; Mitsuhashi, Masato

doi:10.1002/jcla.1860090203

Public

Quantification of mitogen induced human lymphocyte proliferation: Comparison of alamarbluetm assay to 3h‐thymidine incorporation assay

Published in Journal of Clinical Laboratory Analysis • Jan 1, 1995

Authors:

Ricarda De Fries

Masato Mitsuhashi

Abstract

Proliferation of human lymphocytes in response to various stimuli has traditionally been assessed by measuring uptake of radiolabeled nucleotides such as 3H-thymidine. We have evaluated a fluorometric assay, which uses the commercially available reagent, alamarBlue, as a potential substitute for the...

Subject

Peripheral blood mononuclear cell

Thymidine

Concanavalin A

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