Cryopreservation of 250 strains of Chlorococcales by the method of two-step cooling

Abstract A two-step cooling technique is described which allows the successful recovery (>50% viability) of 252 strains of Chlorococcales following freezing to and thawing from -196°C. The method was unsatisfactory with only 32 strains. Survival of cells at -196°C was demonstrated to be independent ... View More